(Circular) (Six-base) MAPSORT of: pFN21AB5061.seq Check: 3230 from: 1 to: 6121 pFN21AB5061 6121 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3268 3464 1937 Size: 1331 196 4594 Fragments arranged by size: 4594 1331 196 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4184 5568 5836 1632 Size: 2552 1384 268 1917 Fragments arranged by size: 2552 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 4952 4952 Size: 6121 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6121 AlwNI CAG_nnn'CTG Cuts at: 2801 3205 3864 5436 2801 Size: 404 659 1572 3486 Fragments arranged by size: 3486 1572 659 404 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2009 2382 3430 3656 3808 5917 1493 Size: 516 373 1048 226 152 2109 1697 Fragments arranged by size: 2109 1697 1048 516 373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 AvrII C'CTAG_G Cuts at: 3413 3413 Size: 6121 BamHI G'GATC_C Cuts at: 3240 3334 3451 3818 3240 Size: 94 117 367 5543 Fragments arranged by size: 5543 367 117 94 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2900 3029 3442 4897 618 Size: 325 217 627 1113 129 413 1455 1842 Fragments arranged by size: 1842 1455 1113 627 413 325 217 129 BanII G_rGCy'C Cuts at: 1391 3440 1391 Size: 2049 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 136 Size: 107 122 71 631 605 3060 1525 Fragments arranged by size: 3060 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2959 6116 2959 Size: 3157 2964 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 2685 4182 5338 1790 Size: 895 1497 1156 2573 Fragments arranged by size: 2573 1497 1156 895 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2350 2841 3126 Size: 416 53 83 399 24 933 491 285 Cuts at: 3126 3852 4810 442 Size: 726 958 1753 Fragments arranged by size: 1753 958 933 726 491 416 399 285 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2339 2393 2415 3270 3279 4781 1239 Size: 1085 15 54 22 855 9 1502 2579 Fragments arranged by size: 2579 1502 1085 855 54 22 15 9 Bpu10I CC'TnA_GC Cuts at: 3206 3206 Size: 6121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2530 4246 5111 5409 2530 Size: 1716 865 298 3242 Fragments arranged by size: 3242 1716 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 2396 6115 2396 Size: 3719 2402 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4554 4952 5226 5373 5784 4554 Size: 398 274 147 411 4891 Fragments arranged by size: 4891 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5896 Fragments arranged by size: 5896 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2418 3176 1186 Size: 1232 758 4131 Fragments arranged by size: 4131 1232 758 BseYI C'CCAG_C Cuts at: 2883 5324 5820 2883 Size: 2441 496 3184 Fragments arranged by size: 3184 2441 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2060 3000 4182 4267 5338 2060 Size: 940 1182 85 1071 2843 Fragments arranged by size: 2843 1182 1071 940 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2060 2685 3000 3440 4182 4267 5338 Size: 399 270 625 315 440 742 85 1071 Cuts at: 5338 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 625 440 399 315 270 85 BspEI T'CCGG_A Cuts at: 5784 5784 Size: 6121 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 3476 844 Size: 1451 1181 3489 Fragments arranged by size: 3489 1451 1181 BsrBI CCG'CTC Cuts at: 1394 1823 4008 1394 Size: 429 2185 3507 Fragments arranged by size: 3507 2185 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4765 4952 1700 Size: 110 2955 187 2869 Fragments arranged by size: 2955 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6121 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 2461 1184 Size: 1277 4844 BstYI r'GATC_y Cuts at: 1972 2189 2959 3240 3334 3451 3818 4203 4220 Size: 217 770 281 94 117 367 385 17 Cuts at: 4220 5672 5758 5770 6116 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 770 385 367 346 281 217 117 94 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2622 2904 3211 3310 513 Size: 552 1557 282 307 99 3324 Fragments arranged by size: 3324 1557 552 307 282 99 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5382 Fragments arranged by size: 5382 683 56 BtsI GCAGTG_nn' Cuts at: 3142 3637 4431 4451 3142 Size: 495 794 20 4812 Fragments arranged by size: 4812 794 495 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3568 4457 6086 Size: 54 1425 50 275 18 1738 889 1629 Cuts at: 6086 8 Size: 43 Fragments arranged by size: 1738 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4051 1353 Size: 2698 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4708 5082 5228 387 Size: 1064 138 3119 374 146 1280 Fragments arranged by size: 3119 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2339 2393 2415 3270 3279 4184 Size: 393 692 15 54 22 855 9 905 Cuts at: 4184 4781 5568 5836 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 905 855 787 692 597 393 268 54 22 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 2433 2652 2433 Size: 219 5902 EcoO109I rG'GnC_Cy Cuts at: 2490 3202 2490 Size: 712 5409 EcoRI G'AATT_C Cuts at: 2382 3430 3808 2382 Size: 1048 378 4695 Fragments arranged by size: 4695 1048 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2757 3310 2757 Size: 553 5568 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 5268 1678 Size: 3590 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2827 2859 4762 4794 4836 Size: 32 135 32 1255 32 1903 32 42 Cuts at: 4836 4868 1373 Size: 32 2626 Fragments arranged by size: 2626 1903 1255 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3465 3711 669 Size: 988 281 1527 246 3079 Fragments arranged by size: 3079 1527 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 3711 3711 Size: 6121 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2206 2717 3093 5235 5419 1305 Size: 901 511 376 2142 184 2007 Fragments arranged by size: 2142 2007 901 511 376 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4299 Fragments arranged by size: 4299 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4079 4438 4597 518 Size: 648 2913 359 159 2042 Fragments arranged by size: 2913 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2130 2363 2883 3537 4214 5362 5607 1606 Size: 524 233 520 654 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 654 524 520 245 233 NcoI C'CATG_G Cuts at: 513 1065 2904 3211 513 Size: 552 1839 307 3423 Fragments arranged by size: 3423 1839 552 307 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 3479 5024 1132 Size: 97 2250 1545 2229 Fragments arranged by size: 2250 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6121 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3202 3202 Size: 6121 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6121 PsiI TTA'TAA Cuts at: 2523 3691 2523 Size: 1168 4953 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2841 2873 2841 Size: 32 6089 PstI C_TGCA'G Cuts at: 830 2292 2484 3010 3183 3473 830 Size: 1462 192 526 173 290 3478 Fragments arranged by size: 3478 1462 526 290 192 173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2130 2363 2883 2130 Size: 233 520 5368 Fragments arranged by size: 5368 520 233 SalI G'TCGA_C Cuts at: 1936 3463 1936 Size: 1527 4594 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2288 2480 2641 2817 3006 3073 3179 Size: 219 1243 192 161 176 189 67 106 Cuts at: 3179 3469 4604 5285 5476 826 Size: 290 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 681 290 219 192 191 189 176 161 106 67 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2545 4225 5126 5388 820 Size: 197 926 602 1680 901 262 1553 Fragments arranged by size: 1680 1553 926 901 602 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 3479 1132 Size: 2347 3774 SspI AAT'ATT Cuts at: 5 52 3064 4045 5 Size: 47 3012 981 2081 Fragments arranged by size: 3012 2081 981 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2589 2904 3211 3413 3560 513 Size: 552 1524 315 307 202 147 3074 Fragments arranged by size: 3074 1524 552 315 307 202 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4148 4165 4318 4503 4948 325 Size: 1255 2568 17 153 185 445 1498 Fragments arranged by size: 2568 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2857 4367 6079 Size: 274 80 33 51 1443 880 1510 1712 Cuts at: 6079 96 Size: 138 Fragments arranged by size: 1712 1510 1443 880 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2033 2065 6081 6113 2033 Size: 32 4016 32 2041 Fragments arranged by size: 4016 2041 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3076 3154 3328 3076 Size: 78 174 5869 Fragments arranged by size: 5869 174 78 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 2740 4250 2740 Size: 1510 4611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI