################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI Bsp1407I BspEI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I CpoI DraI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HpaI HpaII KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 231 GACGTC GACGT^C AcyI 231 GACGTC G[GA]^CG[CT]C AlwI 601 GGATC GGATC(4/5) AlwNI 1018 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 1014 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 905 GAGCAC G[AT]GC[AT]^C BanII 961 GAGCCC G[GA]GC[CT]^C BfaI 890 CTAG C^TAG BsaHI 231 GACGTC G[GA]^CG[CT]C BsiHKAI 905 GAGCAC G[AT]GC[AT]^C BsmI 417 GCATTC GAATGC(1/-1) BspDI 1063 ATCGAT AT^CGAT BspMI 665 ACCTGC ACCTGC(4/8) BsrBI 999 CCGCTC CCGCTC(-3/-3) BsrDI 429 CATTGC GCAATG(2/0) BstAPI 907 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 48 TTCGAA TT^CGAA BstXI 622 CCAATGTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 549 GCGC GCG^C ClaI 1063 ATCGAT AT^CGAT DraII 892 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 892 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 597 CCATGG C^C[AT][AT]GG EcoT22I 255 ATGCAT ATGCA^T HhaI 549 GCGC GCG^C Hin1I 231 GACGTC G[GA]^CG[CT]C HinP1I 549 GCGC G^CGC HincII 1070 GTCGAC GT[CT]^[GA]AC HindII 1070 GTCGAC GT[CT]^[GA]AC HindIII 295 AAGCTT A^AGCTT MaeI 890 CTAG C^TAG MslI 645 CATCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 597 CCATGG C^CATGG NlaIV 32 GGCCCC GG[ACGT]^[ACGT]CC NsiI 255 ATGCAT ATGCA^T NspI 512 ACATGC [GA]CATG^[CT] NspV 48 TTCGAA TT^CGAA PaeR7I 564 CTCGAG C^TCGAG PleI 449 GACTC GAGTC(4/5) PshAI 227 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 1070 GTCGAC G^TCGAC SapI 610 GCTCTTC GCTCTTC(1/4) SexAI 954 ACCTGGT A^CC[AT]GGT SfuI 48 TTCGAA TT^CGAA SspI 485 AATATT AAT^ATT StyI 597 CCATGG C^C[AT][AT]GG Tsp45I 803 GTGAC ^GT[GC]AC XhoI 564 CTCGAG C^TCGAG XmnI 1014 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 864 GTCTAC GT^[AC][GT]AC 1070 GTCGAC AciI 999 CCGC CCGC(-3/-1) 4 GCGG AcsI 70 AAATTC [GA]^AATT[CT] 405 AAATTT AfaI 184 GTAC GT^AC 382 GTAC ApoI 70 AAATTC [GA]^AATT[CT] 405 AAATTT AvaI 463 CCCGAG C^[CT]CG[GA]G 564 CTCGAG BciVI 925 GGATAC GTATCC(6/5) 593 GTATCC BpmI 714 CTCCAG CTGGAG(16/14) 1042 CTCCAG BsaI 89 GAGACC GGTCTC(1/5) 831 GGTCTC BseRI 19 GAGGAG GAGGAG(10/8) 979 GAGGAG BsoBI 463 CCCGAG C^[CT]CG[GA]G 564 CTCGAG Cfr13I 32 GGCCC G^G[ACGT]CC 893 GGGCC EarI 611 CTCTTC CTCTTC(1/4) 1120 GAAGAG Ksp632I 611 CTCTTC CTCTTC(1/4) 1120 GAAGAG MaeII 137 ACGT A^CGT 232 ACGT MspA1I 520 CAGCTG C[AC]G^C[GT]G 605 CAGCTG PstI 222 CTGCAG CTGCA^G 517 CTGCAG PvuII 520 CAGCTG CAG^CTG 605 CAGCTG RsaI 184 GTAC GT^AC 382 GTAC Sau96I 32 GGCCC G^G[ACGT]CC 893 GGGCC SmlI 564 CTCGAG C^T[CT][GA]AG 1002 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AluI BbvI BmyI BsaJI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII DsaI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII Sau3AI ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I