(Circular) (Six-base) MAPSORT of: pFN21AB8981.seq Check: 7456 from: 1 to: 5836 pFN21AB8981 5836 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2964 3033 3899 5283 5551 1632 Size: 1332 69 866 1384 268 1917 Fragments arranged by size: 1917 1384 1332 866 268 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 3056 3579 5151 3056 Size: 523 1572 3741 Fragments arranged by size: 3741 1572 523 ApaLI G'TGCA_C Cuts at: 2037 3893 5049 2037 Size: 1856 1156 2824 Fragments arranged by size: 2824 1856 1156 ApoI r'AATT_y Cuts at: 1493 2252 2334 2476 3069 3145 3371 3523 5632 Size: 759 82 142 593 76 226 152 2109 Cuts at: 5632 1493 Size: 1697 Fragments arranged by size: 2109 1697 759 593 226 152 142 82 76 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 3161 1870 Size: 73 1218 4545 Fragments arranged by size: 4545 1218 73 BamHI G'GATC_C Cuts at: 3166 3533 3166 Size: 367 5469 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3157 4612 618 Size: 325 217 627 1370 1455 1842 Fragments arranged by size: 1842 1455 1370 627 325 217 BanII G_rGCy'C Cuts at: 1391 3155 1391 Size: 1764 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2883 928 Size: 971 984 3881 Fragments arranged by size: 3881 984 971 BbvCI CC'TCA_GC Cuts at: 2551 2620 2689 2758 2827 2551 Size: 69 69 69 69 5560 Fragments arranged by size: 5560 69 69 69 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 1548 Size: 5836 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4447 136 Size: 107 122 71 631 605 2775 1525 Fragments arranged by size: 2775 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 3253 3253 Size: 5836 Bme1580I G_kGCm'C Cuts at: 1790 2041 3897 5053 1790 Size: 251 1856 1156 2573 Fragments arranged by size: 2573 1856 1156 251 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3567 4525 442 Size: 416 53 83 399 24 2150 958 1753 Fragments arranged by size: 2150 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2162 1056 Size: 1106 4730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4496 1239 Size: 3257 2579 Bpu10I CC'TnA_GC Cuts at: 2220 2551 2620 2689 2758 2827 2220 Size: 331 69 69 69 69 5229 Fragments arranged by size: 5229 331 69 69 69 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 2627 2696 2765 2834 2903 4499 Size: 617 1059 69 69 69 69 69 1596 Cuts at: 4499 882 Size: 2219 Fragments arranged by size: 2219 1596 1059 617 69 69 69 69 69 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3643 4025 4660 275 Size: 53 83 186 936 2110 382 635 1451 Fragments arranged by size: 2110 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2521 2551 2590 2620 2659 Size: 30 165 30 1118 30 39 30 39 Cuts at: 2659 2689 2728 2758 2866 2896 3037 3067 1178 Size: 30 39 30 108 30 141 30 3947 Fragments arranged by size: 3947 1118 165 141 108 39 39 39 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5836 BseYI C'CCAG_C Cuts at: 2482 2896 5039 5535 2482 Size: 414 2143 496 2783 Fragments arranged by size: 2783 2143 496 414 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2970 1163 Size: 573 450 784 4029 Fragments arranged by size: 4029 784 573 450 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2041 3897 3982 5053 2041 Size: 1856 85 1071 2824 Fragments arranged by size: 2824 1856 1071 85 BsmI GAATG_Cn' Cuts at: 2413 3347 3440 2413 Size: 934 93 4809 Fragments arranged by size: 4809 934 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2041 3155 3897 3982 5053 1391 Size: 399 251 1114 742 85 1071 2174 Fragments arranged by size: 2174 1114 1071 742 399 251 85 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 2083 3725 5455 2083 Size: 1642 1730 2464 Fragments arranged by size: 2464 1730 1642 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3191 844 Size: 2347 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4480 4667 1700 Size: 110 2670 187 2869 Fragments arranged by size: 2869 2670 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 3896 4908 3896 Size: 1012 4824 BstAPI GCAn_nnn'nTGC Cuts at: 794 2045 794 Size: 1251 4585 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstYI r'GATC_y Cuts at: 1972 3166 3533 3918 3935 5387 5473 5485 5831 Size: 1194 367 385 17 1452 86 12 346 Cuts at: 5831 1972 Size: 1977 Fragments arranged by size: 1977 1452 1194 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5284 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 2305 3352 4146 4166 2305 Size: 1047 794 20 3975 Fragments arranged by size: 3975 1047 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DraIII CAC_nnn'GTG Cuts at: 2045 2045 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3058 3283 4172 Size: 54 1425 50 275 18 1228 225 889 Cuts at: 4172 5801 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1228 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2455 3766 1353 Size: 1102 1311 3423 Fragments arranged by size: 3423 1311 1102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2812 4423 4797 4943 387 Size: 1064 138 1223 1611 374 146 1280 Fragments arranged by size: 1611 1280 1223 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2964 3033 3899 4496 5283 5551 1239 Size: 393 1332 69 866 597 787 268 1524 Fragments arranged by size: 1524 1332 866 787 597 393 268 69 EcoRI G'AATT_C Cuts at: 2476 3145 3523 2476 Size: 669 378 4789 Fragments arranged by size: 4789 669 378 FspI TGC'GCA Cuts at: 4342 4342 Size: 5836 HaeII r_GCGC'y Cuts at: 1678 4983 1678 Size: 3305 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2804 2836 4477 4509 4551 Size: 32 135 32 1232 32 1641 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 1641 1232 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2308 2440 3180 3426 669 Size: 988 281 370 132 740 246 3079 Fragments arranged by size: 3079 988 740 370 281 246 132 HindIII A'AGCT_T Cuts at: 748 2222 748 Size: 1474 4362 HpaI GTT'AAC Cuts at: 2440 3426 2440 Size: 986 4850 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3060 10 Size: 54 1425 343 1228 2786 Fragments arranged by size: 2786 1425 1228 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3794 4153 4312 518 Size: 648 2628 359 159 2042 Fragments arranged by size: 2628 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2020 2504 2573 2642 2711 2780 2849 2918 Size: 414 484 69 69 69 69 69 69 Cuts at: 2918 2984 3252 3929 5077 5322 1606 Size: 66 268 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 484 414 268 245 69 69 69 69 69 69 66 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5284 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 2158 1052 Size: 1106 4730 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 3194 4739 1132 Size: 97 1965 1545 2229 Fragments arranged by size: 2229 1965 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5836 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5836 PfoI T'CCnGG_A Cuts at: 2149 2149 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PstI C_TGCA'G Cuts at: 830 3188 830 Size: 2358 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2504 2573 2642 2711 2780 2849 2918 2984 2504 Size: 69 69 69 69 69 69 66 5356 Fragments arranged by size: 5356 69 69 69 69 69 69 66 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SapI GCTCTTCn'nnn_ Cuts at: 2455 2455 Size: 5836 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 4084 5796 4084 Size: 1712 4124 SfcI C'TryA_G Cuts at: 826 1045 2212 2454 3184 4319 5000 5191 826 Size: 219 1167 242 730 1135 681 191 1471 Fragments arranged by size: 1471 1167 1135 730 681 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 2218 1517 Size: 701 5135 StyI C'CwwG_G Cuts at: 513 1065 3275 513 Size: 552 2210 3074 Fragments arranged by size: 3074 2210 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2134 2527 2596 2665 2734 2803 2872 Size: 1255 554 393 69 69 69 69 69 Cuts at: 2872 3001 3004 3863 3880 4033 4218 4663 325 Size: 129 3 859 17 153 185 445 1498 Fragments arranged by size: 1498 1255 859 554 445 393 185 153 129 69 69 69 69 69 17 3 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4082 5794 96 Size: 274 80 33 51 1443 2105 1712 138 Fragments arranged by size: 2105 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5796 5828 5796 Size: 32 5804 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5836 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 2174 3965 2174 Size: 1791 4045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI