Restriction map for
pFN21AE6346
##################################
# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AclI AcsI AflIII AhdI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AvaI AviII BamHI BbrPI
BbvCI BcgI BciVI BclI BglI
BglII BsaAI BsaBI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DraI DrdI DsaI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KspI
MaeII MamI MfeI MflI MluI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 150 GGTACC G^GTACC
AccI 1225 GTCTAC GT^[AC][GT]AC
AccIII 81 TCCGGA T^CCGGA
AflII 610 CTTAAG C^TTAAG
AlwI 660 GATCC GGATC(4/5)
Aor51HI 440 AGCGCT AGC^GCT
ApaI 965 GGGCCC GGGCC^C
Asp718I 150 GGTACC G^GTACC
AvrII 48 CCTAGG C^CTAGG
BbeI 282 GGCGCC GGCGC^C
BbsI 1096 GTCTTC GAAGAC(2/6)
BfrI 610 CTTAAG C^TTAAG
BlnI 48 CCTAGG C^CTAGG
BlpI 582 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 582 GCTTAGC GC^T[ACGT]AGC
BpuAI 1096 GTCTTC GAAGAC(2/6)
BseAI 81 TCCGGA T^CCGGA
BsmI 123 GAATGC GAATGC(1/-1)
BspEI 81 TCCGGA T^CCGGA
BspHI 1109 TCATGA T^CATGA
BsrDI 248 GCAATG GCAATG(2/0)
BstAPI 587 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 140 CCAGCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 582 GCTTAGC GC^T[ACGT]AGC
DpnII 660 GATC ^GATC
DraIII 431 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 440 AGCGCT AGC^GCT
HgaI 4 GACGC GACGC(5/10)
KasI 282 GGCGCC G^GCGCC
KpnI 150 GGTACC GGTAC^C
MaeIII 1168 GTCAC ^GT[ACGT]AC
MboI 660 GATC ^GATC
MroI 81 TCCGGA T^CCGGA
MseI 611 TTAA T^TAA
NarI 282 GGCGCC GG^CGCC
NdeII 660 GATC ^GATC
PstI 385 CTGCAG CTGCA^G
PvuII 588 CAGCTG CAG^CTG
RcaI 1109 TCATGA T^CATGA
Sau3AI 660 GATC ^GATC
ScaI 173 AGTACT AGT^ACT
SfaNI 868 GATGC GCATC(5/9)
SfcI 385 CTGCAG C^T[GA][CT]AG
SfoI 282 GGCGCC GGC^GCC
SmlI 610 CTTAAG C^T[CT][GA]AG
Tru9I 611 TTAA T^TAA
Tsp45I 1168 GTCAC ^GT[GC]AC
Tsp509I 919 AATT ^AATT
XcmI 629 CCACCCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 1230 CGCG CG^CG
1232 CGCG
AcyI 4 GACGCC G[GA]^CG[CT]C
282 GGCGCC
AgeI 202 ACCGGT A^CCGGT
551 ACCGGT
AvaII 92 GGACC G^G[AT]CC
772 GGACC
BcnI 433 CCGGG CC^[GC]GG
530 CCCGG
BfaI 49 CTAG C^TAG
955 CTAG
BsaHI 4 GACGCC G[GA]^CG[CT]C
282 GGCGCC
BsaI 199 GAGACC GGTCTC(1/5)
304 GAGACC
BspMI 55 GCAGGT ACCTGC(4/8)
826 GCAGGT
BsrFI 202 ACCGGT [GA]^CCGG[CT]
551 ACCGGT
BstF5I 1134 CATCC GGATG(2/0)
867 GGATG
BstUI 1230 CGCG CG^CG
1232 CGCG
Cfr10I 202 ACCGGT [GA]^CCGG[CT]
551 ACCGGT
EcoNI 969 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1116 CCTCCGCCAGG
FokI 1134 CATCC GGATG(9/13)
867 GGATG
HaeII 282 GGCGCC [GA]GCGC^[CT]
440 AGCGCT
Hin1I 4 GACGCC G[GA]^CG[CT]C
282 GGCGCC
HindIII 233 AAGCTT A^AGCTT
682 AAGCTT
MaeI 49 CTAG C^TAG
955 CTAG
MspA1I 588 CAGCTG C[AC]G^C[GT]G
768 CAGCGG
MvnI 1230 CGCG CG^CG
1232 CGCG
NciI 433 CCGGG CC^[GC]GG
530 CCCGG
NspI 752 GCATGT [GA]CATG^[CT]
1045 GCATGT
PflMI 8 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
633 CCAGGTGCTGG
PinAI 202 ACCGGT A^CCGGT
551 ACCGGT
SapI 336 GCTCTTC GCTCTTC(1/4)
1056 GCTCTTC
Van91I 8 CCAGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
633 CCAGGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwNI BalI
BanI BanII BbvI BmyI BpmI
BsaJI BsaWI BsgI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I DdeI DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MboII MluNI MnlI MscI
MslI MspI MvaI MwoI NlaIII
NlaIV PleI RsaI Sau96I ScrFI
StuI StyI TseI TspRI