(Linear) (Six-base) MAPSORT of: KIEE6727.seq Check: 9371 from: 1 to: 2136 KIEE6727.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 1061 2053 2136 Size: 1061 992 83 Fragments arranged by size: 1061 992 83 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 29 208 1057 1896 2136 Size: 29 179 849 839 240 Fragments arranged by size: 849 839 240 179 29 AflII C'TTAA_G Cuts at: 0 1750 2136 Size: 1750 386 AhdI GACnn_n'nnGTC Cuts at: 0 1366 2136 Size: 1366 770 AleI CACnn'nnGTG Cuts at: 0 1567 2136 Size: 1567 569 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 707 2136 Size: 707 1429 ApoI r'AATT_y Cuts at: 0 1246 1344 1596 2136 Size: 1246 98 252 540 Fragments arranged by size: 1246 540 252 98 AvrII C'CTAG_G Cuts at: 0 1698 2136 Size: 1698 438 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1478 1511 2136 Size: 1478 33 625 Fragments arranged by size: 1478 625 33 BanII G_rGCy'C Cuts at: 0 1190 1413 2136 Size: 1190 223 723 Fragments arranged by size: 1190 723 223 BbsI GAAGACnn'nnnn_ Cuts at: 0 659 847 1902 2136 Size: 659 188 1055 234 Fragments arranged by size: 1055 659 234 188 BciVI GTATCCnnnnn_n' Cuts at: 0 1708 2136 Size: 1708 428 BclI T'GATC_A Cuts at: 0 960 2088 2136 Size: 960 1128 48 Fragments arranged by size: 1128 960 48 Bme1580I G_kGCm'C Cuts at: 0 1951 2136 Size: 1951 185 BmrI ACTGGGnnnn_n' Cuts at: 0 798 1051 1342 1954 2136 Size: 798 253 291 612 182 Fragments arranged by size: 798 612 291 253 182 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 440 2136 Size: 440 1696 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1149 1796 2136 Size: 1149 647 340 Fragments arranged by size: 1149 647 340 Bpu10I CC'TnA_GC Cuts at: 0 1966 2136 Size: 1966 170 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1416 2136 Size: 1416 720 BsaWI w'CCGG_w Cuts at: 0 1591 2136 Size: 1591 545 BseYI C'CCAG_C Cuts at: 0 1806 2136 Size: 1806 330 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 703 1545 2136 Size: 703 842 591 Fragments arranged by size: 842 703 591 BsiHKAI G_wGCw'C Cuts at: 0 196 1413 2136 Size: 196 1217 723 Fragments arranged by size: 1217 723 196 Bsp1286I G_dGCh'C Cuts at: 0 196 1190 1413 1951 2136 Size: 196 994 223 538 185 Fragments arranged by size: 994 538 223 196 185 BspEI T'CCGG_A Cuts at: 0 1591 2136 Size: 1591 545 BspHI T'CATG_A Cuts at: 0 1401 2136 Size: 1401 735 BsrDI GCAATG_nn' Cuts at: 0 386 623 1970 2136 Size: 386 237 1347 166 Fragments arranged by size: 1347 386 237 166 BsrGI T'GTAC_A Cuts at: 0 1078 2136 Size: 1078 1058 BstEII G'GTnAC_C Cuts at: 0 1303 2136 Size: 1303 833 BstXI CCAn_nnnn'nTGG Cuts at: 0 171 2136 Size: 171 1965 BstYI r'GATC_y Cuts at: 0 798 901 2136 Size: 798 103 1235 Fragments arranged by size: 1235 798 103 BstZ17I GTA'TAC Cuts at: 0 1062 2136 Size: 1062 1074 Bsu36I CC'TnA_GG Cuts at: 0 1602 2136 Size: 1602 534 BtsI GCAGTG_nn' Cuts at: 0 367 1211 2136 Size: 367 844 925 Fragments arranged by size: 925 844 367 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1515 1550 2136 Size: 1515 35 586 Fragments arranged by size: 1515 586 35 DraI TTT'AAA Cuts at: 0 75 1863 1991 2136 Size: 75 1788 128 145 Fragments arranged by size: 1788 145 128 75 EaeI y'GGCC_r Cuts at: 0 2113 2136 Size: 2113 23 EarI CTCTTCn'nnn_ Cuts at: 0 184 555 2136 Size: 184 371 1581 Fragments arranged by size: 1581 371 184 EcoICRI GAG'CTC Cuts at: 0 1411 2136 Size: 1411 725 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 29 208 1057 1149 1796 1896 2136 Size: 29 179 849 92 647 100 240 Fragments arranged by size: 849 647 240 179 100 92 29 EcoNI CCTnn'n_nnAGG Cuts at: 0 1527 2136 Size: 1527 609 EcoRV GAT'ATC Cuts at: 0 225 2136 Size: 225 1911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 86 150 2136 Size: 86 64 1986 Fragments arranged by size: 1986 86 64 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 27 532 564 1280 1312 1352 1384 1414 Size: 27 505 32 716 32 40 32 30 Cuts at: 1414 1446 2136 Size: 32 690 Fragments arranged by size: 716 690 505 40 32 32 32 32 30 27 HincII GTy'rAC Cuts at: 0 753 2136 Size: 753 1383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 39 342 1550 2136 Size: 39 303 1208 586 Fragments arranged by size: 1208 586 303 39 MscI TGG'CCA Cuts at: 0 2115 2136 Size: 2115 21 MslI CAynn'nnrTG Cuts at: 0 1567 2136 Size: 1567 569 MspA1I CmG'CkG Cuts at: 0 865 2136 Size: 865 1271 NdeI CA'TA_TG Cuts at: 0 1491 2136 Size: 1491 645 PasI CC'CwG_GG Cuts at: 0 984 2136 Size: 984 1152 PflMI CCAn_nnn'nTGG Cuts at: 0 115 712 2111 2136 Size: 115 597 1399 25 Fragments arranged by size: 1399 597 115 25 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1397 1429 2136 Size: 1397 32 707 Fragments arranged by size: 1397 707 32 PsiI TTA'TAA Cuts at: 0 286 2026 2136 Size: 286 1740 110 Fragments arranged by size: 1740 286 110 PvuII CAG'CTG Cuts at: 0 865 2136 Size: 865 1271 SacI G_AGCT'C Cuts at: 0 1413 2136 Size: 1413 723 SapI GCTCTTCn'nnn_ Cuts at: 0 184 555 2136 Size: 184 371 1581 Fragments arranged by size: 1581 371 184 ScaI AGT'ACT Cuts at: 0 1979 2136 Size: 1979 157 SmlI C'TyrA_G Cuts at: 0 1431 1750 2136 Size: 1431 319 386 Fragments arranged by size: 1431 386 319 SspI AAT'ATT Cuts at: 0 1220 1545 2136 Size: 1220 325 591 Fragments arranged by size: 1220 591 325 StuI AGG'CCT Cuts at: 0 66 2136 Size: 66 2070 StyI C'CwwG_G Cuts at: 0 1212 1377 1698 1763 2136 Size: 1212 165 321 65 373 Fragments arranged by size: 1212 373 321 165 65 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 665 2136 Size: 665 1471 TatI w'GTAC_w Cuts at: 0 404 1078 1133 1977 2136 Size: 404 674 55 844 159 Fragments arranged by size: 844 674 404 159 55 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1453 1485 1922 1954 2136 Size: 1453 32 437 32 182 Fragments arranged by size: 1453 437 182 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 615 2136 Size: 615 1521 XmnI GAAnn'nnTTC Cuts at: 0 66 1246 2136 Size: 66 1180 890 Fragments arranged by size: 1180 890 66 Enzymes that do cut: AccI AcuI AflII AhdI AleI AlfI ApoI AvrII BaeI BanII BbsI BciVI BclI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BsrDI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I BtsI CspCI DraI EaeI EarI EcoICRI Eco57MI EcoNI EcoRV FalI Hin4I HincII MmeI MscI MslI MspA1I NdeI PasI PflMI PpiI PsiI PvuII SacI SapI ScaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflIII AgeI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BglII BlpI BmgBI BmtI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoO109I EcoRI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XhoI XmaI ZraI