(Circular) (Six-base) MAPSORT of: pFN21AE6727.seq Check: 4185 from: 1 to: 6841 pFN21AE6727 6841 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4651 278 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3066 4058 4184 1937 Size: 1129 992 126 4594 Fragments arranged by size: 4594 1129 992 126 Acc65I G'GTAC_C Cuts at: 4162 4162 Size: 6841 AclI AA'CG_TT Cuts at: 1563 4968 5341 1563 Size: 3405 373 3063 Fragments arranged by size: 3405 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 2213 3062 3901 4904 6288 6556 1632 Size: 402 179 849 839 1003 1384 268 1917 Fragments arranged by size: 1917 1384 1003 849 839 402 268 179 AflII C'TTAA_G Cuts at: 820 1017 3755 820 Size: 197 2738 3906 Fragments arranged by size: 3906 2738 197 AflIII A'CryG_T Cuts at: 1203 5740 6717 1203 Size: 4537 977 1327 Fragments arranged by size: 4537 1327 977 AgeI A'CCGG_T Cuts at: 5672 5672 Size: 6841 AhdI GACnn_n'nnGTC Cuts at: 3371 5570 3371 Size: 2199 4642 AleI CACnn'nnGTG Cuts at: 1166 3572 1166 Size: 2406 4435 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2712 1184 Size: 1528 5313 AlwNI CAG_nnn'CTG Cuts at: 4584 6156 4584 Size: 1572 5269 ApaLI G'TGCA_C Cuts at: 4898 6054 4898 Size: 1156 5685 ApoI r'AATT_y Cuts at: 1493 3251 3349 3601 4150 4376 4528 6637 1493 Size: 1758 98 252 549 226 152 2109 1697 Fragments arranged by size: 2109 1758 1697 549 252 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 5395 160 Size: 872 4363 1606 Fragments arranged by size: 4363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 AvaI C'yCGr_G Cuts at: 1870 1943 4166 1870 Size: 73 2223 4545 Fragments arranged by size: 4545 2223 73 AvrII C'CTAG_G Cuts at: 3703 3703 Size: 6841 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3483 3516 3483 Size: 33 6808 BamHI G'GATC_C Cuts at: 4171 4538 4171 Size: 367 6474 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4162 5617 618 Size: 325 217 627 2375 1455 1842 Fragments arranged by size: 2375 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3195 3418 4160 1391 Size: 1804 223 742 4072 Fragments arranged by size: 4072 1804 742 223 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2664 2852 3907 928 Size: 971 765 188 1055 3862 Fragments arranged by size: 3862 1055 971 765 188 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5032 5066 1903 Size: 34 3095 34 3678 Fragments arranged by size: 3678 3095 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3713 4733 5943 3713 Size: 1020 1210 4611 Fragments arranged by size: 4611 1210 1020 BclI T'GATC_A Cuts at: 1548 2965 4093 1548 Size: 1417 1128 4296 Fragments arranged by size: 4296 1417 1128 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5452 136 Size: 107 122 71 631 605 3780 1525 Fragments arranged by size: 3780 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6836 6836 Size: 6841 BlpI GC'TnA_GC Cuts at: 4258 4258 Size: 6841 Bme1580I G_kGCm'C Cuts at: 1790 3956 4902 6058 1790 Size: 2166 946 1156 2573 Fragments arranged by size: 2573 2166 1156 946 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6841 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2803 3056 3347 Size: 416 53 83 399 24 1386 253 291 Cuts at: 3347 3959 4572 5530 442 Size: 612 613 958 1753 Fragments arranged by size: 1753 1386 958 613 612 416 399 291 253 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6841 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2445 2445 Size: 6841 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3154 3801 5501 1239 Size: 1915 647 1700 2579 Fragments arranged by size: 2579 1915 1700 647 Bpu10I CC'TnA_GC Cuts at: 3971 3971 Size: 6841 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3421 4966 5831 6129 3421 Size: 1545 865 298 4133 Fragments arranged by size: 4133 1545 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5504 882 Size: 617 4005 2219 Fragments arranged by size: 4005 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4632 493 Size: 711 3428 2702 Fragments arranged by size: 3428 2702 711 BsaBI GATnn'nnATC Cuts at: 6835 6835 Size: 6841 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4648 5030 5665 275 Size: 53 83 186 936 3115 382 635 1451 Fragments arranged by size: 3115 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3596 5274 5672 5946 6093 6504 3596 Size: 1678 398 274 147 411 3933 Fragments arranged by size: 3933 1678 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6616 Fragments arranged by size: 6616 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6841 BseYI C'CCAG_C Cuts at: 3811 6044 6540 3811 Size: 2233 496 4112 Fragments arranged by size: 4112 2233 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2708 3550 1163 Size: 573 972 842 4454 Fragments arranged by size: 4454 972 842 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4291 5052 6080 1540 Size: 275 186 2290 761 1028 2301 Fragments arranged by size: 2301 2290 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2201 3418 4902 4987 6058 2201 Size: 1217 1484 85 1071 2984 Fragments arranged by size: 2984 1484 1217 1071 85 BsmI GAATG_Cn' Cuts at: 4352 4445 4352 Size: 93 6748 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2201 3195 3418 3956 4160 4902 4987 Size: 399 411 994 223 538 204 742 85 Cuts at: 4987 6058 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 994 742 538 411 399 223 204 85 BspEI T'CCGG_A Cuts at: 3596 6504 3596 Size: 2908 3933 BspHI T'CATG_A Cuts at: 3406 4730 6460 3406 Size: 1324 1730 3787 Fragments arranged by size: 3787 1730 1324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4196 844 Size: 3352 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4728 1394 Size: 429 2905 3507 Fragments arranged by size: 3507 2905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 2628 3975 5336 5510 65 Size: 1183 1143 237 1347 1361 174 1396 Fragments arranged by size: 1396 1361 1347 1183 1143 237 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5485 5672 1700 Size: 110 3675 187 2869 Fragments arranged by size: 3675 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3083 96 Size: 2987 3854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6841 BssSI C'ACGA_G Cuts at: 4901 5913 4901 Size: 1012 5829 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6841 BstBI TT'CG_AA Cuts at: 5645 5645 Size: 6841 BstEII G'GTnAC_C Cuts at: 1184 3308 1184 Size: 2124 4717 BstXI CCAn_nnnn'nTGG Cuts at: 2176 2176 Size: 6841 BstYI r'GATC_y Cuts at: 1972 2803 2906 4171 4538 4923 4940 6392 6478 Size: 831 103 1265 367 385 17 1452 86 Cuts at: 6478 6490 6836 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1265 831 385 367 346 103 86 17 12 BstZ17I GTA'TAC Cuts at: 3067 3067 Size: 6841 Bsu36I CC'TnA_GG Cuts at: 3607 3607 Size: 6841 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5350 Fragments arranged by size: 5350 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6102 Fragments arranged by size: 6102 683 56 BtsI GCAGTG_nn' Cuts at: 2372 3216 4357 5151 5171 2372 Size: 844 1141 794 20 4042 Fragments arranged by size: 4042 1141 844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6841 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3520 3555 529 Size: 35 2956 35 3815 Fragments arranged by size: 3815 2956 35 35 DraI TTT'AAA Cuts at: 2080 3868 3996 4145 2080 Size: 1788 128 149 4776 Fragments arranged by size: 4776 1788 149 128 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5848 809 Size: 507 4532 1802 Fragments arranged by size: 4532 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4118 4288 5177 Size: 54 1425 50 275 18 2288 170 889 Cuts at: 5177 6806 8 Size: 1629 43 Fragments arranged by size: 2288 1629 1425 889 275 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4288 1537 Size: 275 2476 4090 Fragments arranged by size: 4090 2476 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2189 2560 4771 1353 Size: 836 371 2211 3423 Fragments arranged by size: 3423 2211 836 371 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5428 5802 5948 387 Size: 1064 138 3839 374 146 1280 Fragments arranged by size: 3839 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3416 3416 Size: 6841 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2213 3062 3154 3801 3901 4904 Size: 393 402 179 849 92 647 100 1003 Cuts at: 4904 5501 6288 6556 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1003 849 787 647 597 402 393 268 179 100 92 EcoNI CCTnn'n_nnAGG Cuts at: 3532 3532 Size: 6841 EcoRI G'AATT_C Cuts at: 4150 4528 4150 Size: 378 6463 EcoRV GAT'ATC Cuts at: 2230 2230 Size: 6841 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2091 2155 2091 Size: 64 6777 FspI TGC'GCA Cuts at: 5347 5347 Size: 6841 HaeII r_GCGC'y Cuts at: 1678 5988 1678 Size: 4310 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2537 2569 3285 Size: 32 135 32 428 32 505 32 716 Cuts at: 3285 3317 3357 3389 3419 3451 5482 5514 5556 Size: 32 40 32 30 32 2031 32 42 Cuts at: 5556 5588 1373 Size: 32 2626 Fragments arranged by size: 2626 2031 716 505 428 135 42 40 32 32 32 32 32 32 32 32 32 30 HincII GTy'rAC Cuts at: 669 1657 1938 2758 4185 4431 669 Size: 988 281 820 1427 246 3079 Fragments arranged by size: 3079 1427 988 820 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6841 HpaI GTT'AAC Cuts at: 4431 4431 Size: 6841 KpnI G_GTAC'C Cuts at: 4166 4166 Size: 6841 MfeI C'AATT_G Cuts at: 4440 4440 Size: 6841 MluI A'CGCG_T Cuts at: 6717 6717 Size: 6841 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 2347 3555 5955 6139 1305 Size: 739 303 1208 2400 184 2007 Fragments arranged by size: 2400 2007 1208 739 303 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4120 10 Size: 54 1425 343 2288 2731 Fragments arranged by size: 2731 2288 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3572 4799 5158 5317 518 Size: 648 2406 1227 359 159 2042 Fragments arranged by size: 2406 2042 1227 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2870 4257 4934 6082 6327 1606 Size: 1264 1387 677 1148 245 2120 Fragments arranged by size: 2120 1387 1264 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5350 Fragments arranged by size: 5350 939 552 NdeI CA'TA_TG Cuts at: 387 3496 387 Size: 3109 3732 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6841 NotI GC'GGCC_GC Cuts at: 4288 4288 Size: 6841 NspI r_CATG'y Cuts at: 1132 1229 4199 5744 1132 Size: 97 2970 1545 2229 Fragments arranged by size: 2970 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2989 1348 Size: 1641 5200 PciI A'CATG_T Cuts at: 5740 5740 Size: 6841 PflMI CCAn_nnn'nTGG Cuts at: 1108 2120 2717 4116 1108 Size: 1012 597 1399 3833 Fragments arranged by size: 3833 1399 1012 597 PmeI GTTT'AAAC Cuts at: 4145 4145 Size: 6841 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3402 3434 4910 4942 6423 6455 3402 Size: 32 1476 32 1481 32 3788 Fragments arranged by size: 3788 1481 1476 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6841 PsiI TTA'TAA Cuts at: 2291 4031 4411 2291 Size: 1740 380 4721 Fragments arranged by size: 4721 1740 380 PstI C_TGCA'G Cuts at: 830 4193 830 Size: 3363 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6841 PvuII CAG'CTG Cuts at: 2870 2870 Size: 6841 SacI G_AGCT'C Cuts at: 3418 3418 Size: 6841 SalI G'TCGA_C Cuts at: 1936 4183 1936 Size: 2247 4594 SapI GCTCTTCn'nnn_ Cuts at: 2189 2560 2189 Size: 371 6470 SbfI CC_TGCA'GG Cuts at: 4193 4193 Size: 6841 ScaI AGT'ACT Cuts at: 3984 5089 6801 3984 Size: 1105 1712 4024 Fragments arranged by size: 4024 1712 1105 SfcI C'TryA_G Cuts at: 826 1045 4189 5324 6005 6196 826 Size: 219 3144 1135 681 191 1471 Fragments arranged by size: 3144 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6841 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6841 SmaI CCC'GGG Cuts at: 1872 4168 1872 Size: 2296 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3436 3755 4945 5846 6108 820 Size: 197 926 1493 319 1190 901 262 1553 Fragments arranged by size: 1553 1493 1190 926 901 319 262 197 SnaBI TAC'GTA Cuts at: 493 4632 493 Size: 4139 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6841 SphI G_CATG'C Cuts at: 1132 4199 1132 Size: 3067 3774 SspI AAT'ATT Cuts at: 5 52 3225 3550 4765 5 Size: 47 3173 325 1215 2081 Fragments arranged by size: 3173 2081 1215 325 47 StuI AGG'CCT Cuts at: 1517 2071 1517 Size: 554 6287 StyI C'CwwG_G Cuts at: 513 1065 2004 3217 3382 3703 3768 4280 513 Size: 552 939 1213 165 321 65 512 3074 Fragments arranged by size: 3074 1213 939 552 512 321 165 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2670 4868 4885 5038 5223 5668 325 Size: 1255 1090 2198 17 153 185 445 1498 Fragments arranged by size: 2198 1498 1255 1090 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2409 3083 3138 Size: 274 80 33 51 1443 432 674 55 Cuts at: 3138 3982 5087 6799 96 Size: 844 1105 1712 138 Fragments arranged by size: 1712 1443 1105 844 674 432 274 138 80 55 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3458 3490 3927 3959 6801 6833 3458 Size: 32 437 32 2842 32 3466 Fragments arranged by size: 3466 2842 437 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6841 XbaI T'CTAG_A Cuts at: 4177 4177 Size: 6841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2620 2620 Size: 6841 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6841 XmaI C'CCGG_G Cuts at: 1870 4166 1870 Size: 2296 4545 XmnI GAAnn'nnTTC Cuts at: 2071 3251 4970 2071 Size: 1180 1719 3942 Fragments arranged by size: 3942 1719 1180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4649 276 Size: 53 83 186 936 3115 2468 Fragments arranged by size: 3115 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI BfrBI BsiWI BsmBI DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI