(Circular) (Six-base) MAPSORT of: pFN21AE5118.seq Check: 2484 from: 1 to: 5140 pFN21AE5118 5140 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2118 2118 Size: 5140 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2322 2950 278 Size: 53 83 186 936 786 628 2468 Fragments arranged by size: 2468 936 786 628 186 83 53 AccI GT'mk_AC Cuts at: 1937 2419 2483 1937 Size: 482 64 4594 Fragments arranged by size: 4594 482 64 Acc65I G'GTAC_C Cuts at: 2461 2461 Size: 5140 AclI AA'CG_TT Cuts at: 1563 3267 3640 1563 Size: 1704 373 3063 Fragments arranged by size: 3063 1704 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2252 3203 4587 4855 1632 Size: 620 951 1384 268 1917 Fragments arranged by size: 1917 1384 951 620 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4943 AflIII A'CryG_T Cuts at: 1203 4039 5016 1203 Size: 2836 977 1327 Fragments arranged by size: 2836 1327 977 AgeI A'CCGG_T Cuts at: 3971 3971 Size: 5140 AhdI GACnn_n'nnGTC Cuts at: 3869 3869 Size: 5140 AleI CACnn'nnGTG Cuts at: 1166 2112 1166 Size: 946 4194 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5140 AlwNI CAG_nnn'CTG Cuts at: 2188 2208 2883 4455 2188 Size: 20 675 1572 2873 Fragments arranged by size: 2873 1572 675 20 ApaI G_GGCC'C Cuts at: 2020 2156 2020 Size: 136 5004 ApaLI G'TGCA_C Cuts at: 3197 4353 3197 Size: 1156 3984 ApoI r'AATT_y Cuts at: 1493 2329 2449 2675 2827 4936 1493 Size: 836 120 226 152 2109 1697 Fragments arranged by size: 2109 1697 836 226 152 120 AseI AT'TA_AT Cuts at: 160 1032 3694 160 Size: 872 2662 1606 Fragments arranged by size: 2662 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2465 1870 Size: 73 340 182 4545 Fragments arranged by size: 4545 340 182 73 BamHI G'GATC_C Cuts at: 2470 2837 2470 Size: 367 4773 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2461 3916 618 Size: 325 217 627 674 1455 1842 Fragments arranged by size: 1842 1455 674 627 325 217 BanII G_rGCy'C Cuts at: 1391 2020 2081 2107 2156 2459 1391 Size: 629 61 26 49 303 4072 Fragments arranged by size: 4072 629 303 61 49 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2056 928 Size: 971 157 4012 Fragments arranged by size: 4012 971 157 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3331 3365 1903 Size: 34 1394 34 3678 Fragments arranged by size: 3678 1394 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3032 4242 3032 Size: 1210 3930 BclI T'GATC_A Cuts at: 1548 1548 Size: 5140 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3751 136 Size: 107 122 71 631 605 2079 1525 Fragments arranged by size: 2079 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2394 5135 2394 Size: 2741 2399 BlpI GC'TnA_GC Cuts at: 2557 2557 Size: 5140 Bme1580I G_kGCm'C Cuts at: 1790 2020 2156 3201 4357 1790 Size: 230 136 1045 1156 2573 Fragments arranged by size: 2573 1156 1045 230 136 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5140 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2871 3829 442 Size: 416 53 83 399 24 1454 958 1753 Fragments arranged by size: 1753 1454 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5140 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3800 1239 Size: 2561 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3265 4130 4428 3265 Size: 865 298 3977 Fragments arranged by size: 3977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3803 882 Size: 617 2304 2219 Fragments arranged by size: 2304 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2931 493 Size: 711 1727 2702 Fragments arranged by size: 2702 1727 711 BsaBI GATnn'nnATC Cuts at: 5134 5134 Size: 5140 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2319 2947 3329 3964 Size: 53 83 186 936 786 628 382 635 Cuts at: 3964 275 Size: 1451 Fragments arranged by size: 1451 936 786 635 628 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3573 3971 4245 4392 4803 3573 Size: 398 274 147 411 3910 Fragments arranged by size: 3910 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4915 Fragments arranged by size: 4915 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2353 1186 Size: 949 218 3973 Fragments arranged by size: 3973 949 218 BseYI C'CCAG_C Cuts at: 2188 2388 4343 4839 2188 Size: 200 1955 496 2489 Fragments arranged by size: 2489 1955 496 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4567 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2590 3351 4379 1540 Size: 275 186 589 761 1028 2301 Fragments arranged by size: 2301 1028 761 589 275 186 BsiHKAI G_wGCw'C Cuts at: 3201 3286 4357 3201 Size: 85 1071 3984 Fragments arranged by size: 3984 1071 85 BsmI GAATG_Cn' Cuts at: 2651 2744 2651 Size: 93 5047 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2081 2107 2156 2459 3201 3286 Size: 399 230 61 26 49 303 742 85 Cuts at: 3286 4357 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 230 85 61 49 26 BspEI T'CCGG_A Cuts at: 4803 4803 Size: 5140 BspHI T'CATG_A Cuts at: 2334 3029 4759 2334 Size: 695 1730 2715 Fragments arranged by size: 2715 1730 695 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 2495 844 Size: 1274 377 3489 Fragments arranged by size: 3489 1274 377 BsrBI CCG'CTC Cuts at: 1394 1823 3027 1394 Size: 429 1204 3507 Fragments arranged by size: 3507 1204 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3635 3809 65 Size: 1183 2387 174 1396 Fragments arranged by size: 2387 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3784 3971 1700 Size: 110 1974 187 2869 Fragments arranged by size: 2869 1974 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5140 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5140 BssSI C'ACGA_G Cuts at: 2369 3200 4212 2369 Size: 831 1012 3297 Fragments arranged by size: 3297 1012 831 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5140 BstBI TT'CG_AA Cuts at: 3944 3944 Size: 5140 BstEII G'GTnAC_C Cuts at: 1184 2162 1184 Size: 978 4162 BstXI CCAn_nnnn'nTGG Cuts at: 2044 2044 Size: 5140 BstYI r'GATC_y Cuts at: 1972 2394 2470 2837 3222 3239 4691 4777 4789 Size: 422 76 367 385 17 1452 86 12 Cuts at: 4789 5135 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 422 385 367 346 86 76 17 12 BstZ17I GTA'TAC Cuts at: 2420 2420 Size: 5140 Bsu36I CC'TnA_GG Cuts at: 2408 2408 Size: 5140 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3649 Fragments arranged by size: 3649 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4401 Fragments arranged by size: 4401 683 56 BtsI GCAGTG_nn' Cuts at: 2656 3450 3470 2656 Size: 794 20 4326 Fragments arranged by size: 4326 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5140 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5105 DraI TTT'AAA Cuts at: 2444 2444 Size: 5140 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2302 4147 809 Size: 507 986 1845 1802 Fragments arranged by size: 1845 1802 986 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2373 2587 3476 Size: 54 1425 50 275 18 543 214 889 Cuts at: 3476 5105 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 543 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2587 1537 Size: 275 775 4090 Fragments arranged by size: 4090 775 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3070 1353 Size: 1717 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3727 4101 4247 387 Size: 1064 138 2138 374 146 1280 Fragments arranged by size: 2138 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2252 3203 3800 4587 4855 1239 Size: 393 620 951 597 787 268 1524 Fragments arranged by size: 1524 951 787 620 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2144 2427 2144 Size: 283 4857 EcoO109I rG'GnC_Cy Cuts at: 2017 2152 2153 2205 2365 2404 2432 2017 Size: 135 1 52 160 39 28 4725 Fragments arranged by size: 4725 160 135 52 39 28 1 EcoRI G'AATT_C Cuts at: 2449 2827 2449 Size: 378 4762 FspI TGC'GCA Cuts at: 3646 3646 Size: 5140 HaeII r_GCGC'y Cuts at: 1678 4287 1678 Size: 2609 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 2329 2361 3781 Size: 32 135 32 539 32 186 32 1420 Cuts at: 3781 3813 3855 3887 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1420 539 186 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2172 2484 2730 669 Size: 988 281 234 312 246 3079 Fragments arranged by size: 3079 988 312 281 246 