(Linear) (Six-base) MAPSORT of: KIEE4525.seq Check: 6890 from: 1 to: 1704 KIEE4525.orf With 182 enzymes: SgfI * March 22, 2012 18:16 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 288 1704 Size: 288 1416 Acc65I G'GTAC_C Cuts at: 0 1507 1704 Size: 1507 197 AflIII A'CryG_T Cuts at: 0 1358 1704 Size: 1358 346 AhdI GACnn_n'nnGTC Cuts at: 0 288 1341 1704 Size: 288 1053 363 Fragments arranged by size: 1053 363 288 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 755 1704 Size: 755 949 AlwNI CAG_nnn'CTG Cuts at: 0 141 583 1704 Size: 141 442 1121 Fragments arranged by size: 1121 442 141 ApaI G_GGCC'C Cuts at: 0 238 1704 Size: 238 1466 ApoI r'AATT_y Cuts at: 0 130 573 1375 1704 Size: 130 443 802 329 Fragments arranged by size: 802 443 329 130 AvaI C'yCGr_G Cuts at: 0 231 463 1704 Size: 231 232 1241 Fragments arranged by size: 1241 232 231 BamHI G'GATC_C Cuts at: 0 1481 1704 Size: 1481 223 BanI G'GyrC_C Cuts at: 0 468 537 735 1507 1704 Size: 468 69 198 772 197 Fragments arranged by size: 772 468 198 197 69 BanII G_rGCy'C Cuts at: 0 230 238 341 446 620 728 1704 Size: 230 8 103 105 174 108 976 Fragments arranged by size: 976 230 174 108 105 103 8 BbeI G_GCGC'C Cuts at: 0 541 739 1704 Size: 541 198 965 Fragments arranged by size: 965 541 198 BbsI GAAGACnn'nnnn_ Cuts at: 0 1501 1704 Size: 1501 203 BbvCI CC'TCA_GC Cuts at: 0 533 641 1704 Size: 533 108 1063 Fragments arranged by size: 1063 533 108 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1596 1630 1704 Size: 1596 34 74 Fragments arranged by size: 1596 74 34 BglI GCCn_nnn'nGGC Cuts at: 0 112 673 1704 Size: 112 561 1031 Fragments arranged by size: 1031 561 112 BglII A'GATC_T Cuts at: 0 31 1704 Size: 31 1673 Bme1580I G_kGCm'C Cuts at: 0 238 473 1704 Size: 238 235 1231 Fragments arranged by size: 1231 238 235 BmgBI CAC'GTC Cuts at: 0 1359 1704 Size: 1359 345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 820 1318 1704 Size: 634 186 498 386 Fragments arranged by size: 634 498 386 186 Bpu10I CC'TnA_GC Cuts at: 0 187 533 641 1243 1704 Size: 187 346 108 602 461 Fragments arranged by size: 602 461 346 187 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 712 1704 Size: 325 387 992 Fragments arranged by size: 992 387 325 BsaAI yAC'GTr Cuts at: 0 780 1704 Size: 780 924 BsaBI GATnn'nnATC Cuts at: 0 403 1704 Size: 403 1301 BsaHI Gr'CG_yC Cuts at: 0 208 370 538 736 1004 1704 Size: 208 162 168 198 268 700 Fragments arranged by size: 700 268 208 198 168 162 BsaWI w'CCGG_w Cuts at: 0 11 431 1007 1704 Size: 11 420 576 697 Fragments arranged by size: 697 576 420 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 958 1009 1138 1315 1690 1704 Size: 61 897 51 129 177 375 14 Fragments arranged by size: 897 375 177 129 61 51 14 BseYI C'CCAG_C Cuts at: 0 550 555 626 682 1704 Size: 550 5 71 56 1022 Fragments arranged by size: 1022 550 71 56 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 949 1704 Size: 949 755 BsiEI CG_ry'CG Cuts at: 0 248 1704 Size: 248 1456 BsiHKAI G_wGCw'C Cuts at: 0 230 1704 Size: 230 1474 Bsp1286I G_dGCh'C Cuts at: 0 230 238 341 446 473 620 728 1704 Size: 230 8 103 105 27 147 108 976 Fragments arranged by size: 976 230 147 108 105 103 27 8 BspEI T'CCGG_A Cuts at: 0 1007 1704 Size: 1007 697 BspHI T'CATG_A Cuts at: 0 1082 1704 Size: 1082 622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 288 1124 1346 1559 1704 Size: 288 836 222 213 145 Fragments arranged by size: 836 288 222 213 145 BsrBI CCG'CTC Cuts at: 0 866 1704 Size: 866 838 BsrFI r'CCGG_y Cuts at: 0 252 378 1198 1397 1704 Size: 252 126 820 199 307 Fragments arranged by size: 820 307 252 199 126 BssSI C'ACGA_G Cuts at: 0 1456 1704 Size: 1456 248 BstXI CCAn_nnnn'nTGG Cuts at: 0 300 507 1671 1704 Size: 300 207 1164 33 Fragments arranged by size: 1164 300 207 33 BstYI r'GATC_y Cuts at: 0 14 31 512 1481 1704 Size: 14 17 481 969 223 Fragments arranged by size: 969 481 223 17 14 Bsu36I CC'TnA_GG Cuts at: 0 1210 1704 Size: 1210 494 BtgI C'CryG_G Cuts at: 0 1658 1704 Size: 1658 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1257 1704 Size: 1257 447 BtsI GCAGTG_nn' Cuts at: 0 473 1170 1455 1704 Size: 473 697 285 249 Fragments arranged by size: 697 473 285 249 DraIII CAC_nnn'GTG Cuts at: 0 1537 1704 Size: 1537 167 DrdI GACnn_nn'nnGTC Cuts at: 0 1165 1704 Size: 1165 539 EaeI y'GGCC_r Cuts at: 0 113 245 701 1200 1661 1704 Size: 113 132 456 499 461 43 Fragments arranged by size: 499 461 456 132 113 43 EagI C'GGCC_G Cuts at: 0 245 1704 Size: 245 1459 EarI CTCTTCn'nnn_ Cuts at: 0 904 1704 Size: 904 800 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1297 1704 Size: 1297 407 EcoICRI GAG'CTC Cuts at: 0 228 1704 Size: 228 1476 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 634 820 1318 1704 Size: 634 186 498 386 Fragments arranged by size: 634 498 386 186 EcoNI CCTnn'n_nnAGG Cuts at: 0 1120 1246 1642 1704 Size: 1120 126 396 62 Fragments arranged by size: 1120 396 126 62 EcoO109I rG'GnC_Cy Cuts at: 0 138 235 459 759 1214 1557 1704 Size: 138 97 224 300 455 343 147 Fragments arranged by size: 455 343 300 224 147 138 97 EcoRI G'AATT_C Cuts at: 0 573 1704 Size: 573 1131 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 849 1704 Size: 849 855 HaeII r_GCGC'y Cuts at: 0 541 739 1638 1658 1704 Size: 541 198 899 20 46 Fragments arranged by size: 899 541 198 46 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1 33 241 273 274 306 958 990 Size: 1 32 208 32 1 32 652 32 Cuts at: 990 1327 1359 1372 1404 1704 Size: 337 32 13 32 300 Fragments arranged by size: 652 337 300 208 32 32 32 32 32 32 13 1 1 HincII GTy'rAC Cuts at: 0 1131 1257 1470 1704 Size: 1131 126 213 234 Fragments arranged by size: 1131 234 213 126 KasI G'GCGC_C Cuts at: 0 537 735 1704 Size: 537 198 969 Fragments arranged by size: 969 537 198 KpnI G_GTAC'C Cuts at: 0 1511 1704 Size: 1511 193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1370 1704 Size: 1370 334 MscI TGG'CCA Cuts at: 0 115 1663 1704 Size: 115 1548 41 Fragments arranged by size: 1548 115 41 MslI CAynn'nnrTG Cuts at: 0 850 1357 1704 Size: 850 507 347 Fragments arranged by size: 850 507 347 MspA1I CmG'CkG Cuts at: 0 600 686 1323 1704 Size: 600 86 637 381 Fragments arranged by size: 637 600 381 86 NarI GG'CG_CC Cuts at: 0 538 736 1704 Size: 538 198 968 Fragments arranged by size: 968 538 198 NcoI C'CATG_G Cuts at: 0 1658 1704 Size: 1658 46 NspI r_CATG'y Cuts at: 0 1581 1704 Size: 1581 123 PflMI CCAn_nnn'nTGG Cuts at: 0 141 567 1704 Size: 141 426 1137 Fragments arranged by size: 1137 426 141 PfoI T'CCnGG_A Cuts at: 0 134 797 1154 1418 1704 Size: 134 663 357 264 286 Fragments arranged by size: 663 357 286 264 134 PpuMI rG'GwC_Cy Cuts at: 0 138 459 1557 1704 Size: 138 321 1098 147 Fragments arranged by size: 1098 321 147 138 PspOMI G'GGCC_C Cuts at: 0 234 1704 Size: 234 1470 PstI C_TGCA'G Cuts at: 0 605 1121 1704 Size: 605 516 583 Fragments arranged by size: 605 583 516 PvuII CAG'CTG Cuts at: 0 600 686 1704 Size: 600 86 1018 Fragments arranged by size: 1018 600 86 SacI G_AGCT'C Cuts at: 0 230 1704 Size: 230 1474 SbfI CC_TGCA'GG Cuts at: 0 1121 1704 Size: 1121 583 SexAI A'CCwGG_T Cuts at: 0 1463 1704 Size: 1463 241 SfcI C'TryA_G Cuts at: 0 601 1117 1704 Size: 601 516 587 Fragments arranged by size: 601 587 516 SfoI GGC'GCC Cuts at: 0 539 737 1704 Size: 539 198 967 Fragments arranged by size: 967 539 198 SmlI C'TyrA_G Cuts at: 0 340 727 1704 Size: 340 387 977 Fragments arranged by size: 977 387 340 StyI C'CwwG_G Cuts at: 0 203 239 455 566 1277 1485 1658 1704 Size: 203 36 216 111 711 208 173 46 Fragments arranged by size: 711 216 208 203 173 111 46 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 75 139 1704 Size: 75 64 1565 Fragments arranged by size: 1565 75 64 Enzymes that do cut: AarI Acc65I AflIII AhdI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I NarI NcoI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SbfI SexAI SfcI SfoI SmlI StyI TaqII Enzymes that do not cut: AatII AccI AclI AcuI AfeI AflII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BlpI BmrI BmtI BplI BsaI BsaXI BsiWI BsmI BsmBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI EcoRV FseI FspI FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI