Restriction map for
pFN21AE6308
(Circular) (Six-base) MAPSORT of: pFN21AE6308.seq Check: 1972 from: 1 to: 5884
pFN21AE6308 5884 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2641 2641
Size: 5884
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3694 278
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3227 1937
Size: 1290 4594
Acc65I G'GTAC_C
Cuts at: 2601 3205 2601
Size: 604 5280
AclI AA'CG_TT
Cuts at: 1563 4011 4384 1563
Size: 2448 373 3063
Fragments arranged by size:
3063 2448 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3947 5331 5599 1632
Size: 1301 1014 1384 268 1917
Fragments arranged by size:
1917 1384 1301 1014 268
AfeI AGC'GCT
Cuts at: 2226 2226
Size: 5884
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5687
AflIII A'CryG_T
Cuts at: 1203 4783 5760 1203
Size: 3580 977 1327
Fragments arranged by size:
3580 1327 977
AgeI A'CCGG_T
Cuts at: 2159 3019 4715 2159
Size: 860 1696 3328
Fragments arranged by size:
3328 1696 860
AhdI GACnn_n'nnGTC
Cuts at: 4613 4613
Size: 5884
AleI CACnn'nnGTG
Cuts at: 1166 2441 2651 1166
Size: 1275 210 4399
Fragments arranged by size:
4399 1275 210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5884
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2279 2311 2279
Size: 32 5852
AlwNI CAG_nnn'CTG
Cuts at: 2738 3133 3627 5199 2738
Size: 395 494 1572 3423
Fragments arranged by size:
3423 1572 494 395
ApaLI G'TGCA_C
Cuts at: 2654 3941 5097 2654
Size: 1287 1156 3441
Fragments arranged by size:
3441 1287 1156
ApoI r'AATT_y
Cuts at: 1493 3193 3419 3571 5680 1493
Size: 1700 226 152 2109 1697
Fragments arranged by size:
2109 1700 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4438 160
Size: 872 3406 1606
Fragments arranged by size:
3406 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 3209 1870
Size: 73 223 1043 4545
Fragments arranged by size:
4545 1043 223 73
BamHI G'GATC_C
Cuts at: 3214 3581 3214
Size: 367 5517
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 2601 3172 3205 4660
Size: 325 217 627 558 256 571 33 1455
Cuts at: 4660 618
Size: 1842
Fragments arranged by size:
1842 1455 627 571 558 325 256 217
33
BanII G_rGCy'C
Cuts at: 1391 2367 3203 1391
Size: 976 836 4072
Fragments arranged by size:
4072 976 836
BbeI G_GCGC'C
Cuts at: 2349 2349
Size: 5884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2826 2921 928
Size: 971 927 95 3891
Fragments arranged by size:
3891 971 927 95
BbvCI CC'TCA_GC
Cuts at: 2221 2221
Size: 5884
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4075 4109 1903
Size: 34 2138 34 3678
Fragments arranged by size:
3678 2138 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3776 4986 3776
Size: 1210 4674
BclI T'GATC_A
Cuts at: 1548 2572 1548
Size: 1024 4860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2096 4495 136
Size: 107 122 71 631 605 424 2399 1525
Fragments arranged by size:
2399 1525 631 605 424 122 107 71
BglII A'GATC_T
Cuts at: 3006 5879 3006
Size: 2873 3011
BlpI GC'TnA_GC
Cuts at: 3301 3301
Size: 5884
Bme1580I G_kGCm'C
Cuts at: 1790 2658 3177 3945 5101 1790
Size: 868 519 768 1156 2573
Fragments arranged by size:
2573 1156 868 768 519
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5884
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3615 4573 442
Size: 416 53 83 399 24 2198 958 1753
Fragments arranged by size:
2198 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5884
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2174 2558 2882 2975 4544 1239
Size: 935 384 324 93 1569 2579
Fragments arranged by size:
2579 1569 935 384 324 93
Bpu10I CC'TnA_GC
Cuts at: 2221 2221
Size: 5884
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2795 2866 4009 4874 5172 2795
Size: 71 1143 865 298 3507
Fragments arranged by size:
3507 1143 865 298 71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2150 3152 4547 882
Size: 617 651 1002 1395 2219
Fragments arranged by size:
2219 1395 1002 651 617
BsaAI yAC'GTr
Cuts at: 493 1204 2886 3675 493
Size: 711 1682 789 2702
Fragments arranged by size:
2702 1682 789 711
BsaBI GATnn'nnATC
Cuts at: 2720 5878 2720
Size: 3158 2726
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2346 3691 4073 4708
Size: 53 83 186 936 813 1345 382 635
Cuts at: 4708 275
Size: 1451
Fragments arranged by size:
1451 1345 936 813 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2159 3019 4317 4715 4989 5136 5547 2159
Size: 860 1298 398 274 147 411 2496
Fragments arranged by size:
2496 1298 860 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2570 2600 1178
Size: 30 165 30 1167 30 4462
Fragments arranged by size:
4462 1167 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2209 2972 3134 3137 1186
