(Circular) (Six-base) MAPSORT of: pFN21AE4525.seq Check: 2215 from: 1 to: 6409 pFN21AE4525 6409 bp With 182 enzymes: SgfI * March 28, 2012 14:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2293 2293 Size: 6409 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4219 278 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3752 1937 Size: 1815 4594 Acc65I G'GTAC_C Cuts at: 3512 3730 3512 Size: 218 6191 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4472 5856 6124 1632 Size: 2840 1384 268 1917 Fragments arranged by size: 2840 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6212 AflIII A'CryG_T Cuts at: 1203 3363 5308 6285 1203 Size: 2160 1945 977 1327 Fragments arranged by size: 2160 1945 1327 977 AgeI A'CCGG_T Cuts at: 5240 5240 Size: 6409 AhdI GACnn_n'nnGTC Cuts at: 2293 3346 5138 2293 Size: 1053 1792 3564 Fragments arranged by size: 3564 1792 1053 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2760 1184 Size: 1576 4833 AlwNI CAG_nnn'CTG Cuts at: 2146 2588 4152 5724 2146 Size: 442 1564 1572 2831 Fragments arranged by size: 2831 1572 1564 442 ApaI G_GGCC'C Cuts at: 2243 2243 Size: 6409 ApaLI G'TGCA_C Cuts at: 4466 5622 4466 Size: 1156 5253 ApoI r'AATT_y Cuts at: 1493 2135 2578 3380 3718 3944 4096 6205 1493 Size: 642 443 802 338 226 152 2109 1697 Fragments arranged by size: 2109 1697 802 642 443 338 226 152 AseI AT'TA_AT Cuts at: 160 1032 4963 160 Size: 872 3931 1606 Fragments arranged by size: 3931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 2236 2468 3734 1870 Size: 73 293 232 1266 4545 Fragments arranged by size: 4545 1266 293 232 73 BamHI G'GATC_C Cuts at: 3486 3739 4106 3486 Size: 253 367 5789 Fragments arranged by size: 5789 367 253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2473 2542 2740 3512 3730 Size: 325 217 627 686 69 198 772 218 Cuts at: 3730 5185 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 772 686 627 325 218 217 198 69 BanII G_rGCy'C Cuts at: 1391 2235 2243 2346 2451 2625 2733 3728 1391 Size: 844 8 103 105 174 108 995 4072 Fragments arranged by size: 4072 995 844 174 108 105 103 8 BbeI G_GCGC'C Cuts at: 2546 2744 2546 Size: 198 6211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3506 928 Size: 971 1607 3831 Fragments arranged by size: 3831 1607 971 BbvCI CC'TCA_GC Cuts at: 2538 2646 2538 Size: 108 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3601 3635 4600 4634 1903 Size: 34 1664 34 965 34 3678 Fragments arranged by size: 3678 1664 965 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4301 5511 4301 Size: 1210 5199 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2117 2678 5020 Size: 107 122 71 631 605 445 561 2342 Cuts at: 5020 136 Size: 1525 Fragments arranged by size: 2342 1525 631 605 561 445 122 107 71 BglII A'GATC_T Cuts at: 2036 6404 2036 Size: 4368 2041 BlpI GC'TnA_GC Cuts at: 3826 3826 Size: 6409 Bme1580I G_kGCm'C Cuts at: 1790 2243 2478 4470 5626 1790 Size: 453 235 1992 1156 2573 Fragments arranged by size: 2573 1992 1156 453 235 BmgBI CAC'GTC Cuts at: 1318 3364 1318 Size: 2046 4363 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4140 5098 442 Size: 416 53 83 399 24 2723 958 1753 Fragments arranged by size: 2723 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2639 2825 3323 5069 1239 Size: 1400 186 498 1746 2579 Fragments arranged by size: 2579 1746 1400 498 186 Bpu10I CC'TnA_GC Cuts at: 2192 2538 2646 3248 2192 Size: 346 108 602 5353 Fragments arranged by size: 5353 602 346 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 2717 4534 5399 5697 2330 Size: 387 1817 865 298 3042 Fragments arranged by size: 3042 1817 865 387 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5072 882 Size: 617 3573 2219 Fragments arranged by size: 3573 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2785 4200 493 Size: 711 1581 1415 2702 Fragments arranged by size: 2702 1581 1415 711 BsaBI GATnn'nnATC Cuts at: 2408 6403 2408 Size: 3995 2414 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2213 2375 2543 2741 Size: 53 83 186 936 680 162 168 198 Cuts at: 2741 3009 4216 4598 5233 275 Size: 268 1207 382 635 1451 Fragments arranged by size: 1451 1207 936 680 635 382 268 198 186 168 162 83 53 BsaWI w'CCGG_w Cuts at: 2016 2436 3012 4842 5240 5514 5661 6072 2016 Size: 420 576 1830 398 274 147 411 2353 Fragments arranged by size: 2353 1830 576 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6184 Fragments arranged by size: 6184 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2963 3014 3143 3320 3695 1186 Size: 880 897 51 129 177 375 3900 Fragments arranged by size: 3900 897 880 375 177 129 51 BseYI C'CCAG_C Cuts at: 2555 2560 2631 2687 5612 6108 2555 Size: 5 71 56 2925 496 2856 Fragments arranged by size: 2925 2856 496 71 56 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 1163 Size: 573 1218 4618 Fragments arranged by size: 4618 1218 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2253 3859 4620 5648 1540 Size: 275 186 252 1606 761 1028 2301 Fragments arranged by size: 2301 1606 1028 761 275 252 186 BsiHKAI G_wGCw'C Cuts at: 2235 4470 4555 5626 2235 Size: 2235 85 1071 3018 Fragments arranged by size: 3018 2235 1071 85 BsmI GAATG_Cn' Cuts at: 3920 4013 3920 Size: 93 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2243 2346 2451 2478 2625 2733 Size: 399 445 8 103 105 27 147 108 Cuts at: 2733 3728 4470 4555 5626 1391 Size: 995 742 85 1071 2174 Fragments arranged by size: 2174 1071 995 742 445 399 147 108 105 103 85 27 8 BspEI T'CCGG_A Cuts at: 3012 6072 3012 Size: 3060 3349 BspHI T'CATG_A Cuts at: 3087 4298 6028 3087 Size: 1211 1730 3468 Fragments arranged by size: 3468 1730 1211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2293 3129 3351 3564 3764 844 Size: 1449 836 222 213 200 3489 Fragments arranged by size: 3489 1449 836 222 213 200 BsrBI CCG'CTC Cuts at: 1394 1823 2871 4296 1394 Size: 429 1048 1425 3507 Fragments arranged by size: 3507 1425 1048 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2257 2383 3203 3402 5053 5240 1700 Size: 110 447 126 820 199 1651 187 2869 Fragments arranged by size: 2869 1651 820 447 199 187 126 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6409 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6409 BssSI C'ACGA_G Cuts at: 3461 4469 5481 3461 Size: 1008 1012 4389 Fragments arranged by size: 4389 1012 1008 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6409 BstBI TT'CG_AA Cuts at: 5213 5213 Size: 6409 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6409 BstXI CCAn_nnnn'nTGG Cuts at: 2305 2512 3676 2305 Size: 207 1164 5038 Fragments arranged by size: 5038 1164 207 BstYI r'GATC_y Cuts at: 1972 2019 2036 2517 3486 3739 4106 4491 4508 Size: 47 17 481 969 253 367 385 17 Cuts at: 4508 5960 6046 6058 6404 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 969 481 385 367 346 253 86 47 17 17 12 Bsu36I CC'TnA_GG Cuts at: 3215 3215 Size: 6409 BtgI C'CryG_G Cuts at: 513 1065 2004 3663 513 Size: 552 939 1659 3259 Fragments arranged by size: 3259 1659 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3262 487 Size: 683 56 2036 3634 Fragments arranged by size: 3634 2036 683 56 BtsI GCAGTG_nn' Cuts at: 2478 3175 3460 3925 4719 4739 2478 Size: 697 285 465 794 20 4148 Fragments arranged by size: 4148 794 697 465 285 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6374 DraI TTT'AAA Cuts at: 3713 3713 Size: 6409 DraIII CAC_nnn'GTG Cuts at: 3542 3542 Size: 6409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3170 5416 809 Size: 507 1854 2246 1802 Fragments arranged by size: 2246 1854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2250 2706 Size: 54 1425 50 275 18 288 132 456 Cuts at: 2706 3205 3666 3856 4745 6374 8 Size: 499 461 190 889 1629 43 Fragments arranged by size: 1629 1425 889 499 461 456 288 275 190 132 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2250 3856 1537 Size: 275 438 1606 4090 Fragments arranged by size: 4090 1606 438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 4339 1353 Size: 1556 1430 3423 Fragments arranged by size: 3423 1556 1430 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3302 4996 5370 5516 387 Size: 1064 138 1713 1694 374 146 1280 Fragments arranged by size: 1713 1694 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2233 Size: 6409 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2639 2825 3323 4472 5069 5856 6124 Size: 393 1007 186 498 1149 597 787 268 Cuts at: 6124 1239 Size: 1524 Fragments arranged by size: 1524 1149 1007 787 597 498 393 268 186 EcoNI CCTnn'n_nnAGG Cuts at: 3125 3251 3647 3125 Size: 126 396 5887 Fragments arranged by size: 5887 396 126 EcoO109I rG'GnC_Cy Cuts at: 2143 2240 2464 2764 3219 3562 2143 Size: 97 224 300 455 343 4990 Fragments arranged by size: 4990 455 343 300 224 97 EcoRI G'AATT_C Cuts at: 2578 3718 4096 2578 Size: 1140 378 4891 Fragments arranged by size: 4891 1140 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2854 2854 Size: 6409 FspI TGC'GCA Cuts at: 4915 4915 Size: 6409 HaeII r_GCGC'y Cuts at: 1678 2546 2744 3643 3663 5556 1678 Size: 868 198 899 20 1893 2531 Fragments arranged by size: 2531 1893 899 868 198 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2006 2038 2246 2278 2279 Size: 32 135 32 434 32 208 32 1 Cuts at: 2279 2311 2963 2995 3332 3364 3377 3409 5050 Size: 32 652 32 337 32 13 32 1641 Cuts at: 5050 5082 5124 5156 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1641 652 434 337 208 135 42 32 32 32 32 32 32 32 32 32 32 13 1 HincII GTy'rAC Cuts at: 669 1657 1938 3136 3262 3475 3753 3999 669 Size: 988 281 1198 126 213 278 246 3079 Fragments arranged by size: 3079 1198 988 281 278 246 213 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 6409 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KasI G'GCGC_C Cuts at: 2542 2740 2542 Size: 198 6211 KpnI G_GTAC'C Cuts at: 3516 3734 3516 Size: 218 6191 MfeI C'AATT_G Cuts at: 4008 4008 Size: 6409 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3375 5523 5707 1305 Size: 2070 2148 184 2007 Fragments arranged by size: 2148 2070 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 3668 10 Size: 54 1425 343 288 1548 2751 Fragments arranged by size: 2751 1548 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2855 3362 4367 4726 4885 518 Size: 648 1689 507 1005 359 159 2042 Fragments arranged by size: 2042 1689 1005 648 507 359 159 MspA1I CmG'CkG Cuts at: 1606 2605 2691 3328 3825 4502 5650 5895 1606 Size: 999 86 637 497 677 1148 245 2120 Fragments arranged by size: 2120 1148 999 677 637 497 245 86 NarI GG'CG_CC Cuts at: 2543 2741 2543 Size: 198 6211 NcoI C'CATG_G Cuts at: 513 1065 2004 3663 513 Size: 552 939 1659 3259 Fragments arranged by size: 3259 1659 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6409 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 3586 3767 5312 1132 Size: 97 2357 181 1545 2229 Fragments arranged by size: 2357 2229 1545 181 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6409 PciI A'CATG_T Cuts at: 5308 5308 Size: 6409 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 2572 1108 Size: 1038 426 4945 Fragments arranged by size: 4945 1038 426 PfoI T'CCnGG_A Cuts at: 2139 2802 3159 3423 2139 Size: 663 357 264 5125 Fragments arranged by size: 5125 663 357 264 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2464 3562 2143 Size: 321 1098 4990 Fragments arranged by size: 4990 1098 321 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6409 PsiI TTA'TAA Cuts at: 3979 3979 Size: 6409 PspOMI G'GGCC_C Cuts at: 2239 2239 Size: 6409 PstI C_TGCA'G Cuts at: 830 2610 3126 3761 830 Size: 1780 516 635 3478 Fragments arranged by size: 3478 1780 635 516 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 PvuII CAG'CTG Cuts at: 2605 2691 2605 Size: 86 6323 SacI G_AGCT'C Cuts at: 2235 2235 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SbfI CC_TGCA'GG Cuts at: 3126 3761 3126 Size: 635 5774 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SexAI A'CCwGG_T Cuts at: 3468 3468 Size: 6409 SfcI C'TryA_G Cuts at: 826 1045 2606 3122 3757 4892 5573 5764 826 Size: 219 1561 516 635 1135 681 191 1471 Fragments arranged by size: 1561 1471 1135 681 635 516 219 191 SfoI GGC'GCC Cuts at: 2544 2742 2544 Size: 198 6211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 3736 1872 Size: 1864 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 2732 4513 5414 5676 820 Size: 197 926 402 387 1781 901 262 1553 Fragments arranged by size: 1781 1553 926 901 402 387 262 197 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6409 SphI G_CATG'C Cuts at: 1132 3767 1132 Size: 2635 3774 SspI AAT'ATT Cuts at: 5 52 4333 5 Size: 47 4281 2081 Fragments arranged by size: 4281 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6409 StyI C'CwwG_G Cuts at: 513 1065 2004 2208 2244 2460 2571 3282 3490 Size: 552 939 204 36 216 111 711 208 Cuts at: 3490 3663 3848 513 Size: 173 185 3074 Fragments arranged by size: 3074 939 711 552 216 208 204 185 173 111 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2080 2144 4436 4453 4606 4791 5236 Size: 1255 500 64 2292 17 153 185 445 Cuts at: 5236 325 Size: 1498 Fragments arranged by size: 2292 1498 1255 500 445 185 153 64 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4655 6367 96 Size: 274 80 33 51 1443 2678 1712 138 Fragments arranged by size: 2678 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6369 6401 6369 Size: 32 6377 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6409 XbaI T'CTAG_A Cuts at: 3745 3745 Size: 6409 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 3734 1870 Size: 1864 4545 XmnI GAAnn'nnTTC Cuts at: 4538 4538 Size: 6409 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4217 276 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI XcmI