(Circular) (Six-base) MAPSORT of: pFN21AE6231.seq Check: 2721 from: 1 to: 5449 pFN21AE6231 5449 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3259 278 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2792 1937 Size: 855 4594 Acc65I G'GTAC_C Cuts at: 2770 2770 Size: 5449 AclI AA'CG_TT Cuts at: 1563 3576 3949 1563 Size: 2013 373 3063 Fragments arranged by size: 3063 2013 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2135 3512 4896 5164 1632 Size: 503 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 503 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5252 AflIII A'CryG_T Cuts at: 1203 4348 5325 1203 Size: 3145 977 1327 Fragments arranged by size: 3145 1327 977 AgeI A'CCGG_T Cuts at: 4280 4280 Size: 5449 AhdI GACnn_n'nnGTC Cuts at: 4178 4178 Size: 5449 AleI CACnn'nnGTG Cuts at: 1166 2446 2615 1166 Size: 1280 169 4000 Fragments arranged by size: 4000 1280 169 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5449 AlwNI CAG_nnn'CTG Cuts at: 3192 4764 3192 Size: 1572 3877 ApaLI G'TGCA_C Cuts at: 3506 4662 3506 Size: 1156 4293 ApoI r'AATT_y Cuts at: 1493 2224 2591 2758 2984 3136 5245 1493 Size: 731 367 167 226 152 2109 1697 Fragments arranged by size: 2109 1697 731 367 226 167 152 AseI AT'TA_AT Cuts at: 160 1032 4003 160 Size: 872 2971 1606 Fragments arranged by size: 2971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 AvaI C'yCGr_G Cuts at: 1870 1943 2774 1870 Size: 73 831 4545 Fragments arranged by size: 4545 831 73 BamHI G'GATC_C Cuts at: 2555 2779 3146 2555 Size: 224 367 4858 Fragments arranged by size: 4858 367 224 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2770 4225 618 Size: 325 217 627 983 1455 1842 Fragments arranged by size: 1842 1455 983 627 325 217 BanII G_rGCy'C Cuts at: 1391 2768 1391 Size: 1377 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4478 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3640 3674 1903 Size: 34 1703 34 3678 Fragments arranged by size: 3678 1703 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3341 4551 3341 Size: 1210 4239 BclI T'GATC_A Cuts at: 1548 1548 Size: 5449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2549 4060 136 Size: 107 122 71 631 605 877 1511 1525 Fragments arranged by size: 1525 1511 877 631 605 122 107 71 BglII A'GATC_T Cuts at: 2160 5444 2160 Size: 3284 2165 BlpI GC'TnA_GC Cuts at: 2866 2866 Size: 5449 Bme1580I G_kGCm'C Cuts at: 1790 3510 4666 1790 Size: 1720 1156 2573 Fragments arranged by size: 2573 1720 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3180 4138 442 Size: 416 53 83 399 24 1763 958 1753 Fragments arranged by size: 1763 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4109 1239 Size: 2870 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3574 4439 4737 3574 Size: 865 298 4286 Fragments arranged by size: 4286 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2024 4112 882 Size: 617 525 2088 2219 Fragments arranged by size: 2219 2088 617 525 BsaAI yAC'GTr Cuts at: 493 1204 3240 493 Size: 711 2036 2702 Fragments arranged by size: 2702 2036 711 BsaBI GATnn'nnATC Cuts at: 5443 5443 Size: 5449 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3256 3638 4273 275 Size: 53 83 186 936 1723 382 635 1451 Fragments arranged by size: 1723 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3882 4280 4554 4701 5112 3882 Size: 398 274 147 411 4219 Fragments arranged by size: 4219 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5224 Fragments arranged by size: 5224 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5449 BseYI C'CCAG_C Cuts at: 4652 5148 4652 Size: 496 4953 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4876 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2899 3660 4688 1540 Size: 275 186 898 761 1028 2301 Fragments arranged by size: 2301 1028 898 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2380 2610 3510 3595 4666 2046 Size: 334 230 900 85 1071 2829 Fragments arranged by size: 2829 1071 900 334 230 85 BsmI GAATG_Cn' Cuts at: 2367 2960 3053 2367 Size: 593 93 4763 Fragments arranged by size: 4763 593 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2380 2610 2768 3510 3595 4666 Size: 399 256 334 230 158 742 85 1071 Cuts at: 4666 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 334 256 230 158 85 BspEI T'CCGG_A Cuts at: 5112 5112 Size: 5449 BspHI T'CATG_A Cuts at: 2503 2716 3338 5068 2503 Size: 213 622 1730 2884 Fragments arranged by size: 2884 1730 622 213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2804 844 Size: 1960 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3336 1394 Size: 429 1513 3507 Fragments arranged by size: 3507 1513 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3944 4118 65 Size: 1183 2696 174 1396 Fragments arranged by size: 2696 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4093 4280 1700 Size: 110 2283 187 2869 Fragments arranged by size: 2869 2283 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5449 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5449 BssSI C'ACGA_G Cuts at: 3509 4521 3509 Size: 1012 4437 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5449 BstBI TT'CG_AA Cuts at: 4253 4253 Size: 5449 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5449 BstXI CCAn_nnnn'nTGG Cuts at: 2341 2341 Size: 5449 BstYI r'GATC_y Cuts at: 1972 2160 2555 2779 3146 3531 3548 5000 5086 Size: 188 395 224 367 385 17 1452 86 Cuts at: 5086 5098 5444 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 395 385 367 346 224 188 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2520 513 Size: 552 1455 3442 Fragments arranged by size: 3442 1455 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4710 Fragments arranged by size: 4710 683 56 BtsI GCAGTG_nn' Cuts at: 2441 2453 2610 2965 3759 3779 2441 Size: 12 157 355 794 20 4111 Fragments arranged by size: 4111 794 355 157 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5414 DraI TTT'AAA Cuts at: 2753 2753 Size: 5449 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4456 809 Size: 507 3140 1802 Fragments arranged by size: 3140 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2896 3785 5414 Size: 54 1425 50 275 18 1066 889 1629 Cuts at: 5414 8 Size: 43 Fragments arranged by size: 1629 1425 1066 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2896 1537 Size: 275 1084 4090 Fragments arranged by size: 4090 1084 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3379 1353 Size: 2026 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4036 4410 4556 387 Size: 1064 138 2447 374 146 1280 Fragments arranged by size: 2447 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 3512 4109 4896 5164 1239 Size: 393 503 1377 597 787 268 1524 Fragments arranged by size: 1524 1377 787 597 503 393 268 EcoO109I rG'GnC_Cy Cuts at: 2276 2276 Size: 5449 EcoRI G'AATT_C Cuts at: 2591 2758 3136 2591 Size: 167 378 4904 Fragments arranged by size: 4904 378 167 FspI TGC'GCA Cuts at: 3955 3955 Size: 5449 HaeII r_GCGC'y Cuts at: 1678 4596 1678 Size: 2918 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2566 2598 4090 4122 4164 Size: 32 135 32 994 32 1492 32 42 Cuts at: 4164 4196 1373 Size: 32 2626 Fragments arranged by size: 2626 1492 994 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2331 2793 3039 669 Size: 988 281 393 462 246 3079 Fragments arranged by size: 3079 988 462 393 281 246 HindIII A'AGCT_T Cuts at: 748 2336 748 Size: 1588 3861 HpaI GTT'AAC Cuts at: 3039 3039 Size: 5449 KpnI G_GTAC'C Cuts at: 2774 2774 Size: 5449 MfeI C'AATT_G Cuts at: 3048 3048 Size: 5449 MluI A'CGCG_T Cuts at: 5325 5325 Size: 5449 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2341 2436 4563 4747 1305 Size: 1036 95 2127 184 2007 Fragments arranged by size: 2127 2007 1036 184 95 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3627 Fragments arranged by size: 3627 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2446 2615 2721 3407 3766 3925 Size: 648 873 407 169 106 686 359 159 Cuts at: 3925 518 Size: 2042 Fragments arranged by size: 2042 873 686 648 407 359 169 159 106 MspA1I CmG'CkG Cuts at: 1606 2865 3542 4690 4935 1606 Size: 1259 677 1148 245 2120 Fragments arranged by size: 2120 1259 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4897 NdeI CA'TA_TG Cuts at: 387 387 Size: 5449 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5449 NotI GC'GGCC_GC Cuts at: 2896 2896 Size: 5449 NspI r_CATG'y Cuts at: 1132 1229 2701 2807 4352 1132 Size: 97 1472 106 1545 2229 Fragments arranged by size: 2229 1545 1472 106 97 PasI CC'CwG_GG Cuts at: 1348 2651 1348 Size: 1303 4146 PciI A'CATG_T Cuts at: 4348 4348 Size: 5449 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5449 PmeI GTTT'AAAC Cuts at: 2753 2753 Size: 5449 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3518 3550 5031 5063 3518 Size: 32 1481 32 3904 Fragments arranged by size: 3904 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2276 2276 Size: 5449 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5449 PsiI TTA'TAA Cuts at: 3019 3019 Size: 5449 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2246 2278 2246 Size: 32 5417 PstI C_TGCA'G Cuts at: 830 2448 2801 830 Size: 1618 353 3478 Fragments arranged by size: 3478 1618 353 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5449 SalI G'TCGA_C Cuts at: 1936 2791 1936 Size: 855 4594 SbfI CC_TGCA'GG Cuts at: 2801 2801 Size: 5449 ScaI AGT'ACT Cuts at: 3697 5409 3697 Size: 1712 3737 SfcI C'TryA_G Cuts at: 826 1045 2444 2797 3932 4613 4804 826 Size: 219 1399 353 1135 681 191 1471 Fragments arranged by size: 1471 1399 1135 681 353 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5449 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5449 SmaI CCC'GGG Cuts at: 1872 2776 1872 Size: 904 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3553 4454 4716 820 Size: 197 926 1610 901 262 1553 Fragments arranged by size: 1610 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3240 493 Size: 2747 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5449 SphI G_CATG'C Cuts at: 1132 2807 1132 Size: 1675 3774 SspI AAT'ATT Cuts at: 5 52 3373 5 Size: 47 3321 2081 Fragments arranged by size: 3321 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5449 StyI C'CwwG_G Cuts at: 513 1065 2888 513 Size: 552 1823 3074 Fragments arranged by size: 3074 1823 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3476 3493 3646 3831 4276 325 Size: 1255 1896 17 153 185 445 1498 Fragments arranged by size: 1896 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2659 3695 5407 Size: 274 80 33 51 1443 682 1036 1712 Cuts at: 5407 96 Size: 138 Fragments arranged by size: 1712 1443 1036 682 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2416 2448 5409 5441 2416 Size: 32 2961 32 2424 Fragments arranged by size: 2961 2424 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5449 XbaI T'CTAG_A Cuts at: 2785 2785 Size: 5449 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2165 2545 2165 Size: 380 5069 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5449 XmaI C'CCGG_G Cuts at: 1870 2774 1870 Size: 904 4545 XmnI GAAnn'nnTTC Cuts at: 3578 3578 Size: 5449 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3257 276 Size: 53 83 186 936 1723 2468 Fragments arranged by size: 2468 1723 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI