(Circular) (Six-base) MAPSORT of: pFN21AE0131.seq Check: 374 from: 1 to: 5254 pFN21AE0131 5254 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3064 278 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2597 1937 Size: 660 4594 Acc65I G'GTAC_C Cuts at: 2575 2575 Size: 5254 AclI AA'CG_TT Cuts at: 1563 3381 3754 1563 Size: 1818 373 3063 Fragments arranged by size: 3063 1818 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2175 3317 4701 4969 1632 Size: 543 1142 1384 268 1917 Fragments arranged by size: 1917 1384 1142 543 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5057 AflIII A'CryG_T Cuts at: 1203 4153 5130 1203 Size: 2950 977 1327 Fragments arranged by size: 2950 1327 977 AgeI A'CCGG_T Cuts at: 4085 4085 Size: 5254 AhdI GACnn_n'nnGTC Cuts at: 3983 3983 Size: 5254 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5254 AlwNI CAG_nnn'CTG Cuts at: 2997 4569 2997 Size: 1572 3682 ApaLI G'TGCA_C Cuts at: 3311 4467 3311 Size: 1156 4098 ApoI r'AATT_y Cuts at: 1493 2563 2789 2941 5050 1493 Size: 1070 226 152 2109 1697 Fragments arranged by size: 2109 1697 1070 226 152 AseI AT'TA_AT Cuts at: 160 1032 3808 160 Size: 872 2776 1606 Fragments arranged by size: 2776 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 AvaI C'yCGr_G Cuts at: 1870 1943 2136 2579 1870 Size: 73 193 443 4545 Fragments arranged by size: 4545 443 193 73 BamHI G'GATC_C Cuts at: 2584 2951 2584 Size: 367 4887 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2468 2478 2575 4030 Size: 325 217 627 354 327 10 97 1455 Cuts at: 4030 618 Size: 1842 Fragments arranged by size: 1842 1455 627 354 327 325 217 97 10 BanII G_rGCy'C Cuts at: 1391 2573 1391 Size: 1182 4072 BbeI G_GCGC'C Cuts at: 2472 2482 2472 Size: 10 5244 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2425 928 Size: 971 526 3757 Fragments arranged by size: 3757 971 526 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3445 3479 1903 Size: 34 1508 34 3678 Fragments arranged by size: 3678 1508 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3146 4356 3146 Size: 1210 4044 BclI T'GATC_A Cuts at: 1548 2496 1548 Size: 948 4306 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3865 136 Size: 107 122 71 631 605 2193 1525 Fragments arranged by size: 2193 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5249 5249 Size: 5254 BlpI GC'TnA_GC Cuts at: 2671 2671 Size: 5254 Bme1580I G_kGCm'C Cuts at: 1790 3315 4471 1790 Size: 1525 1156 2573 Fragments arranged by size: 2573 1525 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5254 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2985 3943 442 Size: 416 53 83 399 24 1568 958 1753 Fragments arranged by size: 1753 1568 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2282 2420 2477 3914 1239 Size: 1043 138 57 1437 2579 Fragments arranged by size: 2579 1437 1043 138 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3379 4244 4542 3379 Size: 865 298 4091 Fragments arranged by size: 4091 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 3917 882 Size: 617 984 1434 2219 Fragments arranged by size: 2219 1434 984 617 BsaAI yAC'GTr Cuts at: 493 1204 3045 493 Size: 711 1841 2702 Fragments arranged by size: 2702 1841 711 BsaBI GATnn'nnATC Cuts at: 2292 5248 2292 Size: 2956 2298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 2443 2469 2479 Size: 53 83 186 936 541 369 26 10 Cuts at: 2479 3061 3443 4078 275 Size: 582 382 635 1451 Fragments arranged by size: 1451 936 635 582 541 382 369 186 83 53 26 10 BsaWI w'CCGG_w Cuts at: 3687 4085 4359 4506 4917 3687 Size: 398 274 147 411 4024 Fragments arranged by size: 4024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5029 Fragments arranged by size: 5029 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5254 BseYI C'CCAG_C Cuts at: 2244 4457 4953 2244 Size: 2213 496 2545 Fragments arranged by size: 2545 2213 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4681 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2704 3465 4493 1540 Size: 275 186 703 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 275 186 BsiHKAI G_wGCw'C Cuts at: 2097 3315 3400 4471 2097 Size: 1218 85 1071 2880 Fragments arranged by size: 2880 1218 1071 85 BsmI GAATG_Cn' Cuts at: 2268 2765 2858 2268 Size: 497 93 4664 Fragments arranged by size: 4664 497 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2573 3315 3400 4471 1391 Size: 399 307 476 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 476 399 307 85 BspEI T'CCGG_A Cuts at: 4917 4917 Size: 5254 BspHI T'CATG_A Cuts at: 2412 3143 4873 2412 Size: 731 1730 2793 Fragments arranged by size: 2793 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2609 844 Size: 1765 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3141 1394 Size: 429 1318 3507 Fragments arranged by size: 3507 1318 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2523 3749 3923 65 Size: 1183 1275 1226 174 1396 Fragments arranged by size: 1396 1275 1226 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2439 3898 4085 1700 Size: 110 629 1459 187 2869 Fragments arranged by size: 2869 1459 629 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5254 BssHII G'CGCG_C Cuts at: 1925 2449 1925 Size: 524 4730 BssSI C'ACGA_G Cuts at: 3314 4326 3314 Size: 1012 4242 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5254 BstBI TT'CG_AA Cuts at: 4058 4058 Size: 5254 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5254 BstXI CCAn_nnnn'nTGG Cuts at: 2236 2236 Size: 5254 BstYI r'GATC_y Cuts at: 1972 2126 2584 2951 3336 3353 4805 4891 4903 Size: 154 458 367 385 17 1452 86 12 Cuts at: 4903 5249 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 154 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2281 513 Size: 552 939 277 3486 Fragments arranged by size: 3486 939 552 277 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2485 487 Size: 683 56 1259 3256 Fragments arranged by size: 3256 1259 683 56 BtsI GCAGTG_nn' Cuts at: 2370 2770 3564 3584 2370 Size: 400 794 20 4040 Fragments arranged by size: 4040 794 400 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5254 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5219 DraI TTT'AAA Cuts at: 2558 2558 Size: 5254 DraIII CAC_nnn'GTG Cuts at: 2101 2101 Size: 5254 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4261 809 Size: 507 2945 1802 Fragments arranged by size: 2945 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2517 2701 3590 Size: 54 1425 50 275 18 687 184 889 Cuts at: 3590 5219 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 687 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2701 1537 Size: 275 889 4090 Fragments arranged by size: 4090 889 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 3184 1353 Size: 1026 805 3423 Fragments arranged by size: 3423 1026 805 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 3841 4215 4361 387 Size: 1064 138 459 1793 374 146 1280 Fragments arranged by size: 1793 1280 1064 459 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2175 2282 2420 2477 3317 3914 4701 Size: 393 543 107 138 57 840 597 787 Cuts at: 4701 4969 1239 Size: 268 1524 Fragments arranged by size: 1524 840 787 597 543 393 268 138 107 57 EcoO109I rG'GnC_Cy Cuts at: 2015 2241 2015 Size: 226 5028 EcoRI G'AATT_C Cuts at: 2563 2941 2563 Size: 378 4876 FspI TGC'GCA Cuts at: 3760 3760 Size: 5254 HaeII r_GCGC'y Cuts at: 1678 2472 2482 4401 1678 Size: 794 10 1919 2531 Fragments arranged by size: 2531 1919 794 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2111 2143 3895 3927 3969 Size: 32 135 32 539 32 1752 32 42 Cuts at: 3969 4001 1373 Size: 32 2626 Fragments arranged by size: 2626 1752 539 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2437 2598 2844 669 Size: 988 281 499 161 246 3079 Fragments arranged by size: 3079 988 499 281 246 161 HindIII A'AGCT_T Cuts at: 748 748 Size: 5254 HpaI GTT'AAC Cuts at: 2844 2844 Size: 5254 KasI G'GCGC_C Cuts at: 2468 2478 2468 Size: 10 5244 KpnI G_GTAC'C Cuts at: 2579 2579 Size: 5254 MfeI C'AATT_G Cuts at: 2853 2853 Size: 5254 MluI A'CGCG_T Cuts at: 5130 5130 Size: 5254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2145 4368 4552 1305 Size: 840 2223 184 2007 Fragments arranged by size: 2223 2007 840 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3432 Fragments arranged by size: 3432 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2286 2504 3212 3571 3730 518 Size: 648 1120 218 708 359 159 2042 Fragments arranged by size: 2042 1120 708 648 359 218 159 MspA1I CmG'CkG Cuts at: 1606 2248 2425 2670 3347 4495 4740 1606 Size: 642 177 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 642 245 245 177 NarI GG'CG_CC Cuts at: 2469 2479 2469 Size: 10 5244 NcoI C'CATG_G Cuts at: 513 1065 2004 2281 513 Size: 552 939 277 3486 Fragments arranged by size: 3486 939 552 277 NdeI CA'TA_TG Cuts at: 387 387 Size: 5254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5254 NotI GC'GGCC_GC Cuts at: 2701 2701 Size: 5254 NspI r_CATG'y Cuts at: 1132 1229 2612 4157 1132 Size: 97 1383 1545 2229 Fragments arranged by size: 2229 1545 1383 97 PasI CC'CwG_GG Cuts at: 1348 2351 1348 Size: 1003 4251 PciI A'CATG_T Cuts at: 4153 4153 Size: 5254 PflMI CCAn_nnn'nTGG Cuts at: 1108 2535 1108 Size: 1427 3827 PfoI T'CCnGG_A Cuts at: 2298 2298 Size: 5254 PmeI GTTT'AAAC Cuts at: 2558 2558 Size: 5254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3323 3355 4836 4868 3323 Size: 32 1481 32 3709 Fragments arranged by size: 3709 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2241 2241 Size: 5254 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5254 PsiI TTA'TAA Cuts at: 2824 2824 Size: 5254 PstI C_TGCA'G Cuts at: 830 2606 830 Size: 1776 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5254 PvuII CAG'CTG Cuts at: 2248 2425 2248 Size: 177 5077 SalI G'TCGA_C Cuts at: 1936 2596 1936 Size: 660 4594 SanDI GG'GwC_CC Cuts at: 2241 2241 Size: 5254 SbfI CC_TGCA'GG Cuts at: 2606 2606 Size: 5254 ScaI AGT'ACT Cuts at: 3502 5214 3502 Size: 1712 3542 SexAI A'CCwGG_T Cuts at: 2097 2550 2097 Size: 453 4801 SfcI C'TryA_G Cuts at: 826 1045 2602 3737 4418 4609 826 Size: 219 1557 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2470 2480 2470 Size: 10 5244 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5254 SmaI CCC'GGG Cuts at: 1872 2138 2581 1872 Size: 266 443 4545 Fragments arranged by size: 4545 443 266 SmlI C'TyrA_G Cuts at: 820 1017 1943 3358 4259 4521 820 Size: 197 926 1415 901 262 1553 Fragments arranged by size: 1553 1415 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3045 493 Size: 2552 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5254 SphI G_CATG'C Cuts at: 1132 2612 1132 Size: 1480 3774 SspI AAT'ATT Cuts at: 5 52 3178 5 Size: 47 3126 2081 Fragments arranged by size: 3126 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5254 StyI C'CwwG_G Cuts at: 513 1065 2004 2028 2077 2281 2320 2693 513 Size: 552 939 24 49 204 39 373 3074 Fragments arranged by size: 3074 939 552 373 204 49 39 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2121 3281 3298 3451 3636 4081 325 Size: 1255 541 1160 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1160 541 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2395 3500 5212 Size: 274 80 33 51 1443 418 1105 1712 Cuts at: 5212 96 Size: 138 Fragments arranged by size: 1712 1443 1105 418 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5214 5246 5214 Size: 32 5222 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5254 XbaI T'CTAG_A Cuts at: 2590 2590 Size: 5254 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 2306 2035 Size: 271 4983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5254 XmaI C'CCGG_G Cuts at: 1870 2136 2579 1870 Size: 266 443 4545 Fragments arranged by size: 4545 443 266 XmnI GAAnn'nnTTC Cuts at: 3383 3383 Size: 5254 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3062 276 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI