################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI StyI SwaI Tru9I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 466 GTCTAC GT^[AC][GT]AC AlwNI 309 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 148 CCTGAGG CC^T[ACGT]AGG Aor51HI 59 AGCGCT AGC^GCT ApaI 530 GGGCCC GGGCC^C AspHI 235 GTGCTC G[AT]GC[AT]^C AspI 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 590 GGTGCC G^G[CT][GA]CC BcnI 533 CCCGG CC^[GC]GG BpmI 63 CTGGAG CTGGAG(16/14) BsaI 655 GAGACC GGTCTC(1/5) BseRI 201 CTCCTC GAGGAG(10/8) BsgI 626 GTGCAG GTGCAG(16/14) BsiHKAI 235 GTGCTC G[AT]GC[AT]^C BsmAI 655 GAGAC GTCTC(1/5) BsrI 114 CCAGT ACTGG(1/-1) BstF5I 69 CATCC GGATG(2/0) BstXI 554 CCATAATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 148 CCTGAGG CC^T[ACGT]AGG DpnII 816 GATC ^GATC DraII 529 AGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 676 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 59 AGCGCT AGC^GCT Eco81I 148 CCTGAGG CC^T[ACGT]AGG EcoO109I 529 AGGGCCC [GA]G^G[ACGT]CC[CT] FauI 726 GCGGG CCCGC(4/6) FokI 69 CATCC GGATG(9/13) HapII 534 CCGG C^CGG HgaI 451 GACGC GACGC(5/10) HpaII 534 CCGG C^CGG HphI 17 TCACC GGTGA(8/7) MaeII 680 ACGT A^CGT MboI 816 GATC ^GATC MspI 534 CCGG C^CGG NciI 533 CCCGG CC^[GC]GG NdeI 338 CATATG CA^TATG NdeII 816 GATC ^GATC PflFI 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 73 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 662 CTGCAG CTGCA^G Sau3AI 816 GATC ^GATC SfcI 662 CTGCAG C^T[GA][CT]AG SmlI 853 CTCAAG C^T[CT][GA]AG TfiI 515 GAATC G^A[AT]TC Tsp509I 558 AATT ^AATT Tth111I 190 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 73 CCAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AfaI 117 GTAC GT^AC 883 GTAC BalI 272 TGGCCA TGG^CCA 821 TGGCCA BanII 106 GAGCCC G[GA]GC[CT]^C 530 GGGCCC BsaJI 27 CCAGGG C^C[ACGT][ACGT]GG 787 CCCAGG BsiYI 73 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 720 CCTTCGGCGGG BslI 73 CCAGCTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 720 CCTTCGGCGGG BsmFI 449 GGGAC GGGAC(10/14) 728 GGGAC BspMI 286 GCAGGT ACCTGC(4/8) 441 GCAGGT EaeI 272 TGGCCA [CT]^GGCC[GA] 821 TGGCCA HaeII 59 AGCGCT [GA]GCGC^[CT] 800 GGCGCT MaeIII 16 GTCAC ^GT[ACGT]AC 578 GTGAC MboII 331 GAAGA GAAGA(8/7) 741 GAAGA MluNI 272 TGGCCA TGG^CCA 821 TGGCCA MscI 272 TGGCCA TGG^CCA 821 TGGCCA MspA1I 74 CAGCTG C[AC]G^C[GT]G 665 CAGCTG PvuII 74 CAGCTG CAG^CTG 665 CAGCTG RsaI 117 GTAC GT^AC 883 GTAC TaqI 494 TCGA T^CGA 653 TCGA Tsp45I 16 GTCAC ^GT[GC]AC 578 GTGAC TthHB8I 494 TCGA T^CGA 653 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AvaII BbvI BcgI BmyI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MnlI MvaI MwoI NlaIII NlaIV NspI PleI Sau96I ScrFI SfaNI SphI TseI TspRI