################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII AccI AclI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspI AviII AvrII BbrPI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BsaI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI Eco47III EcoO65I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MaeI MfeI MluI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 1507 GGTACC G^GTACC AccII 1165 CGCG CG^CG AccIII 1007 TCCGGA T^CCGGA AflIII 1358 ACGTGT A^C[GA][CT]GT AocI 1209 CCTCAGG CC^T[ACGT]AGG ApaI 234 GGGCCC GGGCC^C Asp718I 1507 GGTACC G^GTACC AspHI 226 GAGCTC G[AT]GC[AT]^C BamHI 1481 GGATCC G^GATCC BbsI 1494 GAAGAC GAAGAC(2/6) BcgI 1607 GCATCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 31 AGATCT A^GATCT BpuAI 1494 GAAGAC GAAGAC(2/6) BsaAI 778 TACGTG [CT]AC^GT[GA] BsaBI 399 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1007 TCCGGA T^CCGGA BsgI 928 GTGCAG GTGCAG(16/14) BsiEI 245 CGGCCG CG[GA][CT]^CG BsiHKAI 226 GAGCTC G[AT]GC[AT]^C BspEI 1007 TCCGGA T^CCGGA BspHI 1082 TCATGA T^CATGA BsrBI 864 GAGCGG CCGCTC(-3/-3) BsrI 1101 CCAGT ACTGG(1/-1) BssSI 1456 CACGAG CACGAG(-5/-1) BstUI 1165 CGCG CG^CG Bsu36I 1209 CCTCAGG CC^T[ACGT]AGG DraIII 1532 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1159 GACACCCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1658 CCATGG C^C[GA][CT]GG EagI 245 CGGCCG C^GGCCG EarI 898 CTCTTC CTCTTC(1/4) EclXI 245 CGGCCG C^GGCCG Eco52I 245 CGGCCG C^GGCCG Eco81I 1209 CCTCAGG CC^T[ACGT]AGG EcoRI 573 GAATTC G^AATTC KpnI 1507 GGTACC GGTAC^C Ksp632I 898 CTCTTC CTCTTC(1/4) MaeIII 1451 GTGAC ^GT[ACGT]AC MamI 399 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1007 TCCGGA T^CCGGA MseI 886 TTAA T^TAA MvnI 1165 CGCG CG^CG NcoI 1658 CCATGG C^CATGG NspI 1577 ACATGC [GA]CATG^[CT] RcaI 1082 TCATGA T^CATGA SacI 226 GAGCTC GAGCT^C SbfI 1116 CCTGCAGG CCTGCA^GG SexAI 1463 ACCAGGT A^CC[AT]GGT Sse8387I 1116 CCTGCAGG CCTGCA^GG Tru9I 886 TTAA T^TAA Tsp45I 1451 GTGAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AhdI 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1336 GACCTGCTGTC AlwNI 136 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 578 CAGAGCCTG AspEI 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1336 GACCTGCTGTC AvaI 231 CTCGGG C^[CT]CG[GA]G 463 CCCGAG BalI 113 TGGCCA TGG^CCA 1661 TGGCCA BbeI 537 GGCGCC GGCGC^C 735 GGCGCC BbvCI 532 GCTGAGG CCTCAGC(-5/-2) 640 GCTGAGG BglI 106 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 667 GCCACTGAGGC BsmAI 55 GAGAC GTCTC(1/5) 594 GTCTC BsoBI 231 CTCGGG C^[CT]CG[GA]G 463 CCCGAG Eam1105I 283 GACATCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1336 GACCTGCTGTC KasI 537 GGCGCC G^GCGCC 735 GGCGCC MaeII 779 ACGT A^CGT 1358 ACGT MluNI 113 TGGCCA TGG^CCA 1661 TGGCCA MscI 113 TGGCCA TGG^CCA 1661 TGGCCA MslI 846 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1353 CATCGACGTG NarI 537 GGCGCC GG^CGCC 735 GGCGCC PflMI 135 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 561 CCAGACCTTGG PstI 601 CTGCAG CTGCA^G 1117 CTGCAG PvuII 598 CAGCTG CAG^CTG 684 CAGCTG SfcI 601 CTGCAG C^T[GA][CT]AG 1117 CTGCAG SfoI 537 GGCGCC GGC^GCC 735 GGCGCC SmlI 340 CTCAAG C^T[CT][GA]AG 727 CTCAAG Van91I 135 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 561 CCAGACCTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI ApoI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsaWI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TspRI TthHB8I XhoII