(Linear) (Six-base) MAPSORT of: KIEE6231.seq Check: 6816 from: 1 to: 744 KIEE6231.orf With 182 enzymes: SgfI * March 16, 2015 11:28 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 744 Size: 130 614 AleI CACnn'nnGTG Cuts at: 0 441 610 744 Size: 441 169 134 Fragments arranged by size: 441 169 134 ApoI r'AATT_y Cuts at: 0 219 586 744 Size: 219 367 158 Fragments arranged by size: 367 219 158 BamHI G'GATC_C Cuts at: 0 550 744 Size: 550 194 BglI GCCn_nnn'nGGC Cuts at: 0 544 744 Size: 544 200 BglII A'GATC_T Cuts at: 0 155 744 Size: 155 589 BsaI GGTCTCn'nnnn_ Cuts at: 0 19 744 Size: 19 725 BsiHKAI G_wGCw'C Cuts at: 0 41 375 605 744 Size: 41 334 230 139 Fragments arranged by size: 334 230 139 41 BsmI GAATG_Cn' Cuts at: 0 362 744 Size: 362 382 Bsp1286I G_dGCh'C Cuts at: 0 41 375 605 744 Size: 41 334 230 139 Fragments arranged by size: 334 230 139 41 BspHI T'CATG_A Cuts at: 0 498 711 744 Size: 498 213 33 Fragments arranged by size: 498 213 33 BstXI CCAn_nnnn'nTGG Cuts at: 0 336 744 Size: 336 408 BstYI r'GATC_y Cuts at: 0 155 550 744 Size: 155 395 194 Fragments arranged by size: 395 194 155 BtgI C'CryG_G Cuts at: 0 515 744 Size: 515 229 BtsI GCAGTG_nn' Cuts at: 0 436 448 605 744 Size: 436 12 157 139 Fragments arranged by size: 436 157 139 12 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 130 744 Size: 130 614 EcoO109I rG'GnC_Cy Cuts at: 0 271 744 Size: 271 473 EcoRI G'AATT_C Cuts at: 0 586 744 Size: 586 158 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 561 593 744 Size: 561 32 151 Fragments arranged by size: 561 151 32 HincII GTy'rAC Cuts at: 0 326 744 Size: 326 418 HindIII A'AGCT_T Cuts at: 0 331 744 Size: 331 413 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 336 431 744 Size: 336 95 313 Fragments arranged by size: 336 313 95 MslI CAynn'nnrTG Cuts at: 0 34 441 610 716 744 Size: 34 407 169 106 28 Fragments arranged by size: 407 169 106 34 28 NspI r_CATG'y Cuts at: 0 696 744 Size: 696 48 PasI CC'CwG_GG Cuts at: 0 646 744 Size: 646 98 PpuMI rG'GwC_Cy Cuts at: 0 271 744 Size: 271 473 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 241 273 744 Size: 241 32 471 Fragments arranged by size: 471 241 32 PstI C_TGCA'G Cuts at: 0 443 744 Size: 443 301 SfcI C'TryA_G Cuts at: 0 439 744 Size: 439 305 TatI w'GTAC_w Cuts at: 0 654 744 Size: 654 90 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 411 443 744 Size: 411 32 301 Fragments arranged by size: 411 301 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 160 540 744 Size: 160 380 204 Fragments arranged by size: 380 204 160 Enzymes that do cut: AcuI AleI ApoI BamHI BglI BglII BsaI BsiHKAI BsmI Bsp1286I BspHI BstXI BstYI BtgI BtsI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MmeI MslI NspI PasI PpuMI PsrI PstI SfcI TatI TstI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI KasI KpnI MfeI MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI