Restriction map for
pFN21AB5061
(Linear) (Six-base) MAPSORT of: KIBB5061.seq Check: 421 from: 1 to: 1416
KIBB5061.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 0 1263 1416
Size: 1263 153
AlwNI CAG_nnn'CTG
Cuts at: 0 796 1200 1416
Size: 796 404 216
Fragments arranged by size:
796 404 216
ApoI r'AATT_y
Cuts at: 0 4 377 1416
Size: 4 373 1039
Fragments arranged by size:
1039 373 4
AvrII C'CTAG_G
Cuts at: 0 1408 1416
Size: 1408 8
BamHI G'GATC_C
Cuts at: 0 1235 1329 1416
Size: 1235 94 87
Fragments arranged by size:
1235 94 87
BanI G'GyrC_C
Cuts at: 0 895 1024 1416
Size: 895 129 392
Fragments arranged by size:
895 392 129
BglII A'GATC_T
Cuts at: 0 954 1416
Size: 954 462
Bme1580I G_kGCm'C
Cuts at: 0 680 1416
Size: 680 736
BmrI ACTGGGnnnn_n'
Cuts at: 0 345 836 1121 1416
Size: 345 491 285 295
Fragments arranged by size:
491 345 295 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 334 388 410 1265 1274 1416
Size: 319 15 54 22 855 9 142
Fragments arranged by size:
855 319 142 54 22 15 9
Bpu10I CC'TnA_GC
Cuts at: 0 1201 1416
Size: 1201 215
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1416
Size: 525 891
BsaBI GATnn'nnATC
Cuts at: 0 391 1416
Size: 391 1025
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 413 1171 1416
Size: 413 758 245
Fragments arranged by size:
758 413 245
BseYI C'CCAG_C
Cuts at: 0 878 1416
Size: 878 538
BsiHKAI G_wGCw'C
Cuts at: 0 55 995 1416
Size: 55 940 421
Fragments arranged by size:
940 421 55
Bsp1286I G_dGCh'C
Cuts at: 0 55 680 995 1416
Size: 55 625 315 421
Fragments arranged by size:
625 421 315 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 290 1416
Size: 290 1126
BstEII G'GTnAC_C
Cuts at: 0 456 1416
Size: 456 960
BstYI r'GATC_y
Cuts at: 0 184 954 1235 1329 1416
Size: 184 770 281 94 87
Fragments arranged by size:
770 281 184 94 87
BtgI C'CryG_G
Cuts at: 0 617 899 1206 1305 1416
Size: 617 282 307 99 111
Fragments arranged by size:
617 307 282 111 99
BtsI GCAGTG_nn'
Cuts at: 0 1137 1416
Size: 1137 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 334 388 410 1265 1274 1416
Size: 319 15 54 22 855 9 142
Fragments arranged by size:
855 319 142 54 22 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 428 647 1416
Size: 428 219 769
Fragments arranged by size:
769 428 219
EcoO109I rG'GnC_Cy
Cuts at: 0 485 1197 1416
Size: 485 712 219
Fragments arranged by size:
712 485 219
EcoRI G'AATT_C
Cuts at: 0 377 1416
Size: 377 1039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 752 1305 1416
Size: 752 553 111
Fragments arranged by size:
752 553 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 822 854 1416
Size: 822 32 562
Fragments arranged by size:
822 562 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 712 1088 1416
Size: 201 511 376 328
Fragments arranged by size:
511 376 328 201
MspA1I CmG'CkG
Cuts at: 0 125 358 878 1416
Size: 125 233 520 538
Fragments arranged by size:
538 520 233 125
NcoI C'CATG_G
Cuts at: 0 899 1206 1416
Size: 899 307 210
Fragments arranged by size:
899 307 210
PpuMI rG'GwC_Cy
Cuts at: 0 1197 1416
Size: 1197 219
PsiI TTA'TAA
Cuts at: 0 518 1416
Size: 518 898
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 836 868 1416
Size: 836 32 548
Fragments arranged by size:
836 548 32
PstI C_TGCA'G
Cuts at: 0 287 479 1005 1178 1416
Size: 287 192 526 173 238
Fragments arranged by size:
526 287 238 192 173
PvuII CAG'CTG
Cuts at: 0 125 358 878 1416
Size: 125 233 520 538
Fragments arranged by size:
538 520 233 125
SfcI C'TryA_G
Cuts at: 0 283 475 636 812 1001 1068 1174 1416
Size: 283 192 161 176 189 67 106 242
Fragments arranged by size:
283 242 192 189 176 161 106 67
SmlI C'TyrA_G
Cuts at: 0 540 1416
Size: 540 876
SspI AAT'ATT
Cuts at: 0 1059 1416
Size: 1059 357
StyI C'CwwG_G
Cuts at: 0 584 899 1206 1408 1416
Size: 584 315 307 202 8
Fragments arranged by size:
584 315 307 202 8
TatI w'GTAC_w
Cuts at: 0 852 1416
Size: 852 564
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 1416
Size: 28 32 1356
Fragments arranged by size:
1356 32 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1149 1323 1416
Size: 1071 78 174 93
Fragments arranged by size:
1071 174 93 78
XmnI GAAnn'nnTTC
Cuts at: 0 735 1416
Size: 735 681
Enzymes that do cut:
AccI AlwNI ApoI AvrII BamHI BanI BglII Bme1580I
BmrI BpmI Bpu10I BpuEI BsaBI BseRI BseYI BsiHKAI
Bsp1286I BspMI BstEII BstYI BtgI BtsI Eco57MI EcoNI
EcoO109I EcoRI FalI Hin4I MmeI MspA1I NcoI PpuMI
PsiI PsrI PstI PvuII SfcI SmlI SspI StyI
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI BaeI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI BmgBI BmtI
BplI BsaI BsaAI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI