(Circular) (Six-base) MAPSORT of: pFN21AB6668.seq Check: 6942 from: 1 to: 6064 pFN21AB6668 6064 bp With 182 enzymes: SgfI * October 20, 2009 10:58 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3874 278 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2319 3338 3407 1937 Size: 382 1019 69 4594 Fragments arranged by size: 4594 1019 382 69 Acc65I G'GTAC_C Cuts at: 3385 3385 Size: 6064 AclI AA'CG_TT Cuts at: 1563 2182 4191 4564 1563 Size: 619 2009 373 3063 Fragments arranged by size: 3063 2009 619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 2996 3096 4127 5511 5779 1632 Size: 575 789 100 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 789 575 268 100 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5867 AflIII A'CryG_T Cuts at: 1203 2059 4963 5940 1203 Size: 856 2904 977 1327 Fragments arranged by size: 2904 1327 977 856 AgeI A'CCGG_T Cuts at: 4895 4895 Size: 6064 AhdI GACnn_n'nnGTC Cuts at: 2738 4793 2738 Size: 2055 4009 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6064 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6064 AlwNI CAG_nnn'CTG Cuts at: 2105 2704 3277 3807 5379 2105 Size: 599 573 530 1572 2790 Fragments arranged by size: 2790 1572 599 573 530 ApaLI G'TGCA_C Cuts at: 4121 5277 4121 Size: 1156 4908 ApoI r'AATT_y Cuts at: 1493 3373 3599 3751 5860 1493 Size: 1880 226 152 2109 1697 Fragments arranged by size: 2109 1880 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4618 160 Size: 872 3586 1606 Fragments arranged by size: 3586 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 AvaI C'yCGr_G Cuts at: 1870 1943 3389 1870 Size: 73 1446 4545 Fragments arranged by size: 4545 1446 73 BamHI G'GATC_C Cuts at: 3394 3761 3394 Size: 367 5697 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2482 3385 4840 618 Size: 325 217 627 695 903 1455 1842 Fragments arranged by size: 1842 1455 903 695 627 325 217 BanII G_rGCy'C Cuts at: 1391 3383 1391 Size: 1992 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2206 2726 3295 928 Size: 971 307 520 569 3697 Fragments arranged by size: 3697 971 569 520 307 BbvCI CC'TCA_GC Cuts at: 2148 3294 2148 Size: 1146 4918 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2009 2043 4255 4289 1903 Size: 34 72 34 2212 34 3678 Fragments arranged by size: 3678 2212 72 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3956 5166 3956 Size: 1210 4854 BclI T'GATC_A Cuts at: 1548 1548 Size: 6064 BfrBI ATG'CAT Cuts at: 2282 2282 Size: 6064 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2893 4675 136 Size: 107 122 71 631 605 1221 1782 1525 Fragments arranged by size: 1782 1525 1221 631 605 122 107 71 BglII A'GATC_T Cuts at: 2490 2809 3321 6059 2490 Size: 319 512 2738 2495 Fragments arranged by size: 2738 2495 512 319 BlpI GC'TnA_GC Cuts at: 3481 3481 Size: 6064 Bme1580I G_kGCm'C Cuts at: 1790 2485 3263 4125 5281 1790 Size: 695 778 862 1156 2573 Fragments arranged by size: 2573 1156 862 778 695 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6064 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3795 4753 442 Size: 416 53 83 399 24 2378 958 1753 Fragments arranged by size: 2378 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6064 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3053 4724 1239 Size: 1814 1671 2579 Fragments arranged by size: 2579 1814 1671 Bpu10I CC'TnA_GC Cuts at: 2148 3294 2148 Size: 1146 4918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2977 4189 5054 5352 2977 Size: 1212 865 298 3689 Fragments arranged by size: 3689 1212 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3046 4727 882 Size: 617 1547 1681 2219 Fragments arranged by size: 2219 1681 1547 617 BsaAI yAC'GTr Cuts at: 493 1204 3855 493 Size: 711 2651 2702 Fragments arranged by size: 2702 2651 711 BsaBI GATnn'nnATC Cuts at: 6058 6058 Size: 6064 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2739 3233 3871 4253 Size: 53 83 186 936 1206 494 638 382 Cuts at: 4253 4888 275 Size: 635 1451 Fragments arranged by size: 1451 1206 936 638 635 494 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4497 4895 5169 5316 5727 4497 Size: 398 274 147 411 4834 Fragments arranged by size: 4834 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5839 Fragments arranged by size: 5839 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 3224 1186 Size: 1186 852 4026 Fragments arranged by size: 4026 1186 852 BseYI C'CCAG_C Cuts at: 2068 5267 5763 2068 Size: 3199 496 2369 Fragments arranged by size: 3199 2369 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3334 1163 Size: 573 1598 3893 Fragments arranged by size: 3893 1598 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3514 4275 5303 1540 Size: 275 186 1513 761 1028 2301 Fragments arranged by size: 2301 1513 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4125 4210 5281 4125 Size: 85 1071 4908 Fragments arranged by size: 4908 1071 85 BsmI GAATG_Cn' Cuts at: 3575 3668 3575 Size: 93 5971 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2485 3263 3383 4125 4210 5281 1391 Size: 399 695 778 120 742 85 1071 2174 Fragments arranged by size: 2174 1071 778 742 695 399 120 85 BspEI T'CCGG_A Cuts at: 5727 5727 Size: 6064 BspHI T'CATG_A Cuts at: 3953 5683 3953 Size: 1730 4334 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 3419 844 Size: 1181 1394 3489 Fragments arranged by size: 3489 1394 1181 BsrBI CCG'CTC Cuts at: 1394 1823 3951 1394 Size: 429 2128 3507 Fragments arranged by size: 3507 2128 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4559 4733 65 Size: 1183 3311 174 1396 Fragments arranged by size: 3311 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4708 4895 1700 Size: 110 2898 187 2869 Fragments arranged by size: 2898 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6064 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6064 BssSI C'ACGA_G Cuts at: 4124 5136 4124 Size: 1012 5052 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6064 BstBI TT'CG_AA Cuts at: 4868 4868 Size: 6064 BstEII G'GTnAC_C Cuts at: 1184 2458 1184 Size: 1274 4790 BstXI CCAn_nnnn'nTGG Cuts at: 2536 2536 Size: 6064 BstYI r'GATC_y Cuts at: 1972 2490 2809 3105 3321 3394 3761 4146 4163 Size: 518 319 296 216 73 367 385 17 Cuts at: 4163 5615 5701 5713 6059 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 518 385 367 346 319 296 216 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4573 Fragments arranged by size: 4573 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5325 Fragments arranged by size: 5325 683 56 BtsI GCAGTG_nn' Cuts at: 2404 3580 4374 4394 2404 Size: 1176 794 20 4074 Fragments arranged by size: 4074 1176 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6064 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6029 DraI TTT'AAA Cuts at: 2172 3368 2172 Size: 1196 4868 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5071 809 Size: 507 3755 1802 Fragments arranged by size: 3755 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2296 2748 3511 Size: 54 1425 50 275 18 466 452 763 Cuts at: 3511 4400 6029 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 763 466 452 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3511 1537 Size: 275 1699 4090 Fragments arranged by size: 4090 1699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3994 1353 Size: 2641 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4651 5025 5171 387 Size: 1064 138 3062 374 146 1280 Fragments arranged by size: 3062 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2996 3053 3096 4127 4724 5511 Size: 393 575 789 57 43 1031 597 787 Cuts at: 5511 5779 1239 Size: 268 1524 Fragments arranged by size: 1524 1031 789 787 597 575 393 268 57 43 EcoNI CCTnn'n_nnAGG Cuts at: 2199 2357 2660 3068 2199 Size: 158 303 408 5195 Fragments arranged by size: 5195 408 303 158 EcoO109I rG'GnC_Cy Cuts at: 2442 2611 3214 2442 Size: 169 603 5292 Fragments arranged by size: 5292 603 169 EcoRI G'AATT_C Cuts at: 3373 3751 3373 Size: 378 5686 FspI TGC'GCA Cuts at: 4570 4570 Size: 6064 HaeII r_GCGC'y Cuts at: 1678 5211 1678 Size: 3533 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2078 2110 2724 2756 4705 Size: 32 135 32 506 32 614 32 1949 Cuts at: 4705 4737 4779 4811 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1949 614 506 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 2548 3408 3654 669 Size: 988 281 382 228 860 246 3079 Fragments arranged by size: 3079 988 860 382 281 246 228 HindIII A'AGCT_T Cuts at: 748 748 Size: 6064 HpaI GTT'AAC Cuts at: 3654 3654 Size: 6064 KpnI G_GTAC'C Cuts at: 3389 3389 Size: 6064 MfeI C'AATT_G Cuts at: 3663 3663 Size: 6064 MluI A'CGCG_T Cuts at: 5940 5940 Size: 6064 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5178 5362 1305 Size: 3873 184 2007 Fragments arranged by size: 3873 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2298 2750 10 Size: 54 1425 343 466 452 3324 Fragments arranged by size: 3324 1425 466 452 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2838 4022 4381 4540 518 Size: 648 1672 1184 359 159 2042 Fragments arranged by size: 2042 1672 1184 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3223 3480 4157 5305 5550 1606 Size: 1617 257 677 1148 245 2120 Fragments arranged by size: 2120 1617 1148 677 257 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4573 Fragments arranged by size: 4573 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6064 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6064 NotI GC'GGCC_GC Cuts at: 3511 3511 Size: 6064 NsiI A_TGCA'T Cuts at: 2284 2284 Size: 6064 NspI r_CATG'y Cuts at: 1132 1229 2063 3422 4967 1132 Size: 97 834 1359 1545 2229 Fragments arranged by size: 2229 1545 1359 834 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6064 PciI A'CATG_T Cuts at: 2059 4963 2059 Size: 2904 3160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6064 PfoI T'CCnGG_A Cuts at: 2535 2949 3069 2535 Size: 414 120 5530 Fragments arranged by size: 5530 414 120 PmeI GTTT'AAAC Cuts at: 3368 3368 Size: 6064 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4133 4165 5646 5678 4133 Size: 32 1481 32 4519 Fragments arranged by size: 4519 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2442 3214 2442 Size: 772 5292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6064 PsiI TTA'TAA Cuts at: 3634 3634 Size: 6064 PstI C_TGCA'G Cuts at: 830 2022 2043 2478 2512 2790 3416 830 Size: 1192 21 435 34 278 626 3478 Fragments arranged by size: 3478 1192 626 435 278 34 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6064 SalI G'TCGA_C Cuts at: 1936 2318 3406 1936 Size: 382 1088 4594 Fragments arranged by size: 4594 1088 382 SbfI CC_TGCA'GG Cuts at: 2022 2478 3416 2022 Size: 456 938 4670 Fragments arranged by size: 4670 938 456 ScaI AGT'ACT Cuts at: 4312 6024 4312 Size: 1712 4352 SfcI C'TryA_G Cuts at: 826 1045 2018 2039 2474 2508 2728 2786 3412 Size: 219 973 21 435 34 220 58 626 Cuts at: 3412 4547 5228 5419 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 973 681 626 435 220 219 191 58 34 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6064 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6064 SmaI CCC'GGG Cuts at: 1872 3391 1872 Size: 1519 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2992 4168 5069 5331 820 Size: 197 926 1049 1176 901 262 1553 Fragments arranged by size: 1553 1176 1049 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3855 493 Size: 3362 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6064 SphI G_CATG'C Cuts at: 1132 3422 1132 Size: 2290 3774 SspI AAT'ATT Cuts at: 5 52 3988 5 Size: 47 3936 2081 Fragments arranged by size: 3936 2081 47 StuI AGG'CCT Cuts at: 1517 2780 1517 Size: 1263 4801 StyI C'CwwG_G Cuts at: 513 1065 2004 3503 513 Size: 552 939 1499 3074 Fragments arranged by size: 3074 1499 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2477 3050 4091 4108 4261 4446 4891 Size: 1255 897 573 1041 17 153 185 445 Cuts at: 4891 325 Size: 1498 Fragments arranged by size: 1498 1255 1041 897 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4310 6022 96 Size: 274 80 33 51 1443 2333 1712 138 Fragments arranged by size: 2333 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6024 6056 6024 Size: 32 6032 Tth111I GACn'n_nGTC Cuts at: 1316 2210 2543 3198 1316 Size: 894 333 655 4182 Fragments arranged by size: 4182 894 655 333 XbaI T'CTAG_A Cuts at: 3265 3400 3265 Size: 135 5929 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3148 3244 3148 Size: 96 5968 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6064 XmaI C'CCGG_G Cuts at: 1870 3389 1870 Size: 1519 4545 XmnI GAAnn'nnTTC Cuts at: 4193 4193 Size: 6064 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3872 276 Size: 53 83 186 936 2338 2468 Fragments arranged by size: 2468 2338 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI