Restriction map for
pFN21AB5399
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# NO CUT ENZYMES ( 177 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsiYI BslI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT14I
EcoT22I FauI FseI FspI HaeII
HapII HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 451 GTAGAC GT^[AC][GT]AC
AfaI 90 GTAC GT^AC
AlwNI 295 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 54 GAGCAC G[AT]GC[AT]^C
AvaII 42 GGACC G^G[AT]CC
BanI 232 GGCACC G^G[CT][GA]CC
BclI 404 TGATCA T^GATCA
BmyI 54 GAGCAC G[AGT]GC[ACT]^C
BsaJI 278 CCAGGG C^C[ACGT][ACGT]GG
BsiHKAI 54 GAGCAC G[AT]GC[AT]^C
BsmAI 176 GAGAC GTCTC(1/5)
BsmBI 176 GAGACG CGTCTC(1/5)
BsmFI 227 GGGAC GGGAC(10/14)
Bsp1286I 54 GAGCAC G[AGT]GC[ACT]^C
BspHI 407 TCATGA T^CATGA
Cac8I 198 GCATGC GC[ACGT]^[ACGT]GC
CfoI 260 GCGC GCG^C
DdeI 440 CTGAG C^T[ACGT]AG
DraII 41 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 51 GAAGAG CTCTTC(1/4)
EcoO109I 41 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 508 GAATTC G^AATTC
FbaI 404 TGATCA T^GATCA
HhaI 260 GCGC GCG^C
HinP1I 260 GCGC G^CGC
Ksp632I 51 GAAGAG CTCTTC(1/4)
MboII 51 GAAGA GAAGA(8/7)
MseI 65 TTAA T^TAA
MslI 348 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 198 GCATGC [GA]CATG^[CT]
PaeR7I 133 CTCGAG C^TCGAG
PpuMI 41 AGGACCT [GA]G^G[AT]CC[CT]
PstI 553 CTGCAG CTGCA^G
RcaI 407 TCATGA T^CATGA
RsaI 90 GTAC GT^AC
SapI 51 GAAGAGC GCTCTTC(1/4)
SexAI 44 ACCTGGT A^CC[AT]GGT
SfaNI 360 GATGC GCATC(5/9)
SfcI 553 CTGCAG C^T[GA][CT]AG
SphI 198 GCATGC GCATG^C
Tru9I 65 TTAA T^TAA
XhoI 133 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AcsI 139 GAATTT [GA]^AATT[CT]
508 GAATTC
AcyI 178 GACGCC G[GA]^CG[CT]C
435 GACGCC
ApoI 139 GAATTT [GA]^AATT[CT]
508 GAATTC
AvaI 133 CTCGAG C^[CT]CG[GA]G
350 CCCGAG
BpmI 276 CTCCAG CTGGAG(16/14)
364 CTGGAG
BsaHI 178 GACGCC G[GA]^CG[CT]C
435 GACGCC
BsoBI 133 CTCGAG C^[CT]CG[GA]G
350 CCCGAG
HgaI 178 GACGC GACGC(5/10)
435 GACGC
Hin1I 178 GACGCC G[GA]^CG[CT]C
435 GACGCC
MwoI 193 GCCCAGCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
438 GCCTGAGCAGC
NlaIV 24 GGCCCC GG[ACGT]^[ACGT]CC
232 GGCACC
Tsp509I 140 AATT ^AATT
509 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BbvI BsrI BssKI BstF5I
BstNI Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HphI ItaI MboI
MnlI MvaI NdeII NlaIII Sau3AI
Sau96I ScrFI SmlI TaqI TseI
TspRI TthHB8I