################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BseRI BsiWI BsmBI Bsp1407I BspDI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccI 853 GTATAC GT^[AC][GT]AC AccII 21 CGCG CG^CG AccIII 633 TCCGGA T^CCGGA AcsI 731 AAATTT [GA]^AATT[CT] AflIII 573 ACATGT A^C[GA][CT]GT AlwNI 745 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 731 AAATTT [GA]^AATT[CT] AspI 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 276 TGGCCA TGG^CCA BanII 528 GGGCTC G[GA]GC[CT]^C BclI 809 TGATCA T^GATCA BglII 554 AGATCT A^GATCT BsaWI 633 TCCGGA [AT]^CCGG[AT] BseAI 633 TCCGGA T^CCGGA BsiEI 26 CGGCCG CG[GA][CT]^CG BspEI 633 TCCGGA T^CCGGA BspLU11I 573 ACATGT A^CATGT Bst1107I 853 GTATAC GTA^TAC BstUI 21 CGCG CG^CG BstXI 1034 CCACCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 554 AGATCT [GA]^GATC[CT] BstZ17I 853 GTATAC GTA^TAC CfoI 9 GCGC GCG^C EagI 26 CGGCCG C^GGCCG EclXI 26 CGGCCG C^GGCCG Eco52I 26 CGGCCG C^GGCCG EcoT14I 228 CCAAGG C^C[AT][AT]GG FauI 381 CCCGC CCCGC(4/6) FbaI 809 TGATCA T^GATCA HhaI 9 GCGC GCG^C HinP1I 9 GCGC G^CGC MflI 554 AGATCT [GA]^GATC[CT] MluNI 276 TGGCCA TGG^CCA MroI 633 TCCGGA T^CCGGA MscI 276 TGGCCA TGG^CCA MseI 49 TTAA T^TAA MvnI 21 CGCG CG^CG PaeR7I 220 CTCGAG C^TCGAG PflFI 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 667 GGGTCCC [GA]G^G[AT]CC[CT] PstI 160 CTGCAG CTGCA^G SapI 622 GAAGAGC GCTCTTC(1/4) SmaI 606 CCCGGG CCC^GGG SmlI 220 CTCGAG C^T[CT][GA]AG SspI 650 AATATT AAT^ATT StuI 495 AGGCCT AGG^CCT StyI 228 CCAAGG C^C[AT][AT]GG TfiI 611 GATTC G^A[AT]TC Tru9I 49 TTAA T^TAA Tsp509I 732 AATT ^AATT Tth111I 896 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 220 CTCGAG C^TCGAG XhoII 554 AGATCT [GA]^GATC[CT] XmaCI 606 CCCGGG C^CCGGG XmaI 606 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 202 GACGCC G[GA]^CG[CT]C 466 GACGCC AvaI 220 CTCGAG C^[CT]CG[GA]G 606 CCCGGG BpmI 193 CTCCAG CTGGAG(16/14) 325 CTCCAG BsaHI 202 GACGCC G[GA]^CG[CT]C 466 GACGCC BsmI 598 GCATTC GAATGC(1/-1) 935 GCATTC BsoBI 220 CTCGAG C^[CT]CG[GA]G 606 CCCGGG EaeI 26 CGGCCG [CT]^GGCC[GA] 276 TGGCCA EarI 622 GAAGAG CTCTTC(1/4) 987 GAAGAG HgaI 202 GACGC GACGC(5/10) 466 GACGC Hin1I 202 GACGCC G[GA]^CG[CT]C 466 GACGCC Ksp632I 622 GAAGAG CTCTTC(1/4) 987 GAAGAG MaeIII 185 GTGAC ^GT[ACGT]AC 563 GTCAC NspI 308 ACATGC [GA]CATG^[CT] 573 ACATGT PleI 900 GAGTC GAGTC(4/5) 1016 GAGTC PvuII 349 CAGCTG CAG^CTG 745 CAGCTG SfaNI 908 GCATC GCATC(5/9) 944 GCATC SfcI 160 CTGCAG C^T[GA][CT]AG 336 CTACAG TaqI 221 TCGA T^CGA 839 TCGA Tsp45I 185 GTGAC ^GT[GC]AC 563 GTCAC TthHB8I 221 TCGA T^CGA 839 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BbvI BcnI BmyI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI