################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I TspRI Tth111I XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AlwI 444 GGATC GGATC(4/5) AlwNI 401 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 118 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 473 GTGCTC G[AT]GC[AT]^C BcnI 521 CCCGG CC^[GC]GG BmyI 473 GTGCTC G[AGT]GC[ACT]^C BsiHKAI 473 GTGCTC G[AT]GC[AT]^C BsmAI 213 GTCTC GTCTC(1/5) BsmFI 66 GGGAC GGGAC(10/14) Bsp1286I 473 GTGCTC G[AGT]GC[ACT]^C BspHI 333 TCATGA T^CATGA BsrDI 149 GCAATG GCAATG(2/0) BsrI 69 ACTGG ACTGG(1/-1) BstNI 227 CCTGG CC^[AT]GG DdeI 430 CTTAG C^T[ACGT]AG DsaI 451 CCATGG C^C[GA][CT]GG EarI 31 CTCTTC CTCTTC(1/4) EcoRII 227 CCTGG ^CC[AT]GG HapII 522 CCGG C^CGG HinfI 211 GAGTC G^A[ACGT]TC HpaII 522 CCGG C^CGG HphI 425 TCACC GGTGA(8/7) Ksp632I 31 CTCTTC CTCTTC(1/4) MaeIII 38 GTAAC ^GT[ACGT]AC MspA1I 404 CAGCTG C[AC]G^C[GT]G MspI 522 CCGG C^CGG MvaI 227 CCTGG CC^[AT]GG NciI 521 CCCGG CC^[GC]GG NcoI 451 CCATGG C^CATGG NlaIV 26 GGAGCC GG[ACGT]^[ACGT]CC PflMI 17 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 211 GAGTC GAGTC(4/5) PstI 353 CTGCAG CTGCA^G PvuII 404 CAGCTG CAG^CTG RcaI 333 TCATGA T^CATGA SfcI 353 CTGCAG C^T[GA][CT]AG TaqI 415 TCGA T^CGA TthHB8I 415 TCGA T^CGA Van91I 17 CCAAACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 504 TCTAGA T^CTAGA XmnI 118 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 297 AGCT AG^CT 405 AGCT BbvI 403 GCAGC GCAGC(8/12) 352 GCTGC BfaI 295 CTAG C^TAG 505 CTAG BsaJI 451 CCATGG C^C[ACGT][ACGT]GG 498 CCAAGG BsiYI 17 CCAAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 136 CCAACCATAGG BslI 17 CCAAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 136 CCAACCATAGG BssKI 227 CCTGG ^CC[ACGT]GG 521 CCCGG EcoT14I 451 CCATGG C^C[AT][AT]GG 498 CCAAGG Fnu4HI 352 GCTGC GC^[ACGT]GC 403 GCAGC ItaI 352 GCTGC GC^[ACGT]GC 403 GCAGC MaeI 295 CTAG C^TAG 505 CTAG MseI 109 TTAA T^TAA 440 TTAA NlaIII 334 CATG CATG^ 452 CATG ScrFI 227 CCTGG CC^[ACGT]GG 521 CCCGG StyI 451 CCATGG C^C[AT][AT]GG 498 CCAAGG Tru9I 109 TTAA T^TAA 440 TTAA TseI 352 GCTGC G^C[AT]GC 403 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BstF5I DpnII FokI MboI MboII MnlI NdeII Sau3AI Tsp509I