(Circular) (Six-base) MAPSORT of: pFN21AE4343.seq Check: 6633 from: 1 to: 5767 pFN21AE4343 5767 bp With 182 enzymes: SgfI * March 28, 2012 13:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2370 2370 Size: 5767 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3577 278 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 3110 1937 Size: 922 251 4594 Fragments arranged by size: 4594 922 251 Acc65I G'GTAC_C Cuts at: 3088 3088 Size: 5767 AclI AA'CG_TT Cuts at: 1563 3894 4267 1563 Size: 2331 373 3063 Fragments arranged by size: 3063 2331 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2548 3830 5214 5482 1632 Size: 916 1282 1384 268 1917 Fragments arranged by size: 1917 1384 1282 916 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5570 AflIII A'CryG_T Cuts at: 1203 2578 4666 5643 1203 Size: 1375 2088 977 1327 Fragments arranged by size: 2088 1375 1327 977 AgeI A'CCGG_T Cuts at: 4598 4598 Size: 5767 AhdI GACnn_n'nnGTC Cuts at: 4496 4496 Size: 5767 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5767 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5767 AlwNI CAG_nnn'CTG Cuts at: 2755 3510 5082 2755 Size: 755 1572 3440 Fragments arranged by size: 3440 1572 755 ApaLI G'TGCA_C Cuts at: 3824 4980 3824 Size: 1156 4611 ApoI r'AATT_y Cuts at: 1493 2736 3076 3302 3454 5563 1493 Size: 1243 340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1243 340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4321 160 Size: 872 3289 1606 Fragments arranged by size: 3289 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2611 3092 1870 Size: 73 282 386 481 4545 Fragments arranged by size: 4545 481 386 282 73 BamHI G'GATC_C Cuts at: 3097 3464 3097 Size: 367 5400 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3088 4543 618 Size: 325 217 627 1301 1455 1842 Fragments arranged by size: 1842 1455 1301 627 325 217 BanII G_rGCy'C Cuts at: 1391 2537 3086 1391 Size: 1146 549 4072 Fragments arranged by size: 4072 1146 549 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4796 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3958 3992 1903 Size: 34 50 34 1937 34 3678 Fragments arranged by size: 3678 1937 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3659 4869 3659 Size: 1210 4557 BclI T'GATC_A Cuts at: 1548 2814 1548 Size: 1266 4501 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4378 136 Size: 107 122 71 631 605 2706 1525 Fragments arranged by size: 2706 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2559 5762 2559 Size: 3203 2564 BlpI GC'TnA_GC Cuts at: 3184 3184 Size: 5767 Bme1580I G_kGCm'C Cuts at: 1790 3828 4984 1790 Size: 2038 1156 2573 Fragments arranged by size: 2573 2038 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5767 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 2377 3498 Size: 416 53 83 399 24 658 302 1121 Cuts at: 3498 4456 442 Size: 958 1753 Fragments arranged by size: 1753 1121 958 658 416 399 302 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5767 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 5767 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 2315 4427 1239 Size: 944 132 2112 2579 Fragments arranged by size: 2579 2112 944 132 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3892 4757 5055 3892 Size: 865 298 4604 Fragments arranged by size: 4604 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4430 882 Size: 617 2931 2219 Fragments arranged by size: 2931 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3558 493 Size: 711 2354 2702 Fragments arranged by size: 2702 2354 711 BsaBI GATnn'nnATC Cuts at: 5761 5761 Size: 5767 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 2472 3574 3956 Size: 53 83 186 936 675 264 1102 382 Cuts at: 3956 4591 275 Size: 635 1451 Fragments arranged by size: 1451 1102 936 675 635 382 264 186 83 53 BsaWI w'CCGG_w Cuts at: 2638 4200 4598 4872 5019 5430 2638 Size: 1562 398 274 147 411 2975 Fragments arranged by size: 2975 1562 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5542 Fragments arranged by size: 5542 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5767 BseYI C'CCAG_C Cuts at: 4970 5466 4970 Size: 496 5271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2006 2168 2342 2348 2420 2764 1163 Size: 573 270 162 174 6 72 344 4166 Fragments arranged by size: 4166 573 344 270 174 162 72 6 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3217 3978 5006 1540 Size: 275 186 33 1183 761 1028 2301 Fragments arranged by size: 2301 1183 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 2040 2403 2709 3828 3913 4984 2040 Size: 363 306 1119 85 1071 2823 Fragments arranged by size: 2823 1119 1071 363 306 85 BsmI GAATG_Cn' Cuts at: 2603 2940 3278 3371 2603 Size: 337 338 93 4999 Fragments arranged by size: 4999 338 337 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2403 2537 2709 3086 3828 3913 Size: 399 250 363 134 172 377 742 85 Cuts at: 3913 4984 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 377 363 250 172 134 85 BspEI T'CCGG_A Cuts at: 2638 5430 2638 Size: 2792 2975 BspHI T'CATG_A Cuts at: 3656 5386 3656 Size: 1730 4037 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2190 2310 2370 2821 3122 844 Size: 1346 120 60 451 301 3489 Fragments arranged by size: 3489 1346 451 301 120 60 BsrBI CCG'CTC Cuts at: 1394 1823 3654 1394 Size: 429 1831 3507 Fragments arranged by size: 3507 1831 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4262 4436 65 Size: 1183 3014 174 1396 Fragments arranged by size: 3014 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4411 4598 1700 Size: 110 2601 187 2869 Fragments arranged by size: 2869 2601 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5767 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5767 BssSI C'ACGA_G Cuts at: 3827 4839 3827 Size: 1012 4755 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5767 BstBI TT'CG_AA Cuts at: 4571 4571 Size: 5767 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5767 BstXI CCAn_nnnn'nTGG Cuts at: 3046 3046 Size: 5767 BstYI r'GATC_y Cuts at: 1972 2559 3097 3464 3849 3866 5318 5404 5416 Size: 587 538 367 385 17 1452 86 12 Cuts at: 5416 5762 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 587 538 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2860 2860 Size: 5767 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5028 Fragments arranged by size: 5028 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3283 4077 4097 2478 Size: 805 794 20 4148 Fragments arranged by size: 4148 805 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5767 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5732 DraI TTT'AAA Cuts at: 3071 3071 Size: 5767 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4774 809 Size: 507 3458 1802 Fragments arranged by size: 3458 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2281 3214 Size: 54 1425 50 275 18 201 250 933 Cuts at: 3214 4103 5732 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 933 889 275 250 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3214 1537 Size: 275 219 1183 4090 Fragments arranged by size: 4090 1183 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2622 2987 3697 1353 Size: 1269 365 710 3423 Fragments arranged by size: 3423 1269 710 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4354 4728 4874 387 Size: 1064 138 2765 374 146 1280 Fragments arranged by size: 2765 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2315 2548 3830 4427 5214 5482 Size: 393 551 132 233 1282 597 787 268 Cuts at: 5482 1239 Size: 1524 Fragments arranged by size: 1524 1282 787 597 551 393 268 233 132 EcoO109I rG'GnC_Cy Cuts at: 2158 2326 2673 2158 Size: 168 347 5252 Fragments arranged by size: 5252 347 168 EcoRI G'AATT_C Cuts at: 3076 3454 3076 Size: 378 5389 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2651 2651 Size: 5767 FspI TGC'GCA Cuts at: 4273 4273 Size: 5767 HaeII r_GCGC'y Cuts at: 1678 4914 1678 Size: 3236 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2551 2583 2799 2831 4408 Size: 32 135 32 979 32 216 32 1577 Cuts at: 4408 4440 4482 4514 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1577 979 216 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3111 3357 669 Size: 988 281 1173 246 3079 Fragments arranged by size: 3079 1173 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5767 HpaI GTT'AAC Cuts at: 3357 3357 Size: 5767 KpnI G_GTAC'C Cuts at: 3092 3092 Size: 5767 MfeI C'AATT_G Cuts at: 3366 3366 Size: 5767 MluI A'CGCG_T Cuts at: 5643 5643 Size: 5767 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4881 5065 1305 Size: 3576 184 2007 Fragments arranged by size: 3576 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2283 10 Size: 54 1425 343 451 3494 Fragments arranged by size: 3494 1425 451 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2786 3044 3725 4084 4243 518 Size: 648 1362 258 258 681 359 159 2042 Fragments arranged by size: 2042 1362 681 648 359 258 258 159 MspA1I CmG'CkG Cuts at: 1606 2356 2412 2651 2752 3183 3860 5008 5253 Size: 750 56 239 101 431 677 1148 245 Cuts at: 5253 1606 Size: 2120 Fragments arranged by size: 2120 1148 750 677 431 245 239 101 56 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5215 NdeI CA'TA_TG Cuts at: 387 387 Size: 5767 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5767 NotI GC'GGCC_GC Cuts at: 3214 3214 Size: 5767 NspI r_CATG'y Cuts at: 1132 1229 2317 2582 3125 4670 1132 Size: 97 1088 265 543 1545 2229 Fragments arranged by size: 2229 1545 1088 543 265 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5767 PciI A'CATG_T Cuts at: 2578 4666 2578 Size: 2088 3679 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5767 PmeI GTTT'AAAC Cuts at: 3071 3071 Size: 5767 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2323 3836 3868 5349 5381 2291 Size: 32 1513 32 1481 32 2677 Fragments arranged by size: 2677 1513 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2673 2673 Size: 5767 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5767 PsiI TTA'TAA Cuts at: 2451 3337 2451 Size: 886 4881 PspXI vC'TCGA_Gb Cuts at: 2225 2225 Size: 5767 PstI C_TGCA'G Cuts at: 830 2169 3119 830 Size: 1339 950 3478 Fragments arranged by size: 3478 1339 950 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5767 PvuII CAG'CTG Cuts at: 2356 2752 2356 Size: 396 5371 SalI G'TCGA_C Cuts at: 1936 3109 1936 Size: 1173 4594 SanDI GG'GwC_CC Cuts at: 2673 2673 Size: 5767 SapI GCTCTTCn'nnn_ Cuts at: 2622 2622 Size: 5767 SbfI CC_TGCA'GG Cuts at: 3119 3119 Size: 5767 ScaI AGT'ACT Cuts at: 4015 5727 4015 Size: 1712 4055 SfcI C'TryA_G Cuts at: 826 1045 2165 2341 3115 4250 4931 5122 826 Size: 219 1120 176 774 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 774 681 219 191 176 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5767 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5767 SmaI CCC'GGG Cuts at: 1872 2613 3094 1872 Size: 741 481 4545 Fragments arranged by size: 4545 741 481 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 3871 4772 5034 820 Size: 197 926 282 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 282 262 197 SnaBI TAC'GTA Cuts at: 493 3558 493 Size: 3065 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5767 SphI G_CATG'C Cuts at: 1132 3125 1132 Size: 1993 3774 SspI AAT'ATT Cuts at: 5 52 2657 3691 5 Size: 47 2605 1034 2081 Fragments arranged by size: 2605 2081 1034 47 StuI AGG'CCT Cuts at: 1517 2502 1517 Size: 985 4782 StyI C'CwwG_G Cuts at: 513 1065 2233 3206 513 Size: 552 1168 973 3074 Fragments arranged by size: 3074 1168 973 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3794 3811 3964 4149 4594 325 Size: 1255 2214 17 153 185 445 1498 Fragments arranged by size: 2214 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2586 2775 2888 Size: 274 80 33 51 1443 609 189 113 Cuts at: 2888 4013 5725 96 Size: 1125 1712 138 Fragments arranged by size: 1712 1443 1125 609 274 189 138 113 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5727 5759 5727 Size: 32 5735 Tth111I GACn'n_nGTC Cuts at: 1316 2904 1316 Size: 1588 4179 XbaI T'CTAG_A Cuts at: 3103 3103 Size: 5767 XhoI C'TCGA_G Cuts at: 1943 2225 1943 Size: 282 5485 XmaI C'CCGG_G Cuts at: 1870 2611 3092 1870 Size: 741 481 4545 Fragments arranged by size: 4545 741 481 XmnI GAAnn'nnTTC Cuts at: 3896 3896 Size: 5767 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3575 276 Size: 53 83 186 936 2041 2468 Fragments arranged by size: 2468 2041 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI