################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## Acc65I AccIII AciI AclI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BstAPI BstBI BstF5I Cac8I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI HindIII HinfI HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I TspRI Tth111I TthHB8I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 313 GACGTC GACGT^C AccI 413 GTATAC GT^[AC][GT]AC AccII 310 CGCG CG^CG AcsI 324 AAATTT [GA]^AATT[CT] AcyI 313 GACGTC G[GA]^CG[CT]C AfaI 81 GTAC GT^AC AluI 182 AGCT AG^CT AocI 402 CCTCAGG CC^T[ACGT]AGG ApoI 324 AAATTT [GA]^AATT[CT] Asp700I 257 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 278 CCCGGG C^[CT]CG[GA]G BbsI 58 GTCTTC GAAGAC(2/6) BfaI 347 CTAG C^TAG BglII 389 AGATCT A^GATCT BpuAI 58 GTCTTC GAAGAC(2/6) BsaHI 313 GACGTC G[GA]^CG[CT]C BsmFI 428 GTCCC GGGAC(10/14) BsoBI 278 CCCGGG C^[CT]CG[GA]G BspHI 329 TCATGA T^CATGA BspMI 104 ACCTGC ACCTGC(4/8) BssSI 364 CTCGTG CACGAG(-5/-1) Bst1107I 413 GTATAC GTA^TAC BstEII 157 GGTGACC G^GT[ACGT]ACC BstPI 157 GGTGACC G^GT[ACGT]ACC BstUI 310 CGCG CG^CG BstXI 32 CCATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 389 AGATCT [GA]^GATC[CT] BstZ17I 413 GTATAC GTA^TAC Bsu36I 402 CCTCAGG CC^T[ACGT]AGG CfoI 309 GCGC GCG^C DpnII 390 GATC ^GATC DrdI 291 GACGGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 368 TGGCCG [CT]^GGCC[GA] Eco81I 402 CCTCAGG CC^T[ACGT]AGG EcoO65I 157 GGTGACC G^GT[ACGT]ACC HhaI 309 GCGC GCG^C Hin1I 313 GACGTC G[GA]^CG[CT]C HinP1I 309 GCGC G^CGC HincII 165 GTTGAC GT[CT]^[GA]AC HindII 165 GTTGAC GT[CT]^[GA]AC HphI 157 GGTGA GGTGA(8/7) MaeI 347 CTAG C^TAG MaeII 314 ACGT A^CGT MboI 390 GATC ^GATC MflI 389 AGATCT [GA]^GATC[CT] MslI 103 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 310 CGCG CG^CG NdeII 390 GATC ^GATC PshAI 210 GACGATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 318 CTGCAG CTGCA^G RcaI 329 TCATGA T^CATGA RsaI 81 GTAC GT^AC Sau3AI 390 GATC ^GATC ScaI 80 AGTACT AGT^ACT SmaI 278 CCCGGG CCC^GGG Tsp509I 325 AATT ^AATT XbaI 346 TCTAGA T^CTAGA XhoII 389 AGATCT [GA]^GATC[CT] XmaCI 278 CCCGGG C^CCGGG XmaI 278 CCCGGG C^CCGGG XmnI 257 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AlwNI 178 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG 198 CAGGACCTG ApaI 11 GGGCCC GGGCC^C 147 GGGCCC BbvI 28 GCAGC GCAGC(8/12) 271 GCTGC BcnI 278 CCCGG CC^[GC]GG 279 CCGGG BseRI 115 GAGGAG GAGGAG(10/8) 333 GAGGAG EcoNI 135 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 418 CCTTCTACAGG Fnu4HI 28 GCAGC GC^[ACGT]GC 271 GCTGC HapII 279 CCGG C^CGG 371 CCGG HpaII 279 CCGG C^CGG 371 CCGG ItaI 28 GCAGC GC^[ACGT]GC 271 GCTGC MspI 279 CCGG C^CGG 371 CCGG MwoI 4 GCTACCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 311 GCGACGTCTGC NciI 278 CCCGG CC^[GC]GG 279 CCGGG NlaIII 33 CATG CATG^ 330 CATG TseI 28 GCAGC G^C[AT]GC 271 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AvaII BanII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DdeI DraII EcoO109I EcoRII HaeIII MaeIII MboII MnlI MvaI NlaIV PpuMI Sau96I ScrFI SfcI Tsp45I