################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BamHI BbeI BbrPI BcgI BciVI BfrI BlnI BsaHI BsiWI BsmBI BsmI Bsp1407I BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III EcoO109I EcoO65I EcoRV FseI FspI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MunI NarI NcoI NdeI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 1307 GTAGAC GT^[AC][GT]AC AccII 521 CGCG CG^CG BanI 590 GGTGCC G^G[CT][GA]CC BclI 120 TGATCA T^GATCA BglI 1390 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1529 AGATCT A^GATCT BsaAI 493 TACGTG [CT]AC^GT[GA] BsaBI 465 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1084 GAGACC GGTCTC(1/5) BsgI 1218 CTGCAC GTGCAG(16/14) BspDI 1630 ATCGAT AT^CGAT BspHI 515 TCATGA T^CATGA BsrDI 933 CATTGC GCAATG(2/0) BsrFI 755 GCCGGC [GA]^CCGG[CT] BstUI 521 CGCG CG^CG CfoI 857 GCGC GCG^C Cfr10I 755 GCCGGC [GA]^CCGG[CT] ClaI 1630 ATCGAT AT^CGAT EagI 842 CGGCCG C^GGCCG EarI 184 GAAGAG CTCTTC(1/4) EclXI 842 CGGCCG C^GGCCG Eco52I 842 CGGCCG C^GGCCG EcoNI 66 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1747 GAATTC G^AATTC EcoT22I 1426 ATGCAT ATGCA^T FbaI 120 TGATCA T^GATCA HaeII 856 AGCGCC [GA]GCGC^[CT] HhaI 857 GCGC GCG^C HinP1I 857 GCGC G^CGC Ksp632I 184 GAAGAG CTCTTC(1/4) MamI 465 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 967 TTAA T^TAA MvnI 521 CGCG CG^CG NaeI 755 GCCGGC GCC^GGC NgoMIV 755 GCCGGC G^CCGGC NheI 1128 GCTAGC G^CTAGC NotI 841 GCGGCCGC GC^GGCCGC NsiI 1426 ATGCAT ATGCA^T PstI 1400 CTGCAG CTGCA^G RcaI 515 TCATGA T^CATGA SacI 1173 GAGCTC GAGCT^C SfcI 1400 CTGCAG C^T[GA][CT]AG SmaI 901 CCCGGG CCC^GGG SphI 506 GCATGC GCATG^C TfiI 484 GATTC G^A[AT]TC Tru9I 967 TTAA T^TAA XmaCI 901 CCCGGG C^CCGGG XmaI 901 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccIII 1266 TCCGGA T^CCGGA 1550 TCCGGA AcsI 1570 AAATTC [GA]^AATT[CT] 1747 GAATTC AlwNI 855 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1394 CAGCGGCTG AocI 195 CCTCAGG CC^T[ACGT]AGG 817 CCTGAGG ApoI 1570 AAATTC [GA]^AATT[CT] 1747 GAATTC Asp700I 369 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1270 GAAGGTGTTC AspHI 787 GAGCAC G[AT]GC[AT]^C 1173 GAGCTC BanII 447 GGGCTC G[GA]GC[CT]^C 1173 GAGCTC BbvCI 7 CCTCAGC CCTCAGC(-5/-2) 327 CCTCAGC BfaI 1129 CTAG C^TAG 1327 CTAG BlpI 103 GCTGAGC GC^T[ACGT]AGC 1491 GCTTAGC Bpu1102I 103 GCTGAGC GC^T[ACGT]AGC 1491 GCTTAGC BseAI 1266 TCCGGA T^CCGGA 1550 TCCGGA BsiEI 339 CGGTCG CG[GA][CT]^CG 842 CGGCCG BsiHKAI 787 GAGCAC G[AT]GC[AT]^C 1173 GAGCTC BsmFI 1012 GGGAC GGGAC(10/14) 1321 GTCCC BspEI 1266 TCCGGA T^CCGGA 1550 TCCGGA BspMI 662 ACCTGC ACCTGC(4/8) 1311 ACCTGC BstYI 1529 AGATCT [GA]^GATC[CT] 1832 AGATCC Bsu36I 195 CCTCAGG CC^T[ACGT]AGG 817 CCTGAGG CelII 103 GCTGAGC GC^T[ACGT]AGC 1491 GCTTAGC Eco81I 195 CCTCAGG CC^T[ACGT]AGG 817 CCTGAGG FauI 707 GCGGG CCCGC(4/6) 863 GCGGG HincII 619 GTCAAC GT[CT]^[GA]AC 1540 GTCAAC HindII 619 GTCAAC GT[CT]^[GA]AC 1540 GTCAAC MaeI 1129 CTAG C^TAG 1327 CTAG MflI 1529 AGATCT [GA]^GATC[CT] 1832 AGATCC MroI 1266 TCCGGA T^CCGGA 1550 TCCGGA MslI 130 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1713 CATCAACATG NspI 506 GCATGC [GA]CATG^[CT] 791 ACATGC PaeR7I 91 CTCGAG C^TCGAG 1209 CTCGAG PleI 1261 GAGTC GAGTC(4/5) 1358 GAGTC PvuII 10 CAGCTG CAG^CTG 1344 CAGCTG SmlI 91 CTCGAG C^T[CT][GA]AG 1209 CTCGAG StuI 324 AGGCCT AGG^CCT 1701 AGGCCT Tsp509I 1571 AATT ^AATT 1748 AATT XhoI 91 CTCGAG C^TCGAG 1209 CTCGAG XhoII 1529 AGATCT [GA]^GATC[CT] 1832 AGATCC XmnI 369 GAAAGCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1270 GAAGGTGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AfaI AluI AlwI AvaI BalI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I