(Circular) (Six-base) MAPSORT of: pFN21AE1344.seq Check: 4416 from: 1 to: 6385 pFN21AE1344 6385 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3484 3484 Size: 6385 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3728 1937 Size: 1791 4594 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2355 2444 3365 4448 5832 6100 1632 Size: 723 89 921 1083 1384 268 1917 Fragments arranged by size: 1917 1384 1083 921 723 268 89 AflII C'TTAA_G Cuts at: 820 1017 2276 2638 820 Size: 197 1259 362 4567 Fragments arranged by size: 4567 1259 362 197 AflIII A'CryG_T Cuts at: 1203 5284 6261 1203 Size: 4081 977 1327 Fragments arranged by size: 4081 1327 977 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 2574 1166 Size: 1408 4977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 3106 4128 5700 3106 Size: 1022 1572 3791 Fragments arranged by size: 3791 1572 1022 ApaI G_GGCC'C Cuts at: 2834 2834 Size: 6385 ApaLI G'TGCA_C Cuts at: 4442 5598 4442 Size: 1156 5229 ApoI r'AATT_y Cuts at: 1493 2401 2554 2979 3694 3920 4072 6181 1493 Size: 908 153 425 715 226 152 2109 1697 Fragments arranged by size: 2109 1697 908 715 425 226 153 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3710 1870 Size: 73 121 1646 4545 Fragments arranged by size: 4545 1646 121 73 AvrII C'CTAG_G Cuts at: 2987 2987 Size: 6385 BamHI G'GATC_C Cuts at: 3715 4082 3715 Size: 367 6018 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3706 5161 618 Size: 325 217 627 1919 1455 1842 Fragments arranged by size: 1919 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2834 3540 3704 1391 Size: 1443 706 164 4072 Fragments arranged by size: 4072 1443 706 164 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5414 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4576 4610 1903 Size: 34 50 34 2555 34 3678 Fragments arranged by size: 3678 2555 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2779 4277 5487 2779 Size: 1498 1210 3677 Fragments arranged by size: 3677 1498 1210 BclI T'GATC_A Cuts at: 1548 3417 1548 Size: 1869 4516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4996 136 Size: 107 122 71 631 605 3324 1525 Fragments arranged by size: 3324 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3390 6380 3390 Size: 2990 3395 BlpI GC'TnA_GC Cuts at: 2921 3802 2921 Size: 881 5504 Bme1580I G_kGCm'C Cuts at: 1790 2834 3136 4446 5602 1790 Size: 1044 302 1310 1156 2573 Fragments arranged by size: 2573 1310 1156 1044 302 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4116 5074 442 Size: 416 53 83 399 24 2699 958 1753 Fragments arranged by size: 2699 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6385 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2074 5045 1239 Size: 835 2971 2579 Fragments arranged by size: 2971 2579 835 Bpu10I CC'TnA_GC Cuts at: 2745 2745 Size: 6385 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2581 2645 2731 3367 4510 5375 5673 2581 Size: 64 86 636 1143 865 298 3293 Fragments arranged by size: 3293 1143 865 636 298 86 64 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5048 882 Size: 617 3549 2219 Fragments arranged by size: 3549 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4176 493 Size: 711 2972 2702 Fragments arranged by size: 2972 2702 711 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4192 4574 5209 275 Size: 53 83 186 936 2659 382 635 1451 Fragments arranged by size: 2659 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4818 5216 5490 5637 6048 4818 Size: 398 274 147 411 5155 Fragments arranged by size: 5155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2122 2152 1178 Size: 30 165 30 719 30 5411 Fragments arranged by size: 5411 719 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2130 1186 Size: 944 5441 BseYI C'CCAG_C Cuts at: 2734 2780 5588 6084 2734 Size: 46 2808 496 3035 Fragments arranged by size: 3035 2808 496 46 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5812 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3835 4596 5624 1540 Size: 275 186 1834 761 1028 2301 Fragments arranged by size: 2301 1834 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2614 3364 4446 4531 5602 2614 Size: 750 1082 85 1071 3397 Fragments arranged by size: 3397 1082 1071 750 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2614 2834 3136 3364 3540 3704 4446 Size: 399 824 220 302 228 176 164 742 Cuts at: 4446 4531 5602 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 824 742 399 302 228 220 176 164 85 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3484 3740 844 Size: 2640 256 3489 Fragments arranged by size: 3489 2640 256 BsrBI CCG'CTC Cuts at: 1394 1823 4272 1394 Size: 429 2449 3507 Fragments arranged by size: 3507 2449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4880 5054 65 Size: 1183 3632 174 1396 Fragments arranged by size: 3632 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5029 5216 1700 Size: 110 3219 187 2869 Fragments arranged by size: 3219 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2895 96 Size: 2799 3586 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 2142 4445 5457 2142 Size: 2303 1012 3070 Fragments arranged by size: 3070 2303 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6385 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstYI r'GATC_y Cuts at: 1972 2965 3390 3715 4082 4467 4484 5936 6022 Size: 993 425 325 367 385 17 1452 86 Cuts at: 6022 6034 6380 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 993 425 385 367 346 325 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2915 513 Size: 552 1850 3983 Fragments arranged by size: 3983 1850 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2199 487 Size: 683 56 973 4673 Fragments arranged by size: 4673 973 683 56 BtsI GCAGTG_nn' Cuts at: 3901 4695 4715 3901 Size: 794 20 5571 Fragments arranged by size: 5571 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3006 3041 529 Size: 35 2442 35 3873 Fragments arranged by size: 3873 2442 35 35 DraI TTT'AAA Cuts at: 2683 3689 2683 Size: 1006 5379 DraIII CAC_nnn'GTG Cuts at: 2938 2938 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2317 5392 809 Size: 507 1001 3075 1802 Fragments arranged by size: 3075 1802 1001 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2912 3832 4721 Size: 54 1425 50 275 18 1082 920 889 Cuts at: 4721 6350 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1082 920 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2757 3384 3609 4315 1353 Size: 1404 627 225 706 3423 Fragments arranged by size: 3423 1404 706 627 225 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4972 5346 5492 387 Size: 1064 138 3383 374 146 1280 Fragments arranged by size: 3383 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2074 2355 2444 3365 4448 5045 5832 Size: 393 442 281 89 921 1083 597 787 Cuts at: 5832 6100 1239 Size: 268 1524 Fragments arranged by size: 1524 1083 921 787 597 442 393 281 268 89 EcoNI CCTnn'n_nnAGG Cuts at: 2663 2663 Size: 6385 EcoO109I rG'GnC_Cy Cuts at: 2830 2831 2830 Size: 1 6384 EcoRI G'AATT_C Cuts at: 2979 3694 4072 2979 Size: 715 378 5292 Fragments arranged by size: 5292 715 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3095 3095 Size: 6385 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 5532 1678 Size: 3854 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3277 3309 3509 3541 5026 Size: 32 135 32 1705 32 200 32 1485 Cuts at: 5026 5058 5100 5132 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1705 1485 200 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3729 3975 669 Size: 988 281 1791 246 3079 Fragments arranged by size: 3079 1791 988 281 246 HindIII A'AGCT_T Cuts at: 748 3648 748 Size: 2900 3485 HpaI GTT'AAC Cuts at: 3975 3975 Size: 6385 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5499 5683 1305 Size: 4194 184 2007 Fragments arranged by size: 4194 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2914 10 Size: 54 1425 343 1082 3481 Fragments arranged by size: 3481 1425 1082 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 2840 4343 4702 4861 518 Size: 648 1408 266 1503 359 159 2042 Fragments arranged by size: 2042 1503 1408 648 359 266 159 MspA1I CmG'CkG Cuts at: 1606 2308 2504 2780 3022 3801 4478 5626 5871 Size: 702 196 276 242 779 677 1148 245 Cuts at: 5871 1606 Size: 2120 Fragments arranged by size: 2120 1148 779 702 677 276 245 242 196 NcoI C'CATG_G Cuts at: 513 1065 2915 513 Size: 552 1850 3983 Fragments arranged by size: 3983 1850 552 NdeI CA'TA_TG Cuts at: 387 2814 387 Size: 2427 3958 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6385 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NspI r_CATG'y Cuts at: 1132 1229 2442 2707 3635 3743 5288 1132 Size: 97 1213 265 928 108 1545 2229 Fragments arranged by size: 2229 1545 1213 928 265 108 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6385 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6385 PfoI T'CCnGG_A Cuts at: 2614 2614 Size: 6385 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3087 3119 4454 4486 5967 5999 3087 Size: 32 1335 32 1481 32 3473 Fragments arranged by size: 3473 1481 1335 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PspOMI G'GGCC_C Cuts at: 2830 2830 Size: 6385 PstI C_TGCA'G Cuts at: 830 2824 3121 3737 830 Size: 1994 297 616 3478 Fragments arranged by size: 3478 1994 616 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2308 2504 2780 3022 2308 Size: 196 276 242 5671 Fragments arranged by size: 5671 276 242 196 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2820 3117 3469 3733 4868 5549 5740 Size: 219 1775 297 352 264 1135 681 191 Cuts at: 5740 826 Size: 1471 Fragments arranged by size: 1775 1471 1135 681 352 297 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 3712 1872 Size: 1840 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2276 2560 2638 2660 2710 3346 Size: 197 926 333 284 78 22 50 636 Cuts at: 3346 4489 5390 5652 820 Size: 1143 901 262 1553 Fragments arranged by size: 1553 1143 926 901 636 333 284 262 197 78 50 22 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3743 1132 Size: 2611 3774 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6385 StyI C'CwwG_G Cuts at: 513 1065 2915 2987 3824 513 Size: 552 1850 72 837 3074 Fragments arranged by size: 3074 1850 837 552 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4412 4429 4582 4767 5212 325 Size: 1255 2832 17 153 185 445 1498 Fragments arranged by size: 2832 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 4631 6343 Size: 274 80 33 51 1443 918 1736 1712 Cuts at: 6343 96 Size: 138 Fragments arranged by size: 1736 1712 1443 918 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6345 6377 6345 Size: 32 6353 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3092 3721 3092 Size: 629 5756 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2842 2176 Size: 666 5719 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3710 1870 Size: 1840 4545 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI