(Circular) (Six-base) MAPSORT of: pFN21AE6346.seq Check: 5285 from: 1 to: 6001 pFN21AE6346 6001 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2051 2051 Size: 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3231 3344 1937 Size: 1294 113 4594 Fragments arranged by size: 4594 1294 113 Acc65I G'GTAC_C Cuts at: 2155 3322 2155 Size: 1167 4834 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2167 4064 5448 5716 1632 Size: 535 1897 1384 268 1917 Fragments arranged by size: 1917 1897 1384 535 268 AfeI AGC'GCT Cuts at: 2447 2447 Size: 6001 AflII C'TTAA_G Cuts at: 820 1017 2615 820 Size: 197 1598 4206 Fragments arranged by size: 4206 1598 197 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 2207 2556 4832 2207 Size: 349 2276 3376 Fragments arranged by size: 3376 2276 349 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 2639 3179 1166 Size: 1473 540 3988 Fragments arranged by size: 3988 1473 540 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2780 1184 Size: 1596 4405 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2447 2479 2447 Size: 32 5969 AlwNI CAG_nnn'CTG Cuts at: 2332 2467 2644 3744 5316 2332 Size: 135 177 1100 1572 3017 Fragments arranged by size: 3017 1572 1100 177 135 ApaI G_GGCC'C Cuts at: 2974 2974 Size: 6001 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 AvrII C'CTAG_G Cuts at: 2053 2053 Size: 6001 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2537 2570 2537 Size: 33 5968 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2155 2287 2545 2598 3322 Size: 325 217 627 368 132 258 53 724 Cuts at: 3322 4777 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 724 627 368 325 258 217 132 53 BanII G_rGCy'C Cuts at: 1391 2262 2361 2787 2954 2974 3320 1391 Size: 871 99 426 167 20 346 4072 Fragments arranged by size: 4072 871 426 346 167 99 20 BbeI G_GCGC'C Cuts at: 2291 2291 Size: 6001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3094 928 Size: 971 1195 3835 Fragments arranged by size: 3835 1195 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4612 136 Size: 107 122 71 631 605 2940 1525 Fragments arranged by size: 2940 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 2588 3418 2588 Size: 830 5171 Bme1580I G_kGCm'C Cuts at: 1790 2548 2974 4062 5218 1790 Size: 758 426 1088 1156 2573 Fragments arranged by size: 2573 1156 1088 758 426 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2725 3732 4690 Size: 416 53 83 399 24 1308 1007 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1308 1007 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2222 2345 2999 4661 1239 Size: 983 123 654 1662 2579 Fragments arranged by size: 2579 1662 983 654 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2198 2303 4664 882 Size: 617 699 105 2361 2219 Fragments arranged by size: 2361 2219 699 617 105 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2288 3808 4190 Size: 53 83 186 936 477 278 1520 382 Cuts at: 4190 4825 275 Size: 635 1451 Fragments arranged by size: 1520 1451 936 635 477 382 278 186 83 53 BsaWI w'CCGG_w Cuts at: 2086 2207 2556 4434 4832 5106 5253 5664 2086 Size: 121 349 1878 398 274 147 411 2423 Fragments arranged by size: 2423 1878 411 398 349 274 147 121 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 1178 Size: 30 165 30 1143 30 4603 Fragments arranged by size: 4603 1143 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6001 BseYI C'CCAG_C Cuts at: 2100 2797 3190 5204 5700 2100 Size: 697 393 2014 496 2401 Fragments arranged by size: 2401 2014 697 496 393 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2195 2924 3311 1163 Size: 573 459 729 387 3853 Fragments arranged by size: 3853 729 573 459 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4062 4147 5218 4062 Size: 85 1071 4845 Fragments arranged by size: 4845 1071 85 BsmI GAATG_Cn' Cuts at: 2134 3512 3605 2134 Size: 1378 93 4530 Fragments arranged by size: 4530 1378 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2361 2548 2787 2954 2974 3320 Size: 399 472 99 187 239 167 20 346 Cuts at: 3320 4062 4147 5218 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 346 239 187 167 99 85 20 BspEI T'CCGG_A Cuts at: 2086 5664 2086 Size: 3578 2423 BspHI T'CATG_A Cuts at: 3114 3890 5620 3114 Size: 776 1730 3495 Fragments arranged by size: 3495 1730 776 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2051 2822 3356 844 Size: 1207 771 534 3489 Fragments arranged by size: 3489 1207 771 534 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2260 4496 4670 65 Size: 1183 1012 2236 174 1396 Fragments arranged by size: 2236 1396 1183 1012 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 2556 4645 4832 1700 Size: 110 397 349 2089 187 2869 Fragments arranged by size: 2869 2089 397 349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2598 794 Size: 1804 4197 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2152 Size: 6001 BstYI r'GATC_y Cuts at: 1972 3331 3698 4083 4100 5552 5638 5650 5996 Size: 1359 367 385 17 1452 86 12 346 Cuts at: 5996 1972 Size: 1977 Fragments arranged by size: 1977 1452 1359 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4510 Fragments arranged by size: 4510 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2031 2954 3517 4311 4331 2031 Size: 923 563 794 20 3701 Fragments arranged by size: 3701 923 794 563 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DraIII CAC_nnn'GTG Cuts at: 2441 2441 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 3055 3163 Size: 54 1425 50 275 18 816 409 108 Cuts at: 3163 3448 4337 5966 8 Size: 285 889 1629 43 Fragments arranged by size: 1629 1425 889 816 409 285 275 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2348 2945 3068 3931 1353 Size: 995 597 123 863 3423 Fragments arranged by size: 3423 995 863 597 123 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2203 3113 4588 4962 5108 387 Size: 1064 138 614 910 1475 374 146 1280 Fragments arranged by size: 1475 1280 1064 910 614 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2167 2222 2345 2999 4064 4661 5448 Size: 393 535 55 123 654 1065 597 787 Cuts at: 5448 5716 1239 Size: 268 1524 Fragments arranged by size: 1524 1065 787 654 597 535 393 268 123 55 EcoNI CCTnn'n_nnAGG Cuts at: 2978 3125 2978 Size: 147 5854 EcoO109I rG'GnC_Cy Cuts at: 2215 2329 2970 2971 2215 Size: 114 641 1 5245 Fragments arranged by size: 5245 641 114 1 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2291 2449 5148 1678 Size: 613 158 2699 2531 Fragments arranged by size: 2699 2531 613 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4642 4674 4716 4748 1373 Size: 32 135 32 3070 32 42 32 2626 Fragments arranged by size: 3070 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 2238 2687 748 Size: 1490 449 4062 Fragments arranged by size: 4062 1490 449 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2287 2287 Size: 6001 KpnI G_GTAC'C Cuts at: 2159 3326 2159 Size: 1167 4834 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 3057 3165 10 Size: 54 1425 343 816 409 108 2846 Fragments arranged by size: 2846 1425 816 409 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 2639 2678 3113 3179 3959 4318 Size: 648 1152 321 39 435 66 780 359 Cuts at: 4318 4477 518 Size: 159 2042 Fragments arranged by size: 2042 1152 780 648 435 359 321 159 66 39 MspA1I CmG'CkG Cuts at: 1606 2595 2775 3417 4094 5242 5487 1606 Size: 989 180 642 677 1148 245 2120 Fragments arranged by size: 2120 1148 989 677 642 245 180 NarI GG'CG_CC Cuts at: 2288 2288 Size: 6001 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4510 Fragments arranged by size: 4510 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2761 3054 3359 4904 1132 Size: 97 1532 293 305 1545 2229 Fragments arranged by size: 2229 1545 1532 305 293 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2019 2644 1108 Size: 911 625 4465 Fragments arranged by size: 4465 911 625 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2970 2970 Size: 6001 PstI C_TGCA'G Cuts at: 830 2394 3353 830 Size: 1564 959 3478 Fragments arranged by size: 3478 1564 959 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2595 2595 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 2348 3068 2348 Size: 720 5281 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 2180 4249 5961 2180 Size: 2069 1712 2220 Fragments arranged by size: 2220 2069 1712 SfcI C'TryA_G Cuts at: 826 1045 2390 3349 4484 5165 5356 826 Size: 219 1345 959 1135 681 191 1471 Fragments arranged by size: 1471 1345 1135 959 681 219 191 SfoI GGC'GCC Cuts at: 2289 2289 Size: 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 4105 5006 5268 820 Size: 197 926 672 1490 901 262 1553 Fragments arranged by size: 1553 1490 926 901 672 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 2267 2654 2817 3131 1517 Size: 750 387 163 314 4387 Fragments arranged by size: 4387 750 387 314 163 StyI C'CwwG_G Cuts at: 513 1065 2004 2053 2262 2649 3440 513 Size: 552 939 49 209 387 791 3074 Fragments arranged by size: 3074 939 791 552 387 209 49 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2563 2651 4028 4045 4198 4383 4828 Size: 1255 983 88 1377 17 153 185 445 Cuts at: 4828 325 Size: 1498 Fragments arranged by size: 1498 1377 1255 983 445 185 153 88 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2178 4247 5959 Size: 274 80 33 51 1443 201 2069 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 2069 1712 1443 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2676 2708 5961 5993 2676 Size: 32 3253 32 2684 Fragments arranged by size: 3253 2684 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2641 2641 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI