(Linear) (Six-base) MAPSORT of: KIEE5118.seq Check: 817 from: 1 to: 435 KIEE5118.orf With 182 enzymes: SgfI * January 29, 2014 11:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 113 435 Size: 113 322 AatII G_ACGT'C Cuts at: 0 317 435 Size: 317 118 AccI GT'mk_AC Cuts at: 0 414 435 Size: 414 21 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 435 Size: 247 188 AleI CACnn'nnGTG Cuts at: 0 107 435 Size: 107 328 AlwNI CAG_nnn'CTG Cuts at: 0 183 203 435 Size: 183 20 232 Fragments arranged by size: 232 183 20 ApaI G_GGCC'C Cuts at: 0 15 151 435 Size: 15 136 284 Fragments arranged by size: 284 136 15 ApoI r'AATT_y Cuts at: 0 324 435 Size: 324 111 AvaI C'yCGr_G Cuts at: 0 278 435 Size: 278 157 BanII G_rGCy'C Cuts at: 0 15 76 102 151 435 Size: 15 61 26 49 284 Fragments arranged by size: 284 61 49 26 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 51 435 Size: 51 384 BglII A'GATC_T Cuts at: 0 389 435 Size: 389 46 Bme1580I G_kGCm'C Cuts at: 0 15 151 435 Size: 15 136 284 Fragments arranged by size: 284 136 15 BsaHI Gr'CG_yC Cuts at: 0 314 435 Size: 314 121 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 130 348 435 Size: 130 218 87 Fragments arranged by size: 218 130 87 BseYI C'CCAG_C Cuts at: 0 183 383 435 Size: 183 200 52 Fragments arranged by size: 200 183 52 Bsp1286I G_dGCh'C Cuts at: 0 15 76 102 151 435 Size: 15 61 26 49 284 Fragments arranged by size: 284 61 49 26 15 BspHI T'CATG_A Cuts at: 0 329 435 Size: 329 106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 113 435 Size: 113 322 BssSI C'ACGA_G Cuts at: 0 364 435 Size: 364 71 BstEII G'GTnAC_C Cuts at: 0 157 435 Size: 157 278 BstXI CCAn_nnnn'nTGG Cuts at: 0 39 435 Size: 39 396 BstYI r'GATC_y Cuts at: 0 389 435 Size: 389 46 BstZ17I GTA'TAC Cuts at: 0 415 435 Size: 415 20 Bsu36I CC'TnA_GG Cuts at: 0 403 435 Size: 403 32 DrdI GACnn_nn'nnGTC Cuts at: 0 297 435 Size: 297 138 EaeI y'GGCC_r Cuts at: 0 368 435 Size: 368 67 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 247 435 Size: 247 188 EcoNI CCTnn'n_nnAGG Cuts at: 0 139 422 435 Size: 139 283 13 Fragments arranged by size: 283 139 13 EcoO109I rG'GnC_Cy Cuts at: 0 12 147 148 200 360 399 427 435 Size: 12 135 1 52 160 39 28 8 Fragments arranged by size: 160 135 52 39 28 12 8 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 106 138 324 356 435 Size: 106 32 186 32 79 Fragments arranged by size: 186 106 79 32 32 HincII GTy'rAC Cuts at: 0 167 435 Size: 167 268 MslI CAynn'nnrTG Cuts at: 0 107 435 Size: 107 328 PasI CC'CwG_GG Cuts at: 0 153 357 435 Size: 153 204 78 Fragments arranged by size: 204 153 78 PpuMI rG'GwC_Cy Cuts at: 0 200 399 427 435 Size: 200 199 28 8 Fragments arranged by size: 200 199 28 8 PshAI GACnn'nnGTC Cuts at: 0 214 435 Size: 214 221 PspOMI G'GGCC_C Cuts at: 0 11 147 435 Size: 11 136 288 Fragments arranged by size: 288 136 11 PstI C_TGCA'G Cuts at: 0 322 435 Size: 322 113 ScaI AGT'ACT Cuts at: 0 82 435 Size: 82 353 SfcI C'TryA_G Cuts at: 0 195 318 422 435 Size: 195 123 104 13 Fragments arranged by size: 195 123 104 13 SmaI CCC'GGG Cuts at: 0 280 435 Size: 280 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 435 Size: 265 170 TatI w'GTAC_w Cuts at: 0 80 435 Size: 80 355 XbaI T'CTAG_A Cuts at: 0 346 435 Size: 346 89 XmaI C'CCGG_G Cuts at: 0 278 435 Size: 278 157 XmnI GAAnn'nnTTC Cuts at: 0 261 435 Size: 261 174 ZraI GAC'GTC Cuts at: 0 315 435 Size: 315 120 Enzymes that do cut: AarI AatII AccI AcuI AleI AlwNI ApaI ApoI AvaI BanII BbsI BglII Bme1580I BsaHI BseRI BseYI Bsp1286I BspHI BspMI BssSI BstEII BstXI BstYI BstZ17I Bsu36I DrdI EaeI Eco57MI EcoNI EcoO109I Hin4I HincII MslI PasI PpuMI PshAI PspOMI PstI ScaI SfcI SmaI TaqII TatI XbaI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BtgI BtgZI BtsI ClaI CspCI DraI DraIII EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TstI Tth111I XcmI XhoI