(Circular) (Six-base) MAPSORT of: pFN21AE5124.seq Check: 6771 from: 1 to: 4957 pFN21AE5124 4957 bp With 182 enzymes: SgfI * January 30, 2014 17:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2767 278 Size: 53 83 186 936 1231 2468 Fragments arranged by size: 2468 1231 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2300 1937 Size: 363 4594 Acc65I G'GTAC_C Cuts at: 2278 2278 Size: 4957 AclI AA'CG_TT Cuts at: 1563 3084 3457 1563 Size: 1521 373 3063 Fragments arranged by size: 3063 1521 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3020 4404 4672 1632 Size: 1388 1384 268 1917 Fragments arranged by size: 1917 1388 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4760 AflIII A'CryG_T Cuts at: 1203 2169 3856 4833 1203 Size: 966 1687 977 1327 Fragments arranged by size: 1687 1327 977 966 AgeI A'CCGG_T Cuts at: 3788 3788 Size: 4957 AhdI GACnn_n'nnGTC Cuts at: 3686 3686 Size: 4957 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4957 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4957 AlwNI CAG_nnn'CTG Cuts at: 2700 4272 2700 Size: 1572 3385 ApaLI G'TGCA_C Cuts at: 3014 4170 3014 Size: 1156 3801 ApoI r'AATT_y Cuts at: 1493 2266 2492 2644 4753 1493 Size: 773 226 152 2109 1697 Fragments arranged by size: 2109 1697 773 226 152 AseI AT'TA_AT Cuts at: 160 1032 3511 160 Size: 872 2479 1606 Fragments arranged by size: 2479 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4957 AvaI C'yCGr_G Cuts at: 1870 1943 2282 1870 Size: 73 339 4545 Fragments arranged by size: 4545 339 73 BamHI G'GATC_C Cuts at: 2287 2654 2287 Size: 367 4590 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2278 3733 618 Size: 325 217 627 491 1455 1842 Fragments arranged by size: 1842 1455 627 491 325 217 BanII G_rGCy'C Cuts at: 1391 2276 1391 Size: 885 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3986 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3148 3182 1903 Size: 34 1211 34 3678 Fragments arranged by size: 3678 1211 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2849 4059 2849 Size: 1210 3747 BclI T'GATC_A Cuts at: 1548 1548 Size: 4957 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3568 136 Size: 107 122 71 631 605 1896 1525 Fragments arranged by size: 1896 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4952 4952 Size: 4957 BlpI GC'TnA_GC Cuts at: 2374 2374 Size: 4957 Bme1580I G_kGCm'C Cuts at: 1790 3018 4174 1790 Size: 1228 1156 2573 Fragments arranged by size: 2573 1228 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4957 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2688 3646 442 Size: 416 53 83 399 24 1271 958 1753 Fragments arranged by size: 1753 1271 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4957 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3617 1239 Size: 2378 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3082 3947 4245 3082 Size: 865 298 3794 Fragments arranged by size: 3794 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3620 882 Size: 617 2121 2219 Fragments arranged by size: 2219 2121 617 BsaAI yAC'GTr Cuts at: 493 1204 2748 493 Size: 711 1544 2702 Fragments arranged by size: 2702 1544 711 BsaBI GATnn'nnATC Cuts at: 4951 4951 Size: 4957 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2764 3146 3781 275 Size: 53 83 186 936 1231 382 635 1451 Fragments arranged by size: 1451 1231 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3390 3788 4062 4209 4620 3390 Size: 398 274 147 411 3727 Fragments arranged by size: 3727 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4732 Fragments arranged by size: 4732 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4957 BseYI C'CCAG_C Cuts at: 4160 4656 4160 Size: 496 4461 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4384 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2407 3168 4196 1540 Size: 275 186 406 761 1028 2301 Fragments arranged by size: 2301 1028 761 406 275 186 BsiHKAI G_wGCw'C Cuts at: 3018 3103 4174 3018 Size: 85 1071 3801 Fragments arranged by size: 3801 1071 85 BsmI GAATG_Cn' Cuts at: 2468 2561 2468 Size: 93 4864 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2276 3018 3103 4174 1391 Size: 399 486 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 486 399 85 BspEI T'CCGG_A Cuts at: 4620 4620 Size: 4957 BspHI T'CATG_A Cuts at: 2846 4576 2846 Size: 1730 3227 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2312 844 Size: 1468 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2844 1394 Size: 429 1021 3507 Fragments arranged by size: 3507 1021 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3452 3626 65 Size: 1183 2204 174 1396 Fragments arranged by size: 2204 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3601 3788 1700 Size: 110 1791 187 2869 Fragments arranged by size: 2869 1791 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4957 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4957 BssSI C'ACGA_G Cuts at: 3017 4029 3017 Size: 1012 3945 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4957 BstBI TT'CG_AA Cuts at: 2242 3761 2242 Size: 1519 3438 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4957 BstYI r'GATC_y Cuts at: 1972 2287 2654 3039 3056 4508 4594 4606 4952 Size: 315 367 385 17 1452 86 12 346 Cuts at: 4952 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 315 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3466 Fragments arranged by size: 3466 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4218 Fragments arranged by size: 4218 683 56 BtsI GCAGTG_nn' Cuts at: 2170 2473 3267 3287 2170 Size: 303 794 20 3840 Fragments arranged by size: 3840 794 303 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4957 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4922 DraI TTT'AAA Cuts at: 2261 2261 Size: 4957 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3964 809 Size: 507 2648 1802 Fragments arranged by size: 2648 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2404 3293 4922 Size: 54 1425 50 275 18 574 889 1629 Cuts at: 4922 8 Size: 43 Fragments arranged by size: 1629 1425 889 574 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2404 1537 Size: 275 592 4090 Fragments arranged by size: 4090 592 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2887 1353 Size: 1534 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3544 3918 4064 387 Size: 1064 138 1955 374 146 1280 Fragments arranged by size: 1955 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3020 3617 4404 4672 1239 Size: 393 1388 597 787 268 1524 Fragments arranged by size: 1524 1388 787 597 393 268 EcoRI G'AATT_C Cuts at: 2266 2644 2266 Size: 378 4579 FspI TGC'GCA Cuts at: 3463 3463 Size: 4957 HaeII r_GCGC'y Cuts at: 1678 2012 2033 4104 1678 Size: 334 21 2071 2531 Fragments arranged by size: 2531 2071 334 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3598 3630 3672 3704 1373 Size: 32 135 32 2026 32 42 32 2626 Fragments arranged by size: 2626 2026 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2301 2547 669 Size: 988 281 363 246 3079 Fragments arranged by size: 3079 988 363 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4957 HpaI GTT'AAC Cuts at: 2547 2547 Size: 4957 KpnI G_GTAC'C Cuts at: 2282 2282 Size: 4957 MfeI C'AATT_G Cuts at: 2556 2556 Size: 4957 MluI A'CGCG_T Cuts at: 4833 4833 Size: 4957 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4071 4255 1305 Size: 2766 184 2007 Fragments arranged by size: 2766 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3135 Fragments arranged by size: 3135 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2915 3274 3433 518 Size: 648 1749 359 159 2042 Fragments arranged by size: 2042 1749 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2373 3050 4198 4443 1606 Size: 767 677 1148 245 2120 Fragments arranged by size: 2120 1148 767 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3466 Fragments arranged by size: 3466 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4957 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4957 NotI GC'GGCC_GC Cuts at: 2404 2404 Size: 4957 NspI r_CATG'y Cuts at: 1132 1229 2173 2315 3860 1132 Size: 97 944 142 1545 2229 Fragments arranged by size: 2229 1545 944 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4957 PciI A'CATG_T Cuts at: 2169 3856 2169 Size: 1687 3270 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4957 PmeI GTTT'AAAC Cuts at: 2261 2261 Size: 4957 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3026 3058 4539 4571 3026 Size: 32 1481 32 3412 Fragments arranged by size: 3412 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4957 PsiI TTA'TAA Cuts at: 2527 2527 Size: 4957 PstI C_TGCA'G Cuts at: 830 2309 830 Size: 1479 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4957 SalI G'TCGA_C Cuts at: 1936 2299 1936 Size: 363 4594 SbfI CC_TGCA'GG Cuts at: 2309 2309 Size: 4957 ScaI AGT'ACT Cuts at: 3205 4917 3205 Size: 1712 3245 SfcI C'TryA_G Cuts at: 826 1045 2305 3440 4121 4312 826 Size: 219 1260 1135 681 191 1471 Fragments arranged by size: 1471 1260 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4957 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4957 SmaI CCC'GGG Cuts at: 1872 2284 1872 Size: 412 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3061 3962 4224 820 Size: 197 926 1118 901 262 1553 Fragments arranged by size: 1553 1118 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2748 493 Size: 2255 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4957 SphI G_CATG'C Cuts at: 1132 2315 1132 Size: 1183 3774 SspI AAT'ATT Cuts at: 5 52 2230 2881 5 Size: 47 2178 651 2081 Fragments arranged by size: 2178 2081 651 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4957 StyI C'CwwG_G Cuts at: 513 1065 2004 2396 513 Size: 552 939 392 3074 Fragments arranged by size: 3074 939 552 392 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2984 3001 3154 3339 3784 325 Size: 1255 1404 17 153 185 445 1498 Fragments arranged by size: 1498 1404 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2192 3203 4915 Size: 274 80 33 51 1443 215 1011 1712 Cuts at: 4915 96 Size: 138 Fragments arranged by size: 1712 1443 1011 274 215 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4917 4949 4917 Size: 32 4925 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4957 XbaI T'CTAG_A Cuts at: 2293 2293 Size: 4957 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4957 XmaI C'CCGG_G Cuts at: 1870 2282 1870 Size: 412 4545 XmnI GAAnn'nnTTC Cuts at: 3086 3086 Size: 4957 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2765 276 Size: 53 83 186 936 1231 2468 Fragments arranged by size: 2468 1231 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI