################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AhdI AocI ApaI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HincII HindII HindIII HpaI KspI MfeI MluI MroI MseI MspA1I MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tru9I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 596 GGTACC G^GTACC AccII 96 CGCG CG^CG AcyI 340 GGCGCC G[GA]^CG[CT]C Alw44I 649 GTGCAC G^TGCAC Aor51HI 219 AGCGCT AGC^GCT ApaLI 649 GTGCAC G^TGCAC Asp718I 596 GGTACC G^GTACC AspHI 649 GTGCAC G[AT]GC[AT]^C AvaI 161 CCCGGG C^[CT]CG[GA]G BalI 686 TGGCCA TGG^CCA BanII 358 GAGCCC G[GA]GC[CT]^C BbeI 340 GGCGCC GGCGC^C BbvCI 215 CCTCAGC CCTCAGC(-5/-2) BclI 567 TGATCA T^GATCA BfaI 133 CTAG C^TAG BglI 85 GCCAAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1001 AGATCT A^GATCT BsaAI 879 CACGTA [CT]AC^GT[GA] BsaBI 711 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 340 GGCGCC G[GA]^CG[CT]C BsiHKAI 649 GTGCAC G[AT]GC[AT]^C BsoBI 161 CCCGGG C^[CT]CG[GA]G BsrDI 285 CATTGC GCAATG(2/0) BstAPI 1122 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1135 GGTAACC G^GT[ACGT]ACC BstPI 1135 GGTAACC G^GT[ACGT]ACC BstUI 96 CGCG CG^CG DsaI 632 CCGTGG C^C[GA][CT]GG Eco47III 219 AGCGCT AGC^GCT EcoNI 31 CCTGAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1135 GGTAACC G^GT[ACGT]ACC FbaI 567 TGATCA T^GATCA HgaI 97 GCGTC GACGC(5/10) Hin1I 340 GGCGCC G[GA]^CG[CT]C KasI 340 GGCGCC G^GCGCC KpnI 596 GGTACC GGTAC^C MaeI 133 CTAG C^TAG MamI 711 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 686 TGGCCA TGG^CCA MscI 686 TGGCCA TGG^CCA MvnI 96 CGCG CG^CG NaeI 1081 GCCGGC GCC^GGC NarI 340 GGCGCC GG^CGCC NgoMIV 1081 GCCGGC G^CCGGC PleI 700 GAGTC GAGTC(4/5) SapI 1035 GCTCTTC GCTCTTC(1/4) SexAI 254 ACCTGGT A^CC[AT]GGT SfoI 340 GGCGCC GGC^GCC SmaI 161 CCCGGG CCC^GGG StuI 852 AGGCCT AGG^CCT Tsp509I 464 AATT ^AATT XmaCI 161 CCCGGG C^CCGGG XmaI 161 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AfaI 597 GTAC GT^AC 882 GTAC AgeI 154 ACCGGT A^CCGGT 1014 ACCGGT AlwI 674 GATCC GGATC(4/5) 753 GATCC AlwNI 728 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1123 CAGAACCTG BbsI 814 GAAGAC GAAGAC(2/6) 909 GAAGAC BbvI 110 GCAGC GCAGC(8/12) 405 GCTGC BpuAI 814 GAAGAC GAAGAC(2/6) 909 GAAGAC BsaI 151 GAGACC GGTCTC(1/5) 1153 GAGACC BsaWI 154 ACCGGT [AT]^CCGG[AT] 1014 ACCGGT BsgI 319 GTGCAG GTGCAG(16/14) 1018 GTGCAG BsmAI 151 GAGAC GTCTC(1/5) 1153 GAGAC BsmFI 419 GGGAC GGGAC(10/14) 747 GTCCC BstF5I 1050 CATCC GGATG(2/0) 636 GGATG BstYI 752 AGATCC [GA]^GATC[CT] 1001 AGATCT DdeI 32 CTGAG C^T[ACGT]AG 216 CTCAG DraII 143 GGGGCCT [GA]G^G[ACGT]CC[CT] 482 AGGCCCC EarI 1036 CTCTTC CTCTTC(1/4) 697 GAAGAG EcoO109I 143 GGGGCCT [GA]G^G[ACGT]CC[CT] 482 AGGCCCC FokI 1050 CATCC GGATG(9/13) 636 GGATG Ksp632I 1036 CTCTTC CTCTTC(1/4) 697 GAAGAG MaeII 412 ACGT A^CGT 880 ACGT MflI 752 AGATCC [GA]^GATC[CT] 1001 AGATCT PflMI 546 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 727 CCAGCGCCTGG PinAI 154 ACCGGT A^CCGGT 1014 ACCGGT RsaI 597 GTAC GT^AC 882 GTAC SfaNI 711 GATGC GCATC(5/9) 943 GATGC SmlI 805 CTCAAG C^T[CT][GA]AG 840 CTTGAG TfiI 44 GATTC G^A[AT]TC 831 GATTC TseI 110 GCAGC G^C[AT]GC 405 GCTGC TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^ 1097 CACTG Van91I 546 CCACATTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 727 CCAGCGCCTGG XhoII 752 AGATCC [GA]^GATC[CT] 1001 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AluI AvaII BanI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BspMI BsrFI BssKI BstNI BstXI Cac8I CfoI Cfr10I Cfr13I DpnII DraIII EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TaqI Tsp45I TthHB8I XcmI