(Linear) (Six-base) MAPSORT of: KIEE4361.seq Check: 6455 from: 1 to: 1140 KIEE4361.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. AatII G_ACGT'C Cuts at: 0 378 1112 1140 Size: 378 734 28 Fragments arranged by size: 734 378 28 AccI GT'mk_AC Cuts at: 0 1097 1112 1140 Size: 1097 15 28 Fragments arranged by size: 1097 28 15 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 1140 Size: 115 1025 AlwNI CAG_nnn'CTG Cuts at: 0 472 887 1140 Size: 472 415 253 Fragments arranged by size: 472 415 253 ApaI G_GGCC'C Cuts at: 0 466 1140 Size: 466 674 ApoI r'AATT_y Cuts at: 0 52 1140 Size: 52 1088 AvaI C'yCGr_G Cuts at: 0 295 1140 Size: 295 845 AvrII C'CTAG_G Cuts at: 0 772 1140 Size: 772 368 BanI G'GyrC_C Cuts at: 0 523 1140 Size: 523 617 BanII G_rGCy'C Cuts at: 0 466 771 944 1039 1140 Size: 466 305 173 95 101 Fragments arranged by size: 466 305 173 101 95 BbsI GAAGACnn'nnnn_ Cuts at: 0 404 812 1140 Size: 404 408 328 Fragments arranged by size: 408 404 328 BbvCI CC'TCA_GC Cuts at: 0 534 1140 Size: 534 606 BglI GCCn_nnn'nGGC Cuts at: 0 232 1140 Size: 232 908 BlpI GC'TnA_GC Cuts at: 0 938 1140 Size: 938 202 Bme1580I G_kGCm'C Cuts at: 0 466 528 1140 Size: 466 62 612 Fragments arranged by size: 612 466 62 BmrI ACTGGGnnnn_n' Cuts at: 0 510 1140 Size: 510 630 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 407 1140 Size: 407 733 Bpu10I CC'TnA_GC Cuts at: 0 534 1140 Size: 534 606 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 49 295 1140 Size: 49 246 845 Fragments arranged by size: 845 246 49 BsaI GGTCTCn'nnnn_ Cuts at: 0 232 587 611 671 707 713 1140 Size: 232 355 24 60 36 6 427 Fragments arranged by size: 427 355 232 60 36 24 6 BsaBI GATnn'nnATC Cuts at: 0 659 665 671 1140 Size: 659 6 6 469 Fragments arranged by size: 659 469 6 6 BsaHI Gr'CG_yC Cuts at: 0 375 1109 1140 Size: 375 734 31 Fragments arranged by size: 734 375 31 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 49 348 522 1140 Size: 49 299 174 618 Fragments arranged by size: 618 299 174 49 BsiHKAI G_wGCw'C Cuts at: 0 944 1140 Size: 944 196 Bsp1286I G_dGCh'C Cuts at: 0 466 528 771 944 1039 1140 Size: 466 62 243 173 95 101 Fragments arranged by size: 466 243 173 101 95 62 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 365 731 1140 Size: 365 366 409 Fragments arranged by size: 409 366 365 BsrBI CCG'CTC Cuts at: 0 609 645 705 1140 Size: 609 36 60 435 Fragments arranged by size: 609 435 60 36 BsrDI GCAATG_nn' Cuts at: 0 82 1140 Size: 82 1058 BsrGI T'GTAC_A Cuts at: 0 966 1140 Size: 966 174 Bsu36I CC'TnA_GG Cuts at: 0 1061 1140 Size: 1061 79 BtgI C'CryG_G Cuts at: 0 828 869 1140 Size: 828 41 271 Fragments arranged by size: 828 271 41 BtsI GCAGTG_nn' Cuts at: 0 525 1140 Size: 525 615 EarI CTCTTCn'nnn_ Cuts at: 0 26 446 1140 Size: 26 420 694 Fragments arranged by size: 694 420 26 EcoICRI GAG'CTC Cuts at: 0 942 1140 Size: 942 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 1140 Size: 115 1025 EcoNI CCTnn'n_nnAGG Cuts at: 0 776 1140 Size: 776 364 EcoO109I rG'GnC_Cy Cuts at: 0 291 462 730 833 950 1085 1140 Size: 291 171 268 103 117 135 55 Fragments arranged by size: 291 268 171 135 117 103 55 HincII GTy'rAC Cuts at: 0 981 1140 Size: 981 159 HindIII A'AGCT_T Cuts at: 0 492 1140 Size: 492 648 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 725 1140 Size: 725 415 MspA1I CmG'CkG Cuts at: 0 201 1140 Size: 201 939 NcoI C'CATG_G Cuts at: 0 869 1140 Size: 869 271 PasI CC'CwG_GG Cuts at: 0 466 1140 Size: 466 674 PflMI CCAn_nnn'nTGG Cuts at: 0 472 1140 Size: 472 668 PpuMI rG'GwC_Cy Cuts at: 0 291 730 950 1085 1140 Size: 291 439 220 135 55 Fragments arranged by size: 439 291 220 135 55 PspOMI G'GGCC_C Cuts at: 0 462 1140 Size: 462 678 PstI C_TGCA'G Cuts at: 0 47 1140 Size: 47 1093 PvuII CAG'CTG Cuts at: 0 201 1140 Size: 201 939 SacI G_AGCT'C Cuts at: 0 944 1140 Size: 944 196 SanDI GG'GwC_CC Cuts at: 0 950 1140 Size: 950 190 SfcI C'TryA_G Cuts at: 0 43 1098 1140 Size: 43 1055 42 Fragments arranged by size: 1055 43 42 SmlI C'TyrA_G Cuts at: 0 64 274 1140 Size: 64 210 866 Fragments arranged by size: 866 210 64 StyI C'CwwG_G Cuts at: 0 126 772 869 1140 Size: 126 646 97 271 Fragments arranged by size: 646 271 126 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 611 731 737 831 894 1140 Size: 611 120 6 94 63 246 Fragments arranged by size: 611 246 120 94 63 6 TatI w'GTAC_w Cuts at: 0 966 1140 Size: 966 174 XmnI GAAnn'nnTTC Cuts at: 0 52 1140 Size: 52 1088 ZraI GAC'GTC Cuts at: 0 376 1110 1140 Size: 376 734 30 Fragments arranged by size: 734 376 30 Enzymes that do cut: AatII AccI AcuI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaBI BsaHI BseRI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrGI Bsu36I BtgI BtsI EarI EcoICRI Eco57MI EcoNI EcoO109I HincII HindIII MmeI MspA1I NcoI PasI PflMI PpuMI PspOMI PstI PvuII SacI SanDI SfcI SmlI StyI TaqII TatI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BpmI BsaAI BsaWI BsaXI BseYI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI