Restriction map for
pFN21AB6792
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI AlwI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeIII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MamI MfeI MflI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tsp509I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AciI 306 GCGG CCGC(-3/-1)
Alw44I 363 GTGCAC G^TGCAC
AlwNI 196 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 363 GTGCAC G^TGCAC
BcgI 333 CGAGTCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsmI 426 GAATGC GAATGC(1/-1)
BsrFI 302 ACCGGC [GA]^CCGG[CT]
CfoI 16 GCGC GCG^C
Cfr10I 302 ACCGGC [GA]^CCGG[CT]
DdeI 7 CTGAG C^T[ACGT]AG
EarI 118 GAAGAG CTCTTC(1/4)
EcoT14I 275 CCAAGG C^C[AT][AT]GG
FauI 306 GCGGG CCCGC(4/6)
HaeII 15 GGCGCT [GA]GCGC^[CT]
HhaI 16 GCGC GCG^C
HinP1I 16 GCGC G^CGC
Ksp632I 118 GAAGAG CTCTTC(1/4)
MaeIII 461 GTGAC ^GT[ACGT]AC
MboII 118 GAAGA GAAGA(8/7)
MseI 110 TTAA T^TAA
MwoI 54 GCTTTATCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 93 GCATGC [GA]CATG^[CT]
PflMI 137 CCAATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 259 GAGCTC GAGCT^C
SfaNI 151 GATGC GCATC(5/9)
SmaI 348 CCCGGG CCC^GGG
SphI 93 GCATGC GCATG^C
StyI 275 CCAAGG C^C[AT][AT]GG
TaqI 332 TCGA T^CGA
TfiI 280 GAATC G^A[AT]TC
Tru9I 110 TTAA T^TAA
Tsp45I 461 GTGAC ^GT[GC]AC
TthHB8I 332 TCGA T^CGA
Van91I 137 CCAATCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 348 CCCGGG C^CCGGG
XmaI 348 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AspHI 259 GAGCTC G[AT]GC[AT]^C
363 GTGCAC
AvaI 348 CCCGGG C^[CT]CG[GA]G
409 CCCGAG
AvaII 3 GGTCC G^G[AT]CC
132 GGTCC
BanI 271 GGTGCC G^G[CT][GA]CC
369 GGTGCC
BanII 259 GAGCTC G[GA]GC[CT]^C
316 GGGCTC
BbvI 90 GCTGC GCAGC(8/12)
340 GCTGC
BcnI 348 CCCGG CC^[GC]GG
349 CCGGG
BfaI 79 CTAG C^TAG
238 CTAG
BpmI 324 CTCCAG CTGGAG(16/14)
83 CTGGAG
BsiHKAI 259 GAGCTC G[AT]GC[AT]^C
363 GTGCAC
BsoBI 348 CCCGGG C^[CT]CG[GA]G
409 CCCGAG
BsrI 195 CCAGT ACTGG(1/-1)
244 CCAGT
BstF5I 171 CATCC GGATG(2/0)
150 GGATG
BstNI 174 CCTGG CC^[AT]GG
183 CCAGG
Cac8I 93 GCATGC GC[ACGT]^[ACGT]GC
154 GCCAGC
Cfr13I 3 GGTCC G^G[ACGT]CC
132 GGTCC
DpnII 123 GATC ^GATC
382 GATC
EcoRII 174 CCTGG ^CC[AT]GG
183 CCAGG
Fnu4HI 90 GCTGC GC^[ACGT]GC
340 GCTGC
FokI 171 CATCC GGATG(9/13)
150 GGATG
HapII 303 CCGG C^CGG
349 CCGG
HpaII 303 CCGG C^CGG
349 CCGG
ItaI 90 GCTGC GC^[ACGT]GC
340 GCTGC
MaeI 79 CTAG C^TAG
238 CTAG
MboI 123 GATC ^GATC
382 GATC
MnlI 323 CCTC CCTC(7/6)
86 GAGG
MspI 303 CCGG C^CGG
349 CCGG
MvaI 174 CCTGG CC^[AT]GG
183 CCAGG
NciI 348 CCCGG CC^[GC]GG
349 CCGGG
NdeII 123 GATC ^GATC
382 GATC
Sau3AI 123 GATC ^GATC
382 GATC
Sau96I 3 GGTCC G^G[ACGT]CC
132 GGTCC
TseI 90 GCTGC G^C[AT]GC
340 GCTGC
XcmI 72 CCAGCTTCTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
137 CCAATCGGTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AluI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BssKI HinfI HphI
NlaIII NlaIV PleI ScrFI TspRI