Restriction map for
pFN21AB6668
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccII AccIII AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BciVI BclI BcnI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EarI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT14I FauI FbaI FseI FspI
HaeII HapII HhaI HinP1I HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SacI SacII SapI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI StyI SwaI Van91I XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AclI 176 AACGTT AA^CGTT
AfaI 992 GTAC GT^AC
AflIII 54 ACATGT A^C[GA][CT]GT
AhdI 728 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1101 GATCC GGATC(4/5)
AspEI 728 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 477 GGCACC G^G[CT][GA]CC
BcgI 15 GCAGGGTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 882 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1063 CTCCAG CTGGAG(16/14)
BsaI 1035 GGTCTC GGTCTC(1/5)
BsgI 1308 GTGCAG GTGCAG(16/14)
BspLU11I 54 ACATGT A^CATGT
BspMI 11 ACCTGC ACCTGC(4/8)
BstEII 453 GGTGACC G^GT[ACGT]ACC
BstPI 453 GGTGACC G^GT[ACGT]ACC
BstXI 524 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 289 GCTGGC GC[ACGT]^[ACGT]GC
DraI 165 TTTAAA TTT^AAA
Eam1105I 728 GACAAGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 453 GGTGACC G^GT[ACGT]ACC
EcoT22I 275 ATGCAT ATGCA^T
MaeII 177 ACGT A^CGT
MslI 829 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1216 CCGCTG C[AC]G^C[GT]G
NsiI 275 ATGCAT ATGCA^T
NspI 54 ACATGT [GA]CATG^[CT]
Psp1406I 176 AACGTT AA^CGTT
RsaI 992 GTAC GT^AC
SalI 313 GTCGAC G^TCGAC
SfaNI 696 GATGC GCATC(5/9)
SmlI 987 CTCAAG C^T[CT][GA]AG
StuI 773 AGGCCT AGG^CCT
XbaI 1260 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 313 GTCGAC GT^[AC][GT]AC
1332 GTCTAC
AciI 1216 CCGC CCGC(-3/-1)
888 GCGG
AcyI 733 GGCGTC G[GA]^CG[CT]C
1227 GACGCC
BalI 291 TGGCCA TGG^CCA
743 TGGCCA
BbvCI 142 GCTGAGG CCTCAGC(-5/-2)
1288 GCTGAGG
BmyI 476 GGGCAC G[AGT]GC[ACT]^C
1254 GTGCCC
BsaHI 733 GGCGTC G[GA]^CG[CT]C
1227 GACGCC
BseRI 352 GAGGAG GAGGAG(10/8)
1204 GAGGAG
BsmAI 1120 GAGAC GTCTC(1/5)
1036 GTCTC
BsmFI 42 GTCCC GGGAC(10/14)
908 GTCCC
Bsp1286I 476 GGGCAC G[AGT]GC[ACT]^C
1254 GTGCCC
BsrI 160 CCAGT ACTGG(1/-1)
883 CCAGT
EaeI 291 TGGCCA [CT]^GGCC[GA]
743 TGGCCA
HgaI 1227 GACGC GACGC(5/10)
734 GCGTC
Hin1I 733 GGCGTC G[GA]^CG[CT]C
1227 GACGCC
HincII 313 GTCGAC GT[CT]^[GA]AC
541 GTCAAC
HindII 313 GTCGAC GT[CT]^[GA]AC
541 GTCAAC
MluNI 291 TGGCCA TGG^CCA
743 TGGCCA
MscI 291 TGGCCA TGG^CCA
743 TGGCCA
MseI 166 TTAA T^TAA
554 TTAA
PpuMI 436 GGGTCCT [GA]G^G[AT]CC[CT]
1208 AGGACCC
SbfI 12 CCTGCAGG CCTGCA^GG
468 CCTGCAGG
Sse8387I 12 CCTGCAGG CCTGCA^GG
468 CCTGCAGG
TfiI 464 GAATC G^A[AT]TC
646 GATTC
Tru9I 166 TTAA T^TAA
554 TTAA
Tsp509I 558 AATT ^AATT
1157 AATT
XcmI 1136 CCAGGGAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1232 CCATGAACAGCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AluI AlwNI AspI AvaII BbsI
BbvI BfaI BglII BpuAI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
PflFI PleI PstI Sau3AI Sau96I
ScrFI SfcI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I XhoII