################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflIII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AvaI AviII BamHI BanI BbeI BbrPI BbvCI BcgI BcnI BglI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstUI Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI EclXI Eco47III Eco52I EcoO109I EcoRI EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI TaqI Tth111I TthHB8I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 1591 TCCGGA T^CCGGA AciI 1472 GCGG CCGC(-3/-1) AflII 1750 CTTAAG C^TTAAG AhdI 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1601 CCTCAGG CC^T[ACGT]AGG AspEI 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 703 GGACC G^G[AT]CC AvrII 1698 CCTAGG C^CTAGG BalI 2113 TGGCCA TGG^CCA BciVI 1714 GGATAC GTATCC(6/5) BfrI 1750 CTTAAG C^TTAAG BlnI 1698 CCTAGG C^CTAGG BsaWI 1591 TCCGGA [AT]^CCGG[AT] BseAI 1591 TCCGGA T^CCGGA Bsp1407I 1078 TGTACA T^GTACA BspEI 1591 TCCGGA T^CCGGA BspHI 1401 TCATGA T^CATGA BsrGI 1078 TGTACA T^GTACA Bst1107I 1060 GTATAC GTA^TAC BstEII 1303 GGTTACC G^GT[ACGT]ACC BstPI 1303 GGTTACC G^GT[ACGT]ACC BstXI 164 CCAAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1060 GTATAC GTA^TAC Bsu36I 1601 CCTCAGG CC^T[ACGT]AGG EaeI 2113 TGGCCA [CT]^GGCC[GA] Eam1105I 1361 GACTAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1601 CCTCAGG CC^T[ACGT]AGG EcoNI 1523 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1303 GGTTACC G^GT[ACGT]ACC EcoRV 223 GATATC GAT^ATC HapII 1592 CCGG C^CGG HincII 751 GTTGAC GT[CT]^[GA]AC HindII 751 GTTGAC GT[CT]^[GA]AC HpaII 1592 CCGG C^CGG MaeII 1120 ACGT A^CGT MluNI 2113 TGGCCA TGG^CCA MroI 1591 TCCGGA T^CCGGA MscI 2113 TGGCCA TGG^CCA MslI 1563 CACTTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 863 CAGCTG C[AC]G^C[GT]G MspI 1592 CCGG C^CGG MwoI 1501 GCTTGTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 1490 CATATG CA^TATG PleI 469 GAGTC GAGTC(4/5) PvuII 863 CAGCTG CAG^CTG RcaI 1401 TCATGA T^CATGA SacI 1409 GAGCTC GAGCT^C ScaI 1977 AGTACT AGT^ACT SspBI 1078 TGTACA T^GTACA StuI 64 AGGCCT AGG^CCT Tsp45I 1117 GTGAC ^GT[GC]AC XcmI 608 CCATTCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 1060 GTATAC GT^[AC][GT]AC 2052 GTCTAC Asp700I 62 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1242 GAAAAAATTC AspHI 192 GAGCAC G[AT]GC[AT]^C 1409 GAGCTC BanII 1186 GGGCTC G[GA]GC[CT]^C 1409 GAGCTC BclI 960 TGATCA T^GATCA 2088 TGATCA BpmI 1164 CTCCAG CTGGAG(16/14) 1811 CTCCAG BsgI 1560 CTGCAC GTGCAG(16/14) 682 GTGCAG BsiHKAI 192 GAGCAC G[AT]GC[AT]^C 1409 GAGCTC BsmFI 1929 GGGAC GGGAC(10/14) 1640 GTCCC BstYI 798 AGATCC [GA]^GATC[CT] 901 AGATCC EarI 549 CTCTTC CTCTTC(1/4) 189 GAAGAG FbaI 960 TGATCA T^GATCA 2088 TGATCA Ksp632I 549 CTCTTC CTCTTC(1/4) 189 GAAGAG MflI 798 AGATCC [GA]^GATC[CT] 901 AGATCC SapI 189 GAAGAGC GCTCTTC(1/4) 548 GCTCTTC SfaNI 1436 GATGC GCATC(5/9) 449 GCATC SmlI 1431 CTCAAG C^T[CT][GA]AG 1750 CTTAAG SspI 1218 AATATT AAT^ATT 1543 AATATT XhoII 798 AGATCC [GA]^GATC[CT] 901 AGATCC XmnI 62 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1242 GAAAAAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI StyI TfiI Tru9I TseI Tsp509I TspRI Van91I