(Circular) (Six-base) MAPSORT of: pFN21AE4361.seq Check: 1974 from: 1 to: 5845 pFN21AE4361 5845 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2383 3117 3655 278 Size: 53 83 186 936 847 734 538 2468 Fragments arranged by size: 2468 936 847 734 538 186 83 53 AccI GT'mk_AC Cuts at: 1937 3102 3117 3188 1937 Size: 1165 15 71 4594 Fragments arranged by size: 4594 1165 71 15 Acc65I G'GTAC_C Cuts at: 3166 3166 Size: 5845 AclI AA'CG_TT Cuts at: 1563 3972 4345 1563 Size: 2409 373 3063 Fragments arranged by size: 3063 2409 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2120 3908 5292 5560 1632 Size: 488 1788 1384 268 1917 Fragments arranged by size: 1917 1788 1384 488 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5648 AflIII A'CryG_T Cuts at: 1203 4744 5721 1203 Size: 3541 977 1327 Fragments arranged by size: 3541 1327 977 AgeI A'CCGG_T Cuts at: 4676 4676 Size: 5845 AhdI GACnn_n'nnGTC Cuts at: 4574 4574 Size: 5845 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5845 AlwNI CAG_nnn'CTG Cuts at: 2477 2892 3588 5160 2477 Size: 415 696 1572 3162 Fragments arranged by size: 3162 1572 696 415 ApaI G_GGCC'C Cuts at: 2471 2471 Size: 5845 ApaLI G'TGCA_C Cuts at: 3902 5058 3902 Size: 1156 4689 ApoI r'AATT_y Cuts at: 1493 2057 3154 3380 3532 5641 1493 Size: 564 1097 226 152 2109 1697 Fragments arranged by size: 2109 1697 1097 564 226 152 AseI AT'TA_AT Cuts at: 160 1032 4399 160 Size: 872 3367 1606 Fragments arranged by size: 3367 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 AvaI C'yCGr_G Cuts at: 1870 1943 2300 3170 1870 Size: 73 357 870 4545 Fragments arranged by size: 4545 870 357 73 AvrII C'CTAG_G Cuts at: 2777 2777 Size: 5845 BamHI G'GATC_C Cuts at: 3175 3542 3175 Size: 367 5478 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2528 3166 4621 618 Size: 325 217 627 741 638 1455 1842 Fragments arranged by size: 1842 1455 741 638 627 325 217 BanII G_rGCy'C Cuts at: 1391 2471 2776 2949 3044 3164 1391 Size: 1080 305 173 95 120 4072 Fragments arranged by size: 4072 1080 305 173 120 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 2817 928 Size: 971 510 408 3956 Fragments arranged by size: 3956 971 510 408 BbvCI CC'TCA_GC Cuts at: 2539 2539 Size: 5845 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4036 4070 1903 Size: 34 2099 34 3678 Fragments arranged by size: 3678 2099 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3737 4947 3737 Size: 1210 4635 BclI T'GATC_A Cuts at: 1548 1548 Size: 5845 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2237 4456 136 Size: 107 122 71 631 605 565 2219 1525 Fragments arranged by size: 2219 1525 631 605 565 122 107 71 BglII A'GATC_T Cuts at: 5840 5840 Size: 5845 BlpI GC'TnA_GC Cuts at: 2943 3262 2943 Size: 319 5526 Bme1580I G_kGCm'C Cuts at: 1790 2471 2533 3906 5062 1790 Size: 681 62 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 681 62 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5845 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2515 3576 4534 Size: 416 53 83 399 24 1098 1061 958 Cuts at: 4534 442 Size: 1753 Fragments arranged by size: 1753 1098 1061 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5845 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2412 2412 Size: 5845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4505 1239 Size: 3266 2579 Bpu10I CC'TnA_GC Cuts at: 2539 2539 Size: 5845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2054 2300 3970 4835 5133 2054 Size: 246 1670 865 298 2766 Fragments arranged by size: 2766 1670 865 298 246 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 2592 2616 2676 2712 2718 4508 Size: 617 738 355 24 60 36 6 1790 Cuts at: 4508 882 Size: 2219 Fragments arranged by size: 2219 1790 738 617 355 60 36 24 6 BsaAI yAC'GTr Cuts at: 493 1204 3636 493 Size: 711 2432 2702 Fragments arranged by size: 2702 2432 711 BsaBI GATnn'nnATC Cuts at: 2664 2670 2676 5839 2664 Size: 6 6 3163 2670 Fragments arranged by size: 3163 2670 6 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2380 3114 3652 4034 Size: 53 83 186 936 847 734 538 382 Cuts at: 4034 4669 275 Size: 635 1451 Fragments arranged by size: 1451 936 847 734 635 538 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4278 4676 4950 5097 5508 4278 Size: 398 274 147 411 4615 Fragments arranged by size: 4615 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5620 Fragments arranged by size: 5620 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2353 2527 1186 Size: 868 299 174 4504 Fragments arranged by size: 4504 868 299 174 BseYI C'CCAG_C Cuts at: 5048 5544 5048 Size: 496 5349 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5272 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3295 4056 5084 1540 Size: 275 186 1294 761 1028 2301 Fragments arranged by size: 2301 1294 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2949 3906 3991 5062 2949 Size: 957 85 1071 3732 Fragments arranged by size: 3732 1071 957 85 BsmI GAATG_Cn' Cuts at: 3356 3449 3356 Size: 93 5752 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2471 2533 2776 2949 3044 3164 3906 Size: 399 681 62 243 173 95 120 742 Cuts at: 3906 3991 5062 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 681 399 243 173 120 95 85 62 BspEI T'CCGG_A Cuts at: 5508 5508 Size: 5845 BspHI T'CATG_A Cuts at: 3734 5464 3734 Size: 1730 4115 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2370 2736 3200 844 Size: 1526 366 464 3489 Fragments arranged by size: 3489 1526 464 366 BsrBI CCG'CTC Cuts at: 1394 1823 2614 2650 2710 3732 1394 Size: 429 791 36 60 1022 3507 Fragments arranged by size: 3507 1022 791 429 60 36 BsrDI GCAATG_nn' Cuts at: 65 1248 2087 4340 4514 65 Size: 1183 839 2253 174 1396 Fragments arranged by size: 2253 1396 1183 839 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4489 4676 1700 Size: 110 2679 187 2869 Fragments arranged by size: 2869 2679 187 110 BsrGI T'GTAC_A Cuts at: 96 2971 96 Size: 2875 2970 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5845 BssSI C'ACGA_G Cuts at: 3905 4917 3905 Size: 1012 4833 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5845 BstBI TT'CG_AA Cuts at: 4649 4649 Size: 5845 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5845 BstYI r'GATC_y Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840 Size: 1203 367 385 17 1452 86 12 346 Cuts at: 5840 1972 Size: 1977 Fragments arranged by size: 1977 1452 1203 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3066 3066 Size: 5845 BtgI C'CryG_G Cuts at: 513 1065 2833 2874 513 Size: 552 1768 41 3484 Fragments arranged by size: 3484 1768 552 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5106 Fragments arranged by size: 5106 683 56 BtsI GCAGTG_nn' Cuts at: 2530 3361 4155 4175 2530 Size: 831 794 20 4200 Fragments arranged by size: 4200 831 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5845 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5810 DraI TTT'AAA Cuts at: 3149 3149 Size: 5845 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4852 809 Size: 507 3536 1802 Fragments arranged by size: 3536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3292 4181 5810 Size: 54 1425 50 275 18 1462 889 1629 Cuts at: 5810 8 Size: 43 Fragments arranged by size: 1629 1462 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3292 1537 Size: 275 1480 4090 Fragments arranged by size: 4090 1480 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2451 3775 1353 Size: 678 420 1324 3423 Fragments arranged by size: 3423 1324 678 420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4432 4806 4952 387 Size: 1064 138 2843 374 146 1280 Fragments arranged by size: 2843 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2947 2947 Size: 5845 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2120 3908 4505 5292 5560 1239 Size: 393 488 1788 597 787 268 1524 Fragments arranged by size: 1788 1524 787 597 488 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2781 2781 Size: 5845 EcoO109I rG'GnC_Cy Cuts at: 2296 2467 2735 2838 2955 3090 2296 Size: 171 268 103 117 135 5051 Fragments arranged by size: 5051 268 171 135 117 103 EcoRI G'AATT_C Cuts at: 3154 3532 3154 Size: 378 5467 FspI TGC'GCA Cuts at: 4351 4351 Size: 5845 HaeII r_GCGC'y Cuts at: 1678 4992 1678 Size: 3314 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373 Size: 32 135 32 2914 32 42 32 2626 Fragments arranged by size: 2914 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3189 3435 669 Size: 988 281 1048 203 246 3079 Fragments arranged by size: 3079 1048 988 281 246 203 HindIII A'AGCT_T Cuts at: 748 2497 748 Size: 1749 4096 HpaI GTT'AAC Cuts at: 3435 3435 Size: 5845 KpnI G_GTAC'C Cuts at: 3170 3170 Size: 5845 MfeI C'AATT_G Cuts at: 3444 3444 Size: 5845 MluI A'CGCG_T Cuts at: 5721 5721 Size: 5845 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2730 4959 5143 1305 Size: 1425 2229 184 2007 Fragments arranged by size: 2229 2007 1425 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4023 Fragments arranged by size: 4023 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3803 4162 4321 518 Size: 648 843 1794 359 159 2042 Fragments arranged by size: 2042 1794 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2206 3261 3938 5086 5331 1606 Size: 600 1055 677 1148 245 2120 Fragments arranged by size: 2120 1148 1055 677 600 245 NcoI C'CATG_G Cuts at: 513 1065 2874 513 Size: 552 1809 3484 Fragments arranged by size: 3484 1809 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5845 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5845 NotI GC'GGCC_GC Cuts at: 3292 3292 Size: 5845 NspI r_CATG'y Cuts at: 1132 1229 3203 4748 1132 Size: 97 1974 1545 2229 Fragments arranged by size: 2229 1974 1545 97 PasI CC'CwG_GG Cuts at: 1348 2471 1348 Size: 1123 4722 PciI A'CATG_T Cuts at: 4744 4744 Size: 5845 PflMI CCAn_nnn'nTGG Cuts at: 1108 2477 1108 Size: 1369 4476 PmeI GTTT'AAAC Cuts at: 3149 3149 Size: 5845 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3914 3946 5427 5459 3914 Size: 32 1481 32 4300 Fragments arranged by size: 4300 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2296 2735 2955 3090 2296 Size: 439 220 135 5051 Fragments arranged by size: 5051 439 220 135 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5845 PsiI TTA'TAA Cuts at: 3415 3415 Size: 5845 PspOMI G'GGCC_C Cuts at: 2467 2467 Size: 5845 PstI C_TGCA'G Cuts at: 830 2052 3197 830 Size: 1222 1145 3478 Fragments arranged by size: 3478 1222 1145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5845 PvuII CAG'CTG Cuts at: 2206 2206 Size: 5845 SacI G_AGCT'C Cuts at: 2949 2949 Size: 5845 SalI G'TCGA_C Cuts at: 1936 3187 1936 Size: 1251 4594 SanDI GG'GwC_CC Cuts at: 2955 2955 Size: 5845 SbfI CC_TGCA'GG Cuts at: 3197 3197 Size: 5845 ScaI AGT'ACT Cuts at: 4093 5805 4093 Size: 1712 4133 SfcI C'TryA_G Cuts at: 826 1045 2048 3103 3193 4328 5009 5200 826 Size: 219 1003 1055 90 1135 681 191 1471 Fragments arranged by size: 1471 1135 1055 1003 681 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5845 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5845 SmaI CCC'GGG Cuts at: 1872 3172 1872 Size: 1300 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2279 3949 4850 5112 820 Size: 197 926 126 210 1670 901 262 1553 Fragments arranged by size: 1670 1553 926 901 262 210 197 126 SnaBI TAC'GTA Cuts at: 493 3636 493 Size: 3143 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5845 SphI G_CATG'C Cuts at: 1132 3203 1132 Size: 2071 3774 SspI AAT'ATT Cuts at: 5 52 3769 5 Size: 47 3717 2081 Fragments arranged by size: 3717 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5845 StyI C'CwwG_G Cuts at: 513 1065 2131 2777 2874 3284 513 Size: 552 1066 646 97 410 3074 Fragments arranged by size: 3074 1066 646 552 410 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2616 2736 2742 2836 2899 3872 3889 Size: 1255 1036 120 6 94 63 973 17 Cuts at: 3889 4042 4227 4672 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1036 973 445 185 153 120 94 63 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2971 4091 5803 Size: 274 80 33 51 1443 994 1120 1712 Cuts at: 5803 96 Size: 138 Fragments arranged by size: 1712 1443 1120 994 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5805 5837 5805 Size: 32 5813 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5845 XbaI T'CTAG_A Cuts at: 3181 3181 Size: 5845 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5845 XmaI C'CCGG_G Cuts at: 1870 3170 1870 Size: 1300 4545 XmnI GAAnn'nnTTC Cuts at: 2057 3974 2057 Size: 1917 3928 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2381 3115 3653 276 Size: 53 83 186 936 847 734 538 2468 Fragments arranged by size: 2468 936 847 734 538 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI