Restriction map for
pFN21AE3928
(Circular) (Six-base) MAPSORT of: pFN21AE3928.seq Check: 487 from: 1 to: 5893
pFN21AE3928 5893 bp
With 182 enzymes: SgfI *
March 28, 2012 11:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2679 2679
Size: 5893
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2240 3703 278
Size: 53 83 186 936 704 1463 2468
Fragments arranged by size:
2468 1463 936 704 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2870 3076 3236 1937
Size: 933 206 160 4594
Fragments arranged by size:
4594 933 206 160
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2879 3120 3956 5340 5608 1632
Size: 1247 241 836 1384 268 1917
Fragments arranged by size:
1917 1384 1247 836 268 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2547 1184
Size: 1363 4530
AlwNI CAG_nnn'CTG
Cuts at: 3028 3636 5208 3028
Size: 608 1572 3713
Fragments arranged by size:
3713 1572 608
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 2075 2410 3202 3428 3580 5689 1493
Size: 582 335 792 226 152 2109 1697
Fragments arranged by size:
2109 1697 792 582 335 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 2468 2569 3218 1870
Size: 73 525 101 649 4545
Fragments arranged by size:
4545 649 525 101 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2970 3212 1391
Size: 1579 242 4072
Fragments arranged by size:
4072 1579 242
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2609 2924 3785 4995 2609
Size: 315 861 1210 3507
Fragments arranged by size:
3507 1210 861 315
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BfrBI ATG'CAT
Cuts at: 2262 2262
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4504 136
Size: 107 122 71 631 605 337 2495 1525
Fragments arranged by size:
2495 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5888 5888
Size: 5893
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 2692 3954 5110 1790
Size: 902 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 902
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2378 2648 3624
Size: 416 53 83 399 24 961 270 976
Cuts at: 3624 4582 442
Size: 958 1753
Fragments arranged by size:
1753 976 961 958 416 399 270 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2704 3032 4553 1239
Size: 1465 328 1521 2579
Fragments arranged by size:
2579 1521 1465 328
Bpu10I CC'TnA_GC
Cuts at: 2331 2331
Size: 5893
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2992 3174 4018 4883 5181 2992
Size: 182 844 865 298 3704
Fragments arranged by size:
3704 865 844 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2088 2842 4556 882
Size: 617 589 754 1714 2219
Fragments arranged by size:
2219 1714 754 617 589
BsaAI yAC'GTr
Cuts at: 493 1204 3684 493
Size: 711 2480 2702
Fragments arranged by size:
2702 2480 711
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2237 3700 4082 4717
Size: 53 83 186 936 704 1463 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1463 1451 936 704 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5668
Fragments arranged by size:
5668 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2999 1186
Size: 853 960 4080
Fragments arranged by size:
4080 960 853
BseYI C'CCAG_C
Cuts at: 5096 5592 5096
Size: 496 5397
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5320
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2914 3954 4039 5110 2914
Size: 1040 85 1071 3697
Fragments arranged by size:
3697 1071 1040 85
BsmI GAATG_Cn'
Cuts at: 2422 3404 3497 2422
Size: 982 93 4818
Fragments arranged by size:
4818 982 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2692 2914 2970 3212 3954 4039 5110
Size: 399 902 222 56 242 742 85 1071
Cuts at: 5110 1391
Size: 2174
Fragments arranged by size:
2174 1071 902 742 399 242 222 85
56
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3782 5512 3782
Size: 1730 4163
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2679 3248 844
Size: 1835 569 3489
Fragments arranged by size:
3489 1835 569
BsrBI CCG'CTC
Cuts at: 1394 1823 3006 3780 1394
Size: 429 1183 774 3507
Fragments arranged by size:
3507 1183 774 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2433 4388 4562 65
Size: 1183 1185 1955 174 1396
Fragments arranged by size:
1955 1396 1185 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5893
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2918 794
Size: 2124 3769
BstBI TT'CG_AA
Cuts at: 2054 4697 2054
Size: 2643 3250
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstXI CCAn_nnnn'nTGG
Cuts at: 2634 2634
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 3223 3590 3975 3992 5444 5530 5542 5888
Size: 1251 367 385 17 1452 86 12 346
Cuts at: 5888 1972
Size: 1977
Fragments arranged by size:
1977 1452 1251 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 2502 2602 513
Size: 552 939 14 484 100 3804
Fragments arranged by size:
3804 939 552 484 100 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 4203 4223 3409
Size: 794 20 5079
Fragments arranged by size:
5079 794 20
ClaI AT'CG_AT
Cuts at: 1554 3069 1554
Size: 1515 4378
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2814 2849 529
Size: 35 2250 35 3573
Fragments arranged by size:
3573 2250 35 35
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4900 809
Size: 507 3584 1802
Fragments arranged by size:
3584 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 3120 3823 1353
Size: 1269 498 703 3423
Fragments arranged by size:
3423 1269 703 498
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2704 2879 3032 3120 3956 4553 5340
Size: 393 1072 175 153 88 836 597 787
Cuts at: 5340 5608 1239
Size: 268 1524
Fragments arranged by size:
1524 1072 836 787 597 393 268 175
153 88
EcoO109I rG'GnC_Cy
Cuts at: 2898 2898
Size: 5893
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2464 2496 2822 2854 4534
Size: 32 135 32 892 32 326 32 1680
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1680 892 326 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3077 3237 3483 669
Size: 988 281 1139 160 246 3079
Fragments arranged by size:
3079 1139 988 281 246 160
HindIII A'AGCT_T
Cuts at: 748 2300 748
Size: 1552 4341
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2149 5007 5191 1305
Size: 844 2858 184 2007
Fragments arranged by size:
2858 2007 844 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3851 4210 4369 518
Size: 648 1488 1197 359 159 2042
Fragments arranged by size:
2042 1488 1197 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2612 3309 3986 5134 5379 1606
Size: 921 85 697 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 697 677 245 85
NcoI C'CATG_G
Cuts at: 513 1065 2004 2602 513
Size: 552 939 598 3804
Fragments arranged by size:
3804 939 598 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5893
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NsiI A_TGCA'T
Cuts at: 2264 2264
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 2521 3251 4796 1132
Size: 97 1292 730 1545 2229
Fragments arranged by size:
2229 1545 1292 730 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5893
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5893
PfoI T'CCnGG_A
Cuts at: 3027 3027
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2236 1613
Size: 623 5270
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2231 2526 3245 830
Size: 1401 295 719 3478
Fragments arranged by size:
3478 1401 719 295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
PvuII CAG'CTG
Cuts at: 2527 2612 2527
Size: 85 5808
SalI G'TCGA_C
Cuts at: 1936 3075 3235 1936
Size: 1139 160 4594
Fragments arranged by size:
4594 1139 160
SapI GCTCTTCn'nnn_
Cuts at: 2622 2622
Size: 5893
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SexAI A'CCwGG_T
Cuts at: 2959 2959
Size: 5893
SfcI C'TryA_G
Cuts at: 826 1045 2108 2227 2522 2871 2935 3241 4376
Size: 219 1063 119 295 349 64 306 1135
Cuts at: 4376 5057 5248 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1063 681 349 306 295 219
191 119 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2569 3007 3189 3997 4898 5160
Size: 197 926 626 438 182 808 901 262
Cuts at: 5160 820
Size: 1553
Fragments arranged by size:
1553 926 901 808 626 438 262 197
182
SnaBI TAC'GTA
Cuts at: 493 3684 493
Size: 3191 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 2492 3817 5
Size: 47 2440 1325 2081
Fragments arranged by size:
2440 2081 1325 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5893
StyI C'CwwG_G
Cuts at: 513 1065 2004 2602 3332 513
Size: 552 939 598 730 3074
Fragments arranged by size:
3074 939 730 598 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 3920 3937 4090 4275 4720 325
Size: 1255 817 1523 17 153 185 445 1498
Fragments arranged by size:
1523 1498 1255 817 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 4139 5851
Size: 274 80 33 51 1443 211 1951 1712
Cuts at: 5851 96
Size: 138
Fragments arranged by size:
1951 1712 1443 274 211 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XhoI C'TCGA_G
Cuts at: 1943 2569 1943
Size: 626 5267
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 3023 4022 3023
Size: 999 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2238 3701 276
Size: 53 83 186 936 704 1463 2468
Fragments arranged by size:
2468 1463 936 704 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI