(Circular) (Six-base) MAPSORT of: pFN21AB6231.seq Check: 4642 from: 1 to: 6934 pFN21AB6231 6934 bp With 182 enzymes: SgfI * March 28, 2012 12:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4744 278 Size: 53 83 186 936 3208 2468 Fragments arranged by size: 3208 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4277 1937 Size: 2340 4594 Acc65I G'GTAC_C Cuts at: 4255 4255 Size: 6934 AclI AA'CG_TT Cuts at: 1563 5061 5434 1563 Size: 3498 373 3063 Fragments arranged by size: 3498 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4997 6381 6649 1632 Size: 3365 1384 268 1917 Fragments arranged by size: 3365 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6737 AflIII A'CryG_T Cuts at: 1203 3819 5833 6810 1203 Size: 2616 2014 977 1327 Fragments arranged by size: 2616 2014 1327 977 AgeI A'CCGG_T Cuts at: 3656 5765 3656 Size: 2109 4825 AhdI GACnn_n'nnGTC Cuts at: 3295 5663 3295 Size: 2368 4566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6934 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3983 1184 Size: 2799 4135 AlwNI CAG_nnn'CTG Cuts at: 3712 3778 4677 6249 3712 Size: 66 899 1572 4397 Fragments arranged by size: 4397 1572 899 66 ApaI G_GGCC'C Cuts at: 2253 3494 4011 2253 Size: 1241 517 5176 Fragments arranged by size: 5176 1241 517 ApaLI G'TGCA_C Cuts at: 2318 3872 4991 6147 2318 Size: 1554 1119 1156 3105 Fragments arranged by size: 3105 1554 1156 1119 ApoI r'AATT_y Cuts at: 1493 2495 2834 4243 4469 4621 6730 1493 Size: 1002 339 1409 226 152 2109 1697 Fragments arranged by size: 2109 1697 1409 1002 339 226 152 AseI AT'TA_AT Cuts at: 160 1032 5488 160 Size: 872 4456 1606 Fragments arranged by size: 4456 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6934 AvaI C'yCGr_G Cuts at: 1870 1943 3164 3270 3424 3517 3664 4066 4259 Size: 73 1221 106 154 93 147 402 193 Cuts at: 4259 1870 Size: 4545 Fragments arranged by size: 4545 1221 402 193 154 147 106 93 73 BamHI G'GATC_C Cuts at: 4264 4631 4264 Size: 367 6567 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2077 2177 2363 3549 3590 Size: 325 217 627 290 100 186 1186 41 Cuts at: 3590 3943 4255 5710 618 Size: 353 312 1455 1842 Fragments arranged by size: 1842 1455 1186 627 353 325 312 290 217 186 100 41 BanII G_rGCy'C Cuts at: 1391 2253 2289 3213 3374 3494 4011 4073 4253 Size: 862 36 924 161 120 517 62 180 Cuts at: 4253 1391 Size: 4072 Fragments arranged by size: 4072 924 862 517 180 161 120 62 36 BbeI G_GCGC'C Cuts at: 2081 2181 3594 2081 Size: 100 1413 5421 Fragments arranged by size: 5421 1413 100 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 928 Size: 971 383 5580 Fragments arranged by size: 5580 971 383 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4047 4081 5125 5159 1903 Size: 34 2110 34 1044 34 3678 Fragments arranged by size: 3678 2110 1044 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4826 6036 4826 Size: 1210 5724 BclI T'GATC_A Cuts at: 1548 2680 1548 Size: 1132 5802 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3528 3701 3836 Size: 107 122 71 631 605 1856 173 135 Cuts at: 3836 4034 4046 4055 5545 136 Size: 198 12 9 1490 1525 Fragments arranged by size: 1856 1525 1490 631 605 198 173 135 122 107 71 12 9 BglII A'GATC_T Cuts at: 3186 6929 3186 Size: 3743 3191 BlpI GC'TnA_GC Cuts at: 3261 4351 3261 Size: 1090 5844 Bme1580I G_kGCm'C Cuts at: 1790 2253 2322 2366 3389 3494 3552 3876 4011 Size: 463 69 44 1023 105 58 324 135 Cuts at: 4011 4995 6151 1790 Size: 984 1156 2573 Fragments arranged by size: 2573 1156 1023 984 463 324 135 105 69 58 44 BmgBI CAC'GTC Cuts at: 1318 3201 3760 1318 Size: 1883 559 4492 Fragments arranged by size: 4492 1883 559 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2592 2869 4665 Size: 416 53 83 399 24 1175 277 1796 Cuts at: 4665 5623 442 Size: 958 1753 Fragments arranged by size: 1796 1753 1175 958 416 399 277 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6934 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 3007 3051 3583 3734 3847 4090 5594 Size: 1106 662 44 532 151 113 243 1504 Cuts at: 5594 1239 Size: 2579 Fragments arranged by size: 2579 1504 1106 662 532 243 151 113 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5059 5924 6222 5059 Size: 865 298 5771 Fragments arranged by size: 5771 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 5597 882 Size: 617 717 3381 2219 Fragments arranged by size: 3381 2219 717 617 BsaAI yAC'GTr Cuts at: 493 1204 2691 3822 4725 493 Size: 711 1487 1131 903 2702 Fragments arranged by size: 2702 1487 1131 903 711 BsaBI GATnn'nnATC Cuts at: 6928 6928 Size: 6934 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2078 2178 3132 3591 Size: 53 83 186 936 545 100 954 459 Cuts at: 3591 3722 4741 5123 5758 275 Size: 131 1019 382 635 1451 Fragments arranged by size: 1451 1019 954 936 635 545 459 382 186 131 100 83 53 BsaWI w'CCGG_w Cuts at: 2026 3656 5367 5765 6039 6186 6597 2026 Size: 1630 1711 398 274 147 411 2363 Fragments arranged by size: 2363 1711 1630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2128 2158 4073 4103 1178 Size: 30 165 30 725 30 1915 30 4009 Fragments arranged by size: 4009 1915 725 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 3167 3221 3374 3838 4081 4093 1186 Size: 1044 937 54 153 464 243 12 4027 Fragments arranged by size: 4027 1044 937 464 243 153 54 12 BseYI C'CCAG_C Cuts at: 2104 3113 3569 6137 6633 2104 Size: 1009 456 2568 496 2405 Fragments arranged by size: 2568 2405 1009 496 456 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3476 3548 1163 Size: 573 1740 72 4549 Fragments arranged by size: 4549 1740 573 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2220 3471 3612 3843 4002 4384 Size: 275 186 219 1251 141 231 159 382 Cuts at: 4384 5145 6173 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1251 1028 761 382 275 231 219 186 159 141 BsiHKAI G_wGCw'C Cuts at: 2322 3876 4995 5080 6151 2322 Size: 1554 1119 85 1071 3105 Fragments arranged by size: 3105 1554 1119 1071 85 BsmI GAATG_Cn' Cuts at: 4445 4538 4445 Size: 93 6841 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2289 2322 2366 3213 3374 3389 Size: 399 463 36 33 44 847 161 15 Cuts at: 3389 3494 3552 3876 4011 4073 4253 4995 5080 Size: 105 58 324 135 62 180 742 85 Cuts at: 5080 6151 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 847 742 463 399 324 180 161 135 105 85 62 58 44 36 33 15 BspEI T'CCGG_A Cuts at: 6597 6597 Size: 6934 BspHI T'CATG_A Cuts at: 4823 6553 4823 Size: 1730 5204 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4289 844 Size: 3445 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3265 3448 3556 4089 4821 1394 Size: 429 1442 183 108 533 732 3507 Fragments arranged by size: 3507 1442 732 533 429 183 108 BsrDI GCAATG_nn' Cuts at: 65 1248 5429 5603 65 Size: 1183 4181 174 1396 Fragments arranged by size: 4181 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2225 3394 3656 3946 5578 5765 1700 Size: 110 415 1169 262 290 1632 187 2869 Fragments arranged by size: 2869 1632 1169 415 290 262 187 110 BsrGI T'GTAC_A Cuts at: 96 2556 96 Size: 2460 4474 BssHII G'CGCG_C Cuts at: 1925 3413 3415 3417 1925 Size: 1488 2 2 5442 Fragments arranged by size: 5442 1488 2 2 BssSI C'ACGA_G Cuts at: 4994 6006 4994 Size: 1012 5922 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6934 BstBI TT'CG_AA Cuts at: 5738 5738 Size: 6934 BstEII G'GTnAC_C Cuts at: 1184 2108 1184 Size: 924 6010 BstXI CCAn_nnnn'nTGG Cuts at: 3685 3685 Size: 6934 BstYI r'GATC_y Cuts at: 1972 2023 3186 4264 4631 5016 5033 6485 6571 Size: 51 1163 1078 367 385 17 1452 86 Cuts at: 6571 6583 6929 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1163 1078 385 367 346 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 2280 3234 3582 3636 3678 3684 3726 Size: 552 1215 954 348 54 42 6 42 Cuts at: 3726 3911 3990 4002 513 Size: 185 79 12 3445 Fragments arranged by size: 3445 1215 954 552 348 185 79 54 42 42 12 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2824 487 Size: 683 56 1598 4597 Fragments arranged by size: 4597 1598 683 56 BtsI GCAGTG_nn' Cuts at: 4065 4450 5244 5264 4065 Size: 385 794 20 5735 Fragments arranged by size: 5735 794 385 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6934 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6899 DraI TTT'AAA Cuts at: 2413 4238 2413 Size: 1825 5109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4022 5941 809 Size: 507 2706 1919 1802 Fragments arranged by size: 2706 1919 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2184 2217 2929 Size: 54 1425 50 275 18 354 33 712 Cuts at: 2929 3180 3396 3468 3609 3633 3681 3729 3780 Size: 251 216 72 141 24 48 48 51 Cuts at: 3780 3828 3840 3999 4035 4152 4381 5270 6899 Size: 48 12 159 36 117 229 889 1629 Cuts at: 6899 8 Size: 43 Fragments arranged by size: 1629 1425 889 712 354 275 251 229 216 159 141 117 72 54 51 50 48 48 48 43 36 33 24 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2217 3468 3609 3840 3999 4381 1537 Size: 275 405 1251 141 231 159 382 4090 Fragments arranged by size: 4090 1251 405 382 275 231 159 141 EarI CTCTTCn'nnn_ Cuts at: 1353 2289 2929 3402 3563 4864 1353 Size: 936 640 473 161 1301 3423 Fragments arranged by size: 3423 1301 936 640 473 161 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5521 5895 6041 387 Size: 1064 138 3932 374 146 1280 Fragments arranged by size: 3932 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 3007 3051 3583 3734 3847 4090 Size: 393 713 662 44 532 151 113 243 Cuts at: 4090 4997 5594 6381 6649 1239 Size: 907 597 787 268 1524 Fragments arranged by size: 1524 907 787 713 662 597 532 393 268 243 151 113 44 EcoNI CCTnn'n_nnAGG Cuts at: 3485 3485 Size: 6934 EcoO109I rG'GnC_Cy Cuts at: 2249 2250 3006 3490 3491 3623 3648 3775 4007 Size: 1 756 484 1 132 25 127 232 Cuts at: 4007 4008 4215 2249 Size: 1 207 4968 Fragments arranged by size: 4968 756 484 232 207 132 127 25 1 1 1 EcoRI G'AATT_C Cuts at: 2834 4243 4621 2834 Size: 1409 378 5147 Fragments arranged by size: 5147 1409 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3454 3454 Size: 6934 FspI TGC'GCA Cuts at: 5440 5440 Size: 6934 HaeII r_GCGC'y Cuts at: 1678 2081 2165 2181 3395 3594 6081 1678 Size: 403 84 16 1214 199 2487 2531 Fragments arranged by size: 2531 2487 1214 403 199 84 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 2128 2160 2185 Size: 32 135 32 441 32 83 32 25 Cuts at: 2185 2217 3005 3037 3281 3313 4055 4087 5575 Size: 32 788 32 244 32 742 32 1488 Cuts at: 5575 5607 5649 5681 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1488 788 742 441 244 135 83 42 32 32 32 32 32 32 32 32 32 32 25 HincII GTy'rAC Cuts at: 669 1657 1938 4278 4524 669 Size: 988 281 2340 246 3079 Fragments arranged by size: 3079 2340 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6934 HpaI GTT'AAC Cuts at: 4524 4524 Size: 6934 KasI G'GCGC_C Cuts at: 2077 2177 3590 2077 Size: 100 1413 5421 Fragments arranged by size: 5421 1413 100 KpnI G_GTAC'C Cuts at: 4259 4259 Size: 6934 MfeI C'AATT_G Cuts at: 2204 4533 2204 Size: 2329 4605 MluI A'CGCG_T Cuts at: 6810 6810 Size: 6934 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3183 6048 6232 1305 Size: 1878 2865 184 2007 Fragments arranged by size: 2865 2007 1878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2186 3635 3782 10 Size: 54 1425 343 354 1449 147 3162 Fragments arranged by size: 3162 1449 1425 354 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2369 2843 2972 3683 3824 3941 4892 Size: 648 1203 474 129 711 141 117 951 Cuts at: 4892 5251 5410 518 Size: 359 159 2042 Fragments arranged by size: 2042 1203 951 711 648 474 359 159 141 129 117 MspA1I CmG'CkG Cuts at: 1606 3236 3279 3614 3698 3728 3836 3913 4004 Size: 1630 43 335 84 30 108 77 91 Cuts at: 4004 4180 4350 5027 6175 6420 1606 Size: 176 170 677 1148 245 2120 Fragments arranged by size: 2120 1630 1148 677 335 245 176 170 108 91 84 77 43 30 NaeI GCC'GGC Cuts at: 3396 3396 Size: 6934 NarI GG'CG_CC Cuts at: 2078 2178 3591 2078 Size: 100 1413 5421 Fragments arranged by size: 5421 1413 100 NcoI C'CATG_G Cuts at: 513 1065 2280 3582 3684 3990 513 Size: 552 1215 1302 102 306 3457 Fragments arranged by size: 3457 1302 1215 552 306 102 NdeI CA'TA_TG Cuts at: 387 387 Size: 6934 NgoMIV G'CCGG_C Cuts at: 3394 3394 Size: 6934 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6934 NotI GC'GGCC_GC Cuts at: 3468 3609 3840 3999 4381 3468 Size: 141 231 159 382 6021 Fragments arranged by size: 6021 382 231 159 141 NruI TCG'CGA Cuts at: 3381 3381 Size: 6934 NspI r_CATG'y Cuts at: 1132 1229 4292 5837 1132 Size: 97 3063 1545 2229 Fragments arranged by size: 3063 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2245 3487 1348 Size: 897 1242 4795 Fragments arranged by size: 4795 1242 897 PciI A'CATG_T Cuts at: 5833 5833 Size: 6934 PflMI CCAn_nnn'nTGG Cuts at: 1108 4183 1108 Size: 3075 3859 PfoI T'CCnGG_A Cuts at: 2913 3951 2913 Size: 1038 5896 PmeI GTTT'AAAC Cuts at: 4238 4238 Size: 6934 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5003 5035 6516 6548 5003 Size: 32 1481 32 5389 Fragments arranged by size: 5389 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3006 3623 4215 3006 Size: 617 592 5725 Fragments arranged by size: 5725 617 592 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6934 PsiI TTA'TAA Cuts at: 2493 4504 2493 Size: 2011 4923 PspOMI G'GGCC_C Cuts at: 2249 3490 4007 2249 Size: 1241 517 5176 Fragments arranged by size: 5176 1241 517 PstI C_TGCA'G Cuts at: 830 2964 4170 4286 830 Size: 2134 1206 116 3478 Fragments arranged by size: 3478 2134 1206 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6934 SacII CC_GC'GG Cuts at: 3237 3729 3914 4005 3237 Size: 492 185 91 6166 Fragments arranged by size: 6166 492 185 91 SalI G'TCGA_C Cuts at: 1936 4276 1936 Size: 2340 4594 SanDI GG'GwC_CC Cuts at: 3623 3623 Size: 6934 SapI GCTCTTCn'nnn_ Cuts at: 3563 3563 Size: 6934 SbfI CC_TGCA'GG Cuts at: 4286 4286 Size: 6934 ScaI AGT'ACT Cuts at: 5182 6894 5182 Size: 1712 5222 SfcI C'TryA_G Cuts at: 826 1045 2960 3081 3922 4166 4282 5417 6098 Size: 219 1915 121 841 244 116 1135 681 Cuts at: 6098 6289 826 Size: 191 1471 Fragments arranged by size: 1915 1471 1135 841 681 244 219 191 121 116 SfiI GGCCn_nnn'nGGCC Cuts at: 3528 3528 Size: 6934 SfoI GGC'GCC Cuts at: 2079 2179 3592 2079 Size: 100 1413 5421 Fragments arranged by size: 5421 1413 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6934 SgrAI Cr'CCGG_yG Cuts at: 1700 3656 1700 Size: 1956 4978 SmaI CCC'GGG Cuts at: 1872 3272 3426 3519 4261 1872 Size: 1400 154 93 742 4545 Fragments arranged by size: 4545 1400 742 154 93 SmlI C'TyrA_G Cuts at: 820 1017 1943 5038 5939 6201 820 Size: 197 926 3095 901 262 1553 Fragments arranged by size: 3095 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4725 493 Size: 4232 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6934 SphI G_CATG'C Cuts at: 1132 4292 1132 Size: 3160 3774 SspI AAT'ATT Cuts at: 5 52 4858 5 Size: 47 4806 2081 Fragments arranged by size: 4806 2081 47 StuI AGG'CCT Cuts at: 1517 2269 3170 3437 1517 Size: 752 901 267 5014 Fragments arranged by size: 5014 901 752 267 StyI C'CwwG_G Cuts at: 513 1065 2280 2313 3399 3582 3684 3990 4373 Size: 552 1215 33 1086 183 102 306 383 Cuts at: 4373 513 Size: 3074 Fragments arranged by size: 3074 1215 1086 552 383 306 183 102 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4961 4978 5131 5316 5761 325 Size: 1255 3381 17 153 185 445 1498 Fragments arranged by size: 3381 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2556 2801 5180 Size: 274 80 33 51 1443 579 245 2379 Cuts at: 5180 6892 96 Size: 1712 138 Fragments arranged by size: 2379 1712 1443 579 274 245 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6894 6926 6894 Size: 32 6902 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6934 XbaI T'CTAG_A Cuts at: 4270 4270 Size: 6934 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2194 2320 2194 Size: 126 6808 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6934 XmaI C'CCGG_G Cuts at: 1870 3270 3424 3517 4259 1870 Size: 1400 154 93 742 4545 Fragments arranged by size: 4545 1400 742 154 93 XmnI GAAnn'nnTTC Cuts at: 5063 5063 Size: 6934 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4742 276 Size: 53 83 186 936 3208 2468 Fragments arranged by size: 3208 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SexAI SrfI SwaI