################################## # NO CUT ENZYMES ( 182 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AciI 618 GCGG CCGC(-3/-1) AfaI 655 GTAC GT^AC AvaII 271 GGACC G^G[AT]CC BamHI 550 GGATCC G^GATCC BfaI 595 CTAG C^TAG BglI 538 GCCAGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 155 AGATCT A^GATCT BsaI 25 GAGACC GGTCTC(1/5) BsmAI 25 GAGAC GTCTC(1/5) BsmFI 145 GGGAC GGGAC(10/14) BsmI 362 GCATTC GAATGC(1/-1) BsrI 539 CCAGT ACTGG(1/-1) BstXI 329 CCAAGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 271 GGACC G^G[ACGT]CC DdeI 367 CTGAG C^T[ACGT]AG DraII 270 AGGACCC [GA]G^G[ACGT]CC[CT] DsaI 515 CCACGG C^C[GA][CT]GG EcoO109I 270 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 586 GAATTC G^AATTC HaeIII 417 GGCC GG^CC HincII 324 GTTGAC GT[CT]^[GA]AC HindII 324 GTTGAC GT[CT]^[GA]AC HindIII 331 AAGCTT A^AGCTT MaeI 595 CTAG C^TAG MseI 424 TTAA T^TAA NspI 692 GCATGT [GA]CATG^[CT] PleI 708 GACTC GAGTC(4/5) PpuMI 270 AGGACCC [GA]G^G[AT]CC[CT] PstI 439 CTGCAG CTGCA^G RsaI 655 GTAC GT^AC Sau96I 271 GGACC G^G[ACGT]CC SfcI 439 CTGCAG C^T[GA][CT]AG TfiI 502 GATTC G^A[AT]TC Tru9I 424 TTAA T^TAA Tsp45I 232 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 219 AAATTT [GA]^AATT[CT] 586 GAATTC ApoI 219 AAATTT [GA]^AATT[CT] 586 GAATTC BbvI 347 GCAGC GCAGC(8/12) 615 GCTGC BspHI 498 TCATGA T^CATGA 711 TCATGA BstYI 155 AGATCT [GA]^GATC[CT] 550 GGATCC Fnu4HI 347 GCAGC GC^[ACGT]GC 615 GCTGC HinfI 502 GATTC G^A[ACGT]TC 708 GACTC ItaI 347 GCAGC GC^[ACGT]GC 615 GCTGC MaeIII 232 GTGAC ^GT[ACGT]AC 674 GTTAC MboII 111 GAAGA GAAGA(8/7) 727 GAAGA MflI 155 AGATCT [GA]^GATC[CT] 550 GGATCC NlaIV 271 GGACCC GG[ACGT]^[ACGT]CC 550 GGATCC RcaI 498 TCATGA T^CATGA 711 TCATGA SfaNI 564 GATGC GCATC(5/9) 199 GCATC TseI 347 GCAGC G^C[AT]GC 615 GCTGC XcmI 153 CCAGATCTTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 533 CCAAAGCCAGTCTGG XhoII 155 AGATCT [GA]^GATC[CT] 550 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AlwI AspHI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstNI DpnII EcoRII MboI MnlI MslI MvaI MwoI NdeII NlaIII Sau3AI ScrFI Tsp509I TspRI