Size: 1023 763 162 3 3933
Fragments arranged by size:
3933 1023 763 162 3
BseYI C'CCAG_C
Cuts at: 2491 2789 5087 5583 2491
Size: 298 2298 496 2792
Fragments arranged by size:
2792 2298 496 298
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 3044 1163
Size: 573 609 699 4003
Fragments arranged by size:
4003 699 609 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3334 4095 5123 1540
Size: 275 186 1333 761 1028 2301
Fragments arranged by size:
2301 1333 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2658 3945 4030 5101 2658
Size: 1287 85 1071 3441
Fragments arranged by size:
3441 1287 1071 85
BsmI GAATG_Cn'
Cuts at: 3395 3488 3395
Size: 93 5791
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2658 3177 3203 3945 4030 5101
Size: 399 577 291 519 26 742 85 1071
Cuts at: 5101 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 577 519 399 291 85
26
BspEI T'CCGG_A
Cuts at: 5547 5547
Size: 5884
BspHI T'CATG_A
Cuts at: 3773 5503 3773
Size: 1730 4154
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2622 2641 3141 3239 844
Size: 1778 19 500 98 3489
Fragments arranged by size:
3489 1778 500 98 19
BsrBI CCG'CTC
Cuts at: 1394 1823 3771 1394
Size: 429 1948 3507
Fragments arranged by size:
3507 1948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2289 4379 4553 65
Size: 1183 1041 2090 174 1396
Fragments arranged by size:
2090 1396 1183 1041 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2159 3019 3030 3086 4528 4715 1700
Size: 110 349 860 11 56 1442 187 2869
Fragments arranged by size:
2869 1442 860 349 187 110 56 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5884
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5884
BssSI C'ACGA_G
Cuts at: 3944 4956 3944
Size: 1012 4872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3133 794
Size: 2339 3545
BstBI TT'CG_AA
Cuts at: 4688 4688
Size: 5884
BstEII G'GTnAC_C
Cuts at: 1184 3140 1184
Size: 1956 3928
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 3080 3169 2689
Size: 391 89 5404
Fragments arranged by size:
5404 391 89
BstYI r'GATC_y
Cuts at: 1972 2757 3006 3214 3581 3966 3983 5435 5521
Size: 785 249 208 367 385 17 1452 86
Cuts at: 5521 5533 5879 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 785 385 367 346 249 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2637 513
Size: 552 1572 3760
Fragments arranged by size:
3760 1572 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2788 487
Size: 683 56 1562 3583
Fragments arranged by size:
3583 1562 683 56
BtsI GCAGTG_nn'
Cuts at: 3400 4194 4214 3400
Size: 794 20 5070
Fragments arranged by size:
5070 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5884
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5849
DraI TTT'AAA
Cuts at: 3188 3188
Size: 5884
DraIII CAC_nnn'GTG
Cuts at: 2068 2263 2557 2068
Size: 195 294 5395
Fragments arranged by size:
5395 294 195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4891 809
Size: 507 3575 1802
Fragments arranged by size:
3575 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2464 2691 2919
Size: 54 1425 50 275 18 634 227 228
Cuts at: 2919 3331 4220 5849 8
Size: 412 889 1629 43
Fragments arranged by size:
1629 1425 889 634 412 275 228 227
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3331 1537
Size: 275 1519 4090
Fragments arranged by size:
4090 1519 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2697 3047 3814 1353
Size: 1344 350 767 3423
Fragments arranged by size:
3423 1344 767 350
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2578 4471 4845 4991 387
Size: 1064 138 989 1893 374 146 1280
Fragments arranged by size:
1893 1280 1064 989 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2174 2558 2882 2933 2975 3947 4544
Size: 393 542 384 324 51 42 972 597
Cuts at: 4544 5331 5599 1239
Size: 787 268 1524
Fragments arranged by size:
1524 972 787 597 542 393 384 324
268 51 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2040 2040
Size: 5884
EcoO109I rG'GnC_Cy
Cuts at: 2149 2488 2149
Size: 339 5545
EcoRI G'AATT_C
Cuts at: 3193 3571 3193
Size: 378 5506
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 5884
FspI TGC'GCA
Cuts at: 4390 4390
Size: 5884
HaeII r_GCGC'y
Cuts at: 1678 2228 2349 2738 2790 3064 5031 1678
Size: 550 121 389 52 274 1967 2531
Fragments arranged by size:
2531 1967 550 389 274 121 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2239 2271 2570 2602 2855
Size: 32 135 32 667 32 299 32 253
Cuts at: 2855 2887 4525 4557 4599 4631 1373
Size: 32 1638 32 42 32 2626
Fragments arranged by size:
2626 1638 667 299 253 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3228 3474 669
Size: 988 281 1290 246 3079
Fragments arranged by size:
3079 1290 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5884
HpaI GTT'AAC
Cuts at: 3474 3474
Size: 5884
KasI G'GCGC_C
Cuts at: 2345 2345
Size: 5884
KpnI G_GTAC'C
Cuts at: 2605 3209 2605
Size: 604 5280
MfeI C'AATT_G
Cuts at: 3483 3483
Size: 5884
MluI A'CGCG_T
Cuts at: 5760 5760
Size: 5884
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4998 5182 1305
Size: 3693 184 2007
Fragments arranged by size:
3693 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2693 10
Size: 54 1425 343 861 3201
Fragments arranged by size:
3201 1425 861 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2651 2687 2714 3152 3842 4201
Size: 648 1275 210 36 27 438 690 359
Cuts at: 4201 4360 518
Size: 159 2042
Fragments arranged by size:
2042 1275 690 648 438 359 210 159
36 27
MspA1I CmG'CkG
Cuts at: 1606 3300 3977 5125 5370 1606
Size: 1694 677 1148 245 2120
Fragments arranged by size:
2120 1694 1148 677 245
NaeI GCC'GGC
Cuts at: 3088 3088
Size: 5884
NarI GG'CG_CC
Cuts at: 2346 2346
Size: 5884
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5332
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5884
NgoMIV G'CCGG_C
Cuts at: 3086 3086
Size: 5884
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5884
NotI GC'GGCC_GC
Cuts at: 3331 3331
Size: 5884
NspI r_CATG'y
Cuts at: 1132 1229 3242 4787 1132
Size: 97 2013 1545 2229
Fragments arranged by size:
2229 2013 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2367 1348
Size: 1019 4865
PciI A'CATG_T
Cuts at: 4783 4783
Size: 5884
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2557 2738 1108
Size: 1449 181 4254
Fragments arranged by size:
4254 1449 181
PfoI T'CCnGG_A
Cuts at: 2760 3045 2760
Size: 285 5599
PmeI GTTT'AAAC
Cuts at: 3188 3188
Size: 5884
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3953 3985 5466 5498 3953
Size: 32 1481 32 4339
Fragments arranged by size:
4339 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5884
PsiI TTA'TAA
Cuts at: 3454 3454
Size: 5884
PstI C_TGCA'G
Cuts at: 830 2117 2415 2619 3006 3236 830
Size: 1287 298 204 387 230 3478
Fragments arranged by size:
3478 1287 387 298 230 204
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5884
SalI G'TCGA_C
Cuts at: 1936 3226 1936
Size: 1290 4594
SapI GCTCTTCn'nnn_
Cuts at: 3047 3047
Size: 5884
SbfI CC_TGCA'GG
Cuts at: 3236 3236
Size: 5884
ScaI AGT'ACT
Cuts at: 4132 5844 4132
Size: 1712 4172
SexAI A'CCwGG_T
Cuts at: 2259 2259
Size: 5884
SfcI C'TryA_G
Cuts at: 826 1045 2113 2143 2411 2615 3002 3232 4367
Size: 219 1068 30 268 204 387 230 1135
Cuts at: 4367 5048 5239 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1068 681 387 268 230 219
204 191 30
SfoI GGC'GCC
Cuts at: 2347 2347
Size: 5884
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5884
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5884
SmaI CCC'GGG
Cuts at: 1872 2168 3211 1872
Size: 296 1043 4545
Fragments arranged by size:
4545 1043 296
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 2845 3988 4889 5151 820
Size: 197 926 867 35 1143 901 262 1553
Fragments arranged by size:
1553 1143 926 901 867 262 197 35
SnaBI TAC'GTA
Cuts at: 493 3675 493
Size: 3182 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5884
SphI G_CATG'C
Cuts at: 1132 3242 1132
Size: 2110 3774
SspI AAT'ATT
Cuts at: 5 52 3808 5
Size: 47 3756 2081
Fragments arranged by size:
3756 2081 47
StuI AGG'CCT
Cuts at: 1517 2859 1517
Size: 1342 4542
StyI C'CwwG_G
Cuts at: 513 1065 3323 513
Size: 552 2258 3074
Fragments arranged by size:
3074 2258 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 3911 3928 4081 4266 4711 325
Size: 1255 1251 1080 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1251 1080 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4130 5842 96
Size: 274 80 33 51 1443 2153 1712 138
Fragments arranged by size:
2153 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5844 5876 5844
Size: 32 5852
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5884
XbaI T'CTAG_A
Cuts at: 3220 3220
Size: 5884
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2437 2474 2533 3043 3077 2437
Size: 37 59 510 34 5244
Fragments arranged by size:
5244 510 59 37 34
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5884
XmaI C'CCGG_G
Cuts at: 1870 2166 3209 1870
Size: 296 1043 4545
Fragments arranged by size:
4545 1043 296
XmnI GAAnn'nnTTC
Cuts at: 4013 4013
Size: 5884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3692 276
Size: 53 83 186 936 2158 2468
Fragments arranged by size:
2468 2158 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SfiI SrfI SwaI