(Linear) (Six-base) MAPSORT of: KIEE2572.seq  Check: 7404  from: 1  to: 375



KIEE2572.orf

 With 182 enzymes: SgfI * 

                        April 14, 2015 09:47  ..

AleI CACnn'nnGTG

Cuts at:      0     253     361     375 
   Size:        253     108      14

  Fragments arranged by size: 

                 253     108      14
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      85     375 
   Size:         85     290
 
AlwNI CAG_nnn'CTG

Cuts at:      0     342     375 
   Size:        342      33
 
ApoI r'AATT_y

Cuts at:      0     208     375 
   Size:        208     167
 
AvaI C'yCGr_G

Cuts at:      0     125     251     375 
   Size:        125     126     124

  Fragments arranged by size: 

                 126     125     124
 
BanII G_rGCy'C

Cuts at:      0     353     375 
   Size:        353      22
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     203     375 
   Size:        203     172
 
BlpI GC'TnA_GC

Cuts at:      0      31     375 
   Size:         31     344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     364     375 
   Size:         68     296      11

  Fragments arranged by size: 

                 296      68      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     375 
   Size:        295      80
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     283     375 
   Size:        283      92
 
BsaHI Gr'CG_yC

Cuts at:      0      53     122     375 
   Size:         53      69     253

  Fragments arranged by size: 

                 253      69      53
 
BsaWI w'CCGG_w

Cuts at:      0     170     375 
   Size:        170     205
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     361     375 
   Size:        361      14
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     375 
   Size:        355      20
 
BsiEI CG_ry'CG

Cuts at:      0     171     375 
   Size:        171     204
 
BsiHKAI G_wGCw'C

Cuts at:      0      64     375 
   Size:         64     311
 
BsmI GAATG_Cn'

Cuts at:      0     311     375 
   Size:        311      64
 
Bsp1286I G_dGCh'C

Cuts at:      0      64     353     375 
   Size:         64     289      22

  Fragments arranged by size: 

                 289      64      22
 
BstEII G'GTnAC_C

Cuts at:      0     129     375 
   Size:        129     246
 
BtgI C'CryG_G

Cuts at:      0     293     375 
   Size:        293      82
 
BtsI GCAGTG_nn'

Cuts at:      0      88     375 
   Size:         88     287
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     127     375 
   Size:        127     248
 
EaeI y'GGCC_r

Cuts at:      0     257     375 
   Size:        257     118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     364     375 
   Size:         68     296      11

  Fragments arranged by size: 

                 296      68      11
 
MscI TGG'CCA

Cuts at:      0     259     375 
   Size:        259     116
 
MslI CAynn'nnrTG

Cuts at:      0     217     253     361     375 
   Size:        217      36     108      14

  Fragments arranged by size: 

                 217     108      36      14
 
MspA1I CmG'CkG

Cuts at:      0     136     375 
   Size:        136     239
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     330     375 
   Size:        330      45
 
SmaI CCC'GGG

Cuts at:      0     127     375 
   Size:        127     248
 
SmlI C'TyrA_G

Cuts at:      0     310     375 
   Size:        310      65
 
StyI C'CwwG_G

Cuts at:      0      11     284     317     375 
   Size:         11     273      33      58

  Fragments arranged by size: 

                 273      58      33      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     373     375 
   Size:        373       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      43     252     291     375 
   Size:         43     209      39      84

  Fragments arranged by size: 

                 209      84      43      39
 
XmaI C'CCGG_G

Cuts at:      0     125     375 
   Size:        125     250
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI     ApoI     AvaI    BanII     BbsI     BlpI
     BpmI    BpuEI     BsaI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI     BsmI Bsp1286I   BstEII     BtgI     BtsI     DrdI     EaeI
  Eco57MI     MscI     MslI   MspA1I    PflMI     SmaI     SmlI     StyI
    TaqII     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaXI    BseYI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmnI
     ZraI