Restriction map for
pFN21AE2572
##################################
# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
AluI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstYI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DraII DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tth111I TthHB8I XbaI XhoI XhoII
XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AcsI 208 AAATTC [GA]^AATT[CT]
AfaI 339 GTAC GT^AC
AlwI 85 GGATC GGATC(4/5)
AlwNI 337 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 208 AAATTC [GA]^AATT[CT]
AspHI 60 GAGCAC G[AT]GC[AT]^C
BalI 257 TGGCCA TGG^CCA
BanII 349 GAGCCC G[GA]GC[CT]^C
BbsI 196 GAAGAC GAAGAC(2/6)
BlpI 30 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 30 GCTAAGC GC^T[ACGT]AGC
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaI 289 GAGACC GGTCTC(1/5)
BsaWI 170 ACCGGA [AT]^CCGG[AT]
BseRI 346 GAGGAG GAGGAG(10/8)
BsgI 334 GTGCAG GTGCAG(16/14)
BsiEI 168 CGACCG CG[GA][CT]^CG
BsiHKAI 60 GAGCAC G[AT]GC[AT]^C
BsmAI 289 GAGAC GTCTC(1/5)
BsmFI 113 GGGAC GGGAC(10/14)
BsmI 305 GAATGC GAATGC(1/-1)
BstEII 129 GGTCACC G^GT[ACGT]ACC
BstF5I 213 CATCC GGATG(2/0)
BstPI 129 GGTCACC G^GT[ACGT]ACC
Cac8I 93 GCTGGC GC[ACGT]^[ACGT]GC
CelII 30 GCTAAGC GC^T[ACGT]AGC
CfoI 6 GCGC GCG^C
DdeI 31 CTAAG C^T[ACGT]AG
DpnII 86 GATC ^GATC
DrdI 121 GACGCCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 293 CCGTGG C^C[GA][CT]GG
EaeI 257 TGGCCA [CT]^GGCC[GA]
EcoO65I 129 GGTCACC G^GT[ACGT]ACC
FokI 213 CATCC GGATG(9/13)
HhaI 6 GCGC GCG^C
HinP1I 6 GCGC G^CGC
MboI 86 GATC ^GATC
MboII 196 GAAGA GAAGA(8/7)
MluNI 257 TGGCCA TGG^CCA
MscI 257 TGGCCA TGG^CCA
MseI 191 TTAA T^TAA
MspA1I 134 CCGCTG C[AC]G^C[GT]G
NdeII 86 GATC ^GATC
PflMI 324 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 339 GTAC GT^AC
Sau3AI 86 GATC ^GATC
SfaNI 370 GCATC GCATC(5/9)
SmaI 125 CCCGGG CCC^GGG
SmlI 310 CTCAAG C^T[CT][GA]AG
Tru9I 191 TTAA T^TAA
Tsp45I 130 GTCAC ^GT[GC]AC
Tsp509I 209 AATT ^AATT
Van91I 324 CCAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 125 CCCGGG C^CCGGG
XmaI 125 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcyI 52 GGCGTC G[GA]^CG[CT]C
121 GACGCC
AvaI 125 CCCGGG C^[CT]CG[GA]G
251 CCCGAG
BcnI 125 CCCGG CC^[GC]GG
126 CCGGG
BmyI 60 GAGCAC G[AGT]GC[ACT]^C
349 GAGCCC
BpmI 47 CTGGAG CTGGAG(16/14)
343 CTGGAG
BsaHI 52 GGCGTC G[GA]^CG[CT]C
121 GACGCC
BsoBI 125 CCCGGG C^[CT]CG[GA]G
251 CCCGAG
Bsp1286I 60 GAGCAC G[AGT]GC[ACT]^C
349 GAGCCC
BsrI 46 ACTGG ACTGG(1/-1)
146 CCAGT
HapII 126 CCGG C^CGG
171 CCGG
HgaI 121 GACGC GACGC(5/10)
53 GCGTC
Hin1I 52 GGCGTC G[GA]^CG[CT]C
121 GACGCC
HpaII 126 CCGG C^CGG
171 CCGG
MspI 126 CCGG C^CGG
171 CCGG
NciI 125 CCCGG CC^[GC]GG
126 CCGGG
NlaIII 219 CATG CATG^
228 CATG
NlaIV 179 GGAACC GG[ACGT]^[ACGT]CC
348 GGAGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstNI Cfr13I EcoRII EcoT14I
Fnu4HI HaeIII HphI ItaI MaeIII
MnlI MslI MvaI MwoI Sau96I
ScrFI StyI TseI TspRI XcmI