################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbrPI BbvCI BciVI BfaI BfrI BlnI BsaBI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrDI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraIII Eco47III Eco81I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MluI MroI NdeI NheI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SbfI ScaI SexAI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AflIII 1814 ACACGT A^C[GA][CT]GT AgeI 1651 ACCGGT A^CCGGT AhdI 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbsI 270 GAAGAC GAAGAC(2/6) BcgI 2053 GCAGTGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 675 TGATCA T^GATCA BglII 1181 AGATCT A^GATCT BlpI 1255 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1255 GCTGAGC GC^T[ACGT]AGC BpuAI 270 GAAGAC GAAGAC(2/6) BsaI 217 GAGACC GGTCTC(1/5) Bsp1407I 551 TGTACA T^GTACA BsrGI 551 TGTACA T^GTACA BstEII 103 GGTCACC G^GT[ACGT]ACC BstPI 103 GGTCACC G^GT[ACGT]ACC BstXI 1673 CCACGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1255 GCTGAGC GC^T[ACGT]AGC DraI 406 TTTAAA TTT^AAA DrdI 2011 GACGGCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 1285 GACAAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1476 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 103 GGTCACC G^GT[ACGT]ACC EcoRI 829 GAATTC G^AATTC FbaI 675 TGATCA T^GATCA MfeI 199 CAATTG C^AATTG MunI 199 CAATTG C^AATTG NaeI 1389 GCCGGC GCC^GGC NgoMIV 1389 GCCGGC G^CCGGC NruI 1374 TCGCGA TCG^CGA PflMI 2172 CCAGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1651 ACCGGT A^CCGGT SapI 1551 GCTCTTC GCTCTTC(1/4) SfiI 1516 GGCCTGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 1650 CACCGGTG C[GA]^CCGG[CT]G SspBI 551 TGTACA T^GTACA TaqI 1087 TCGA T^CGA TfiI 1669 GATTC G^A[AT]TC TthHB8I 1087 TCGA T^CGA Van91I 2172 CCAGCGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcsI 490 AAATTT [GA]^AATT[CT] 829 GAATTC Alw44I 313 GTGCAC G^TGCAC 1867 GTGCAC AlwNI 1702 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG 1768 CAGGGCCTG ApaLI 313 GTGCAC G^TGCAC 1867 GTGCAC ApoI 490 AAATTT [GA]^AATT[CT] 829 GAATTC AspHI 313 GTGCAC G[AT]GC[AT]^C 1867 GTGCAC BsaAI 684 TACGTG [CT]AC^GT[GA] 1815 CACGTA BsaWI 21 TCCGGT [AT]^CCGG[AT] 1651 ACCGGT BsgI 1558 CTGCAC GTGCAG(16/14) 1450 GTGCAG BsiHKAI 313 GTGCAC G[AT]GC[AT]^C 1867 GTGCAC BsmAI 217 GAGAC GTCTC(1/5) 1657 GTCTC BstYI 18 AGATCC [GA]^GATC[CT] 1181 AGATCT MflI 18 AGATCC [GA]^GATC[CT] 1181 AGATCT MseI 407 TTAA T^TAA 874 TTAA PstI 955 CTGCAG CTGCA^G 2161 CTGCAG Tru9I 407 TTAA T^TAA 874 TTAA XcmI 182 CCAAGCCGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 308 CCAAGGTGCACCTGG XhoII 18 AGATCC [GA]^GATC[CT] 1181 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI ApaI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NotI PleI PpuMI RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StuI StyI TseI Tsp45I Tsp509I TspRI XmaCI XmaI