Restriction map for
pFN21AB8981
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BanI BanII BbeI BbrPI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaJI
BsaWI BseAI BseRI BsiEI BsiWI
BsiYI BslI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HpaII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RsaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Alw44I 32 GTGCAC G^TGCAC
AlwI 75 GGATC GGATC(4/5)
AlwNI 1046 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 32 GTGCAC G^TGCAC
Asp700I 165 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 32 GTGCAC G[AT]GC[AT]^C
BalI 1053 TGGCCA TGG^CCA
BbsI 885 GTCTTC GAAGAC(2/6)
BmyI 32 GTGCAC G[AGT]GC[ACT]^C
BpuAI 885 GTCTTC GAAGAC(2/6)
BsiHKAI 32 GTGCAC G[AT]GC[AT]^C
BsmFI 778 GTCCC GGGAC(10/14)
BsmI 402 GAATGC GAATGC(1/-1)
Bsp1286I 32 GTGCAC G[AGT]GC[ACT]^C
BspHI 78 TCATGA T^CATGA
BsrI 466 CCAGT ACTGG(1/-1)
BstAPI 34 GCACTGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DpnII 76 GATC ^GATC
DraIII 35 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1053 TGGCCA [CT]^GGCC[GA]
EarI 444 CTCTTC CTCTTC(1/4)
EcoRI 471 GAATTC G^AATTC
HindIII 217 AAGCTT A^AGCTT
HinfI 1092 GAATC G^A[ACGT]TC
HpaI 433 GTTAAC GTT^AAC
Ksp632I 444 CTCTTC CTCTTC(1/4)
MaeII 87 ACGT A^CGT
MboI 76 GATC ^GATC
MluNI 1053 TGGCCA TGG^CCA
MscI 1053 TGGCCA TGG^CCA
MseI 434 TTAA T^TAA
NdeII 76 GATC ^GATC
NheI 153 GCTAGC G^CTAGC
RcaI 78 TCATGA T^CATGA
SapI 443 GCTCTTC GCTCTTC(1/4)
Sau3AI 76 GATC ^GATC
StuI 211 AGGCCT AGG^CCT
TfiI 1092 GAATC G^A[AT]TC
Tru9I 434 TTAA T^TAA
XmnI 165 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 13 CCGC CCGC(-3/-1)
818 CCGC
BfaI 154 CTAG C^TAG
805 CTAG
BsgI 196 CTGCAC GTGCAG(16/14)
980 CTGCAC
BssKI 145 CCTGG ^CC[ACGT]GG
1051 CCTGG
BstF5I 277 CATCC GGATG(2/0)
815 CATCC
BstNI 145 CCTGG CC^[AT]GG
1051 CCTGG
Cac8I 43 GCTTGC GC[ACGT]^[ACGT]GC
153 GCTAGC
EcoRII 145 CCTGG ^CC[AT]GG
1051 CCTGG
FokI 277 CATCC GGATG(9/13)
815 CATCC
HaeIII 212 GGCC GG^CC
1054 GGCC
HincII 301 GTCAAC GT[CT]^[GA]AC
433 GTTAAC
HindII 301 GTCAAC GT[CT]^[GA]AC
433 GTTAAC
MaeI 154 CTAG C^TAG
805 CTAG
MvaI 145 CCTGG CC^[AT]GG
1051 CCTGG
MwoI 34 GCACTGAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
454 GCTACATTAGC
NlaIII 79 CATG CATG^
1119 CATG
ScrFI 145 CCTGG CC^[ACGT]GG
1051 CCTGG
SfaNI 26 GCATC GCATC(5/9)
99 GCATC
SfcI 207 CTGTAG C^T[GA][CT]AG
449 CTGTAG
TaqI 66 TCGA T^CGA
1071 TCGA
TthHB8I 66 TCGA T^CGA
1071 TCGA
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AcsI AluI ApoI BbvCI BbvI
BsaI BsmAI DdeI Fnu4HI HphI
ItaI MaeIII MboII MnlI MspA1I
PvuII TseI Tsp45I Tsp509I TspRI