234 HindIII A'AGCT_T Cuts at: 748 748 Size: 5140 HpaI GTT'AAC Cuts at: 2730 2730 Size: 5140 KpnI G_GTAC'C Cuts at: 2465 2465 Size: 5140 MfeI C'AATT_G Cuts at: 2739 2739 Size: 5140 MluI A'CGCG_T Cuts at: 5016 5016 Size: 5140 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4254 4438 1305 Size: 2949 184 2007 Fragments arranged by size: 2949 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3318 Fragments arranged by size: 3318 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2112 3098 3457 3616 518 Size: 648 946 986 359 159 2042 Fragments arranged by size: 2042 986 946 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2556 3233 4381 4626 1606 Size: 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 950 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3649 Fragments arranged by size: 3649 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5140 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5140 NotI GC'GGCC_GC Cuts at: 2587 2587 Size: 5140 NspI r_CATG'y Cuts at: 1132 1229 2498 4043 1132 Size: 97 1269 1545 2229 Fragments arranged by size: 2229 1545 1269 97 PasI CC'CwG_GG Cuts at: 1348 2158 2362 1348 Size: 810 204 4126 Fragments arranged by size: 4126 810 204 PciI A'CATG_T Cuts at: 4039 4039 Size: 5140 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5140 PmeI GTTT'AAAC Cuts at: 2444 2444 Size: 5140 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3209 3241 4722 4754 3209 Size: 32 1481 32 3595 Fragments arranged by size: 3595 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2205 2404 2432 2205 Size: 199 28 4913 Fragments arranged by size: 4913 199 28 PshAI GACnn'nnGTC Cuts at: 1613 2219 1613 Size: 606 4534 PsiI TTA'TAA Cuts at: 2710 2710 Size: 5140 PspOMI G'GGCC_C Cuts at: 2016 2152 2016 Size: 136 5004 PstI C_TGCA'G Cuts at: 830 2327 2492 830 Size: 1497 165 3478 Fragments arranged by size: 3478 1497 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5140 SalI G'TCGA_C Cuts at: 1936 2482 1936 Size: 546 4594 SbfI CC_TGCA'GG Cuts at: 2492 2492 Size: 5140 ScaI AGT'ACT Cuts at: 2087 3388 5100 2087 Size: 1301 1712 2127 Fragments arranged by size: 2127 1712 1301 SfcI C'TryA_G Cuts at: 826 1045 2200 2323 2427 2488 3623 4304 4495 Size: 219 1155 123 104 61 1135 681 191 Cuts at: 4495 826 Size: 1471 Fragments arranged by size: 1471 1155 1135 681 219 191 123 104 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5140 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5140 SmaI CCC'GGG Cuts at: 1872 2285 2467 1872 Size: 413 182 4545 Fragments arranged by size: 4545 413 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 3244 4145 4407 820 Size: 197 926 1301 901 262 1553 Fragments arranged by size: 1553 1301 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2931 493 Size: 2438 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5140 SphI G_CATG'C Cuts at: 1132 2498 1132 Size: 1366 3774 SspI AAT'ATT Cuts at: 5 52 3064 5 Size: 47 3012 2081 Fragments arranged by size: 3012 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5140 StyI C'CwwG_G Cuts at: 513 1065 2004 2579 513 Size: 552 939 575 3074 Fragments arranged by size: 3074 939 575 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 3167 3184 3337 3522 3967 325 Size: 1255 690 897 17 153 185 445 1498 Fragments arranged by size: 1498 1255 897 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 3386 5098 Size: 274 80 33 51 1443 108 1301 1712 Cuts at: 5098 96 Size: 138 Fragments arranged by size: 1712 1443 1301 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5100 5132 5100 Size: 32 5108 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5140 XbaI T'CTAG_A Cuts at: 2351 2476 2351 Size: 125 5015 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5140 XmaI C'CCGG_G Cuts at: 1870 2283 2465 1870 Size: 413 182 4545 Fragments arranged by size: 4545 413 182 XmnI GAAnn'nnTTC Cuts at: 2266 3269 2266 Size: 1003 4137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2320 2948 276 Size: 53 83 186 936 786 628 2468 Fragments arranged by size: 2468 936 786 628 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI