##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AviII       BalI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeII       MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
StuI        SwaI        Tth111I     Van91I      XbaI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   20 enzymes) #
###################################

AccI         1262  GTCTAC               GT^[AC][GT]AC
Asp700I       731  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        1408  CCTAGG               C^CTAGG
BglII         954  AGATCT               A^GATCT
BlnI         1408  CCTAGG               C^CTAGG
BsaBI         387  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI        1310  GAGAC                GTCTC(1/5)
BspMI         281  ACCTGC               ACCTGC(4/8)
BstEII        456  GGTGACC              G^GT[ACGT]ACC
BstPI         456  GGTGACC              G^GT[ACGT]ACC
EcoO65I       456  GGTGACC              G^GT[ACGT]ACC
EcoRI         377  GAATTC               G^AATTC
MamI          387  GATAGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          950  TTAA                 T^TAA
PpuMI        1196  AGGTCCT              [GA]G^G[AT]CC[CT]
SmlI          540  CTCAAG               C^T[CT][GA]AG
SspI         1057  AATATT               AAT^ATT
Tru9I         950  TTAA                 T^TAA
Tsp45I        457  GTGAC                ^GT[GC]AC
XmnI          731  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcsI            4  AAATTC               [GA]^AATT[CT]
              377  GAATTC              
AfaI          273  GTAC                 GT^AC
              853  GTAC                
AlwNI         791  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
             1195  CAGGTCCTG           
ApoI            4  AAATTC               [GA]^AATT[CT]
              377  GAATTC              
AspHI          51  GTGCTC               G[AT]GC[AT]^C
              991  GTGCTC              
BamHI        1235  GGATCC               G^GATCC
             1329  GGATCC              
BanI          895  GGTGCC               G^G[CT][GA]CC
             1024  GGTGCC              
BseRI         422  CTCCTC               GAGGAG(10/8)
             1156  GAGGAG              
BsiHKAI        51  GTGCTC               G[AT]GC[AT]^C
              991  GTGCTC              
BsmFI         418  GGGAC                GGGAC(10/14)
             1216  GGGAC               
DraII         484  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1196  AGGTCCT             
EcoNI         424  CCTCCAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              643  CCTCACACAGG         
EcoO109I      484  AGGGCCT              [GA]G^G[ACGT]CC[CT]
             1196  AGGTCCT             
HgaI         1090  GACGC                GACGC(5/10)
              966  GCGTC               
MaeIII        457  GTGAC                ^GT[ACGT]AC
             1044  GTTAC               
NcoI          899  CCATGG               C^CATGG
             1206  CCATGG              
RsaI          273  GTAC                 GT^AC
              853  GTAC                
TaqI          467  TCGA                 T^CGA
              612  TCGA                
TthHB8I       467  TCGA                 T^CGA
              612  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MboI        MboII       MflI        MnlI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TfiI        TseI        Tsp509I     TspRI       XcmI        
XhoII