################################## # NO CUT ENZYMES ( 210 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AflII AgeI AhdI AluI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeIII HapII HgaI Hin1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AfaI 188 GTAC GT^AC AflIII 164 ACATGT A^C[GA][CT]GT AlwI 63 GATCC GGATC(4/5) AvaII 244 GGACC G^G[AT]CC BsmFI 131 GTCCC GGGAC(10/14) BspLU11I 164 ACATGT A^CATGT BstBI 236 TTCGAA TT^CGAA BstF5I 127 GGATG GGATG(2/0) Cfr13I 244 GGACC G^G[ACGT]CC DdeI 110 CTCAG C^T[ACGT]AG DpnII 63 GATC ^GATC FokI 127 GGATG GGATG(9/13) MboI 63 GATC ^GATC NdeII 63 GATC ^GATC NlaIV 244 GGACCC GG[ACGT]^[ACGT]CC NspI 164 ACATGT [GA]CATG^[CT] NspV 236 TTCGAA TT^CGAA RsaI 188 GTAC GT^AC Sau3AI 63 GATC ^GATC Sau96I 244 GGACC G^G[ACGT]CC SfuI 236 TTCGAA TT^CGAA SspI 223 AATATT AAT^ATT TaqI 237 TCGA T^CGA Tsp45I 161 GTGAC ^GT[GC]AC Tsp509I 71 AATT ^AATT TspRI 159 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 237 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### BsiYI 67 CCTCAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 148 CCTGTGTGTGG BslI 67 CCTCAATTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 148 CCTGTGTGTGG BssKI 36 CCTGG ^CC[ACGT]GG 124 CCAGG BstNI 36 CCTGG CC^[AT]GG 124 CCAGG EcoRII 36 CCTGG ^CC[AT]GG 124 CCAGG HaeII 3 GGCGCT [GA]GCGC^[CT] 24 GGCGCT MaeIII 43 GTTAC ^GT[ACGT]AC 161 GTGAC MnlI 56 CCTC CCTC(7/6) 67 CCTC MseI 143 TTAA T^TAA 227 TTAA MvaI 36 CCTGG CC^[AT]GG 124 CCAGG MwoI 6 GCTGTCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 18 GCGCTTGGCGC NlaIII 165 CATG CATG^ 198 CATG ScrFI 36 CCTGG CC^[ACGT]GG 124 CCAGG Tru9I 143 TTAA T^TAA 227 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BbvI CfoI Fnu4HI HhaI HinP1I ItaI TseI