################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BalI BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BpmI BsaAI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI HhaI HinP1I HpaI KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AcsI 52 AAATTC [GA]^AATT[CT] AfaI 967 GTAC GT^AC AocI 1060 CCTAAGG CC^T[ACGT]AGG ApaI 462 GGGCCC GGGCC^C ApoI 52 AAATTC [GA]^AATT[CT] Asp700I 48 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 940 GAGCTC G[AT]GC[AT]^C AvaI 295 CCCGAG C^[CT]CG[GA]G AvrII 772 CCTAGG C^CTAGG BanI 523 GGTGCC G^G[CT][GA]CC BbvCI 533 CCTCAGC CCTCAGC(-5/-2) BglI 226 GCCATCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 772 CCTAGG C^CTAGG BlpI 937 GCTGAGC GC^T[ACGT]AGC Bpu1102I 937 GCTGAGC GC^T[ACGT]AGC BsiHKAI 940 GAGCTC G[AT]GC[AT]^C BsoBI 295 CCCGAG C^[CT]CG[GA]G Bsp1407I 966 TGTACA T^GTACA BsrDI 83 CATTGC GCAATG(2/0) BsrGI 966 TGTACA T^GTACA Bsu36I 1060 CCTAAGG CC^T[ACGT]AGG CelII 937 GCTGAGC GC^T[ACGT]AGC Eco81I 1060 CCTAAGG CC^T[ACGT]AGG EcoNI 772 CCTAGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 979 GTCAAC GT[CT]^[GA]AC HindII 979 GTCAAC GT[CT]^[GA]AC HindIII 492 AAGCTT A^AGCTT MaeIII 898 GTCAC ^GT[ACGT]AC MseI 286 TTAA T^TAA MspA1I 199 CAGCTG C[AC]G^C[GT]G NcoI 869 CCATGG C^CATGG PflMI 466 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 43 CTGCAG CTGCA^G PvuII 199 CAGCTG CAG^CTG RsaI 967 GTAC GT^AC SacI 940 GAGCTC GAGCT^C SfaNI 1009 GATGC GCATC(5/9) SspBI 966 TGTACA T^GTACA TfiI 748 GATTC G^A[AT]TC Tru9I 286 TTAA T^TAA Tsp45I 898 GTCAC ^GT[GC]AC Tsp509I 53 AATT ^AATT Van91I 466 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 48 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 374 GACGTC GACGT^C 1108 GACGTC AccI 1096 GTCTAC GT^[AC][GT]AC 1111 GTCTAC AcyI 374 GACGTC G[GA]^CG[CT]C 1108 GACGTC AlwNI 467 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 882 CAGAAACTG BbsI 805 GAAGAC GAAGAC(2/6) 411 GTCTTC BpuAI 805 GAAGAC GAAGAC(2/6) 411 GTCTTC BsaHI 374 GACGTC G[GA]^CG[CT]C 1108 GACGTC BspMI 356 ACCTGC ACCTGC(4/8) 740 GCAGGT BstNI 466 CCAGG CC^[AT]GG 954 CCAGG DsaI 828 CCGTGG C^C[GA][CT]GG 869 CCATGG EarI 31 GAAGAG CTCTTC(1/4) 451 GAAGAG EcoRII 466 CCAGG ^CC[AT]GG 954 CCAGG Hin1I 374 GACGTC G[GA]^CG[CT]C 1108 GACGTC Ksp632I 31 GAAGAG CTCTTC(1/4) 451 GAAGAG MaeII 375 ACGT A^CGT 1109 ACGT MvaI 466 CCAGG CC^[AT]GG 954 CCAGG SfcI 43 CTGCAG C^T[GA][CT]AG 1098 CTACAG SmlI 64 CTCAAG C^T[CT][GA]AG 274 CTTGAG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BfaI BmyI BsaBI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MamI MboI MboII MnlI MspI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI Sau3AI Sau96I ScrFI StyI TaqI TseI TspRI TthHB8I