(Linear) (Six-base) MAPSORT of: KIBB8981.seq Check: 6277 from: 1 to: 1131 KIBB8981.orf With 182 enzymes: SgfI * July 15, 2010 18:32 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 959 1028 1131 Size: 959 69 103 Fragments arranged by size: 959 103 69 AlwNI CAG_nnn'CTG Cuts at: 0 1051 1131 Size: 1051 80 ApaLI G'TGCA_C Cuts at: 0 32 1131 Size: 32 1099 ApoI r'AATT_y Cuts at: 0 247 329 471 1064 1131 Size: 247 82 142 593 67 Fragments arranged by size: 593 247 142 82 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 878 1131 Size: 878 253 BbvCI CC'TCA_GC Cuts at: 0 546 615 684 753 822 1131 Size: 546 69 69 69 69 309 Fragments arranged by size: 546 309 69 69 69 69 Bme1580I G_kGCm'C Cuts at: 0 36 1131 Size: 36 1095 BmtI G_CTAG'C Cuts at: 0 157 1131 Size: 157 974 Bpu10I CC'TnA_GC Cuts at: 0 215 546 615 684 753 822 1131 Size: 215 331 69 69 69 69 309 Fragments arranged by size: 331 309 215 69 69 69 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 553 622 691 760 829 898 1131 Size: 553 69 69 69 69 69 233 Fragments arranged by size: 553 233 69 69 69 69 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 516 546 585 615 654 684 723 753 Size: 516 30 39 30 39 30 39 30 Cuts at: 753 861 891 1032 1062 1131 Size: 108 30 141 30 69 Fragments arranged by size: 516 141 108 69 39 39 39 30 30 30 30 30 30 BseYI C'CCAG_C Cuts at: 0 477 891 1131 Size: 477 414 240 Fragments arranged by size: 477 414 240 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 965 1131 Size: 181 784 166 Fragments arranged by size: 784 181 166 BsiHKAI G_wGCw'C Cuts at: 0 36 1131 Size: 36 1095 BsmI GAATG_Cn' Cuts at: 0 408 1131 Size: 408 723 Bsp1286I G_dGCh'C Cuts at: 0 36 1131 Size: 36 1095 BspHI T'CATG_A Cuts at: 0 78 1131 Size: 78 1053 BstAPI GCAn_nnn'nTGC Cuts at: 0 40 1131 Size: 40 1091 BtsI GCAGTG_nn' Cuts at: 0 300 1131 Size: 300 831 DraIII CAC_nnn'GTG Cuts at: 0 40 1131 Size: 40 1091 EaeI y'GGCC_r Cuts at: 0 1053 1131 Size: 1053 78 EarI CTCTTCn'nnn_ Cuts at: 0 450 1131 Size: 450 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 807 1131 Size: 807 324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 959 1028 1131 Size: 959 69 103 Fragments arranged by size: 959 103 69 EcoRI G'AATT_C Cuts at: 0 471 1131 Size: 471 660 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 799 831 1131 Size: 799 32 300 Fragments arranged by size: 799 300 32 HincII GTy'rAC Cuts at: 0 303 435 1131 Size: 303 132 696 Fragments arranged by size: 696 303 132 HindIII A'AGCT_T Cuts at: 0 217 1131 Size: 217 914 HpaI GTT'AAC Cuts at: 0 435 1131 Size: 435 696 MscI TGG'CCA Cuts at: 0 1055 1131 Size: 1055 76 MspA1I CmG'CkG Cuts at: 0 15 499 568 637 706 775 844 913 Size: 15 484 69 69 69 69 69 69 Cuts at: 913 979 1131 Size: 66 152 Fragments arranged by size: 484 152 69 69 69 69 69 69 66 15 NheI G'CTAG_C Cuts at: 0 153 1131 Size: 153 978 PfoI T'CCnGG_A Cuts at: 0 144 1131 Size: 144 987 PvuII CAG'CTG Cuts at: 0 499 568 637 706 775 844 913 979 Size: 499 69 69 69 69 69 69 66 Cuts at: 979 1131 Size: 152 Fragments arranged by size: 499 152 69 69 69 69 69 69 66 SapI GCTCTTCn'nnn_ Cuts at: 0 450 1131 Size: 450 681 SfcI C'TryA_G Cuts at: 0 207 449 1131 Size: 207 242 682 Fragments arranged by size: 682 242 207 StuI AGG'CCT Cuts at: 0 213 1131 Size: 213 918 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 129 522 591 660 729 798 867 996 Size: 129 393 69 69 69 69 69 129 Cuts at: 996 999 1131 Size: 3 132 Fragments arranged by size: 393 132 129 129 69 69 69 69 69 3 XmnI GAAnn'nnTTC Cuts at: 0 169 1131 Size: 169 962 Enzymes that do cut: AcuI AlwNI ApaLI ApoI BbsI BbvCI Bme1580I BmtI Bpu10I BsaI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BstAPI BtsI DraIII EaeI EarI EciI Eco57MI EcoRI Hin4I HincII HindIII HpaI MscI MspA1I NheI PfoI PvuII SapI SfcI StuI TaqII XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI ZraI