################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I FbaI FseI FspI HindIII HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 729 CGCG CG^CG AcsI 184 GAATTC [GA]^AATT[CT] AcyI 14 GACGCC G[GA]^CG[CT]C AfaI 855 GTAC GT^AC AlwNI 712 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 631 GGGCCC GGGCC^C ApoI 184 GAATTC [GA]^AATT[CT] AvaI 18 CCCGGG C^[CT]CG[GA]G BanI 3 GGCACC G^G[CT][GA]CC BbsI 648 GAAGAC GAAGAC(2/6) BglI 826 GCCAGAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 648 GAAGAC GAAGAC(2/6) BsaHI 14 GACGCC G[GA]^CG[CT]C BseRI 907 GAGGAG GAGGAG(10/8) BsgI 709 GTGCAG GTGCAG(16/14) BsmAI 545 GAGAC GTCTC(1/5) BsoBI 18 CCCGGG C^[CT]CG[GA]G BspHI 662 TCATGA T^CATGA BspMI 6 ACCTGC ACCTGC(4/8) BstF5I 369 GGATG GGATG(2/0) BstUI 729 CGCG CG^CG BstXI 293 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 1055 GACCTTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 512 CCGTGG C^C[GA][CT]GG EarI 102 GAAGAG CTCTTC(1/4) EcoRI 184 GAATTC G^AATTC EcoT22I 550 ATGCAT ATGCA^T FauI 863 GCGGG CCCGC(4/6) FokI 369 GGATG GGATG(9/13) HaeII 44 AGCGCC [GA]GCGC^[CT] Hin1I 14 GACGCC G[GA]^CG[CT]C HincII 170 GTCAAC GT[CT]^[GA]AC HindII 170 GTCAAC GT[CT]^[GA]AC Ksp632I 102 GAAGAG CTCTTC(1/4) MaeII 461 ACGT A^CGT MfeI 899 CAATTG C^AATTG MspA1I 960 CAGCTG C[AC]G^C[GT]G MunI 899 CAATTG C^AATTG MvnI 729 CGCG CG^CG NsiI 550 ATGCAT ATGCA^T PshAI 782 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 960 CAGCTG CAG^CTG RcaI 662 TCATGA T^CATGA RsaI 855 GTAC GT^AC SacI 658 GAGCTC GAGCT^C SfaNI 316 GATGC GCATC(5/9) SmaI 18 CCCGGG CCC^GGG StuI 833 AGGCCT AGG^CCT Tsp45I 284 GTGAC ^GT[GC]AC XmaCI 18 CCCGGG C^CCGGG XmaI 18 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 624 CCGC CCGC(-3/-1) 863 GCGG AspHI 457 GAGCAC G[AT]GC[AT]^C 658 GAGCTC BfaI 377 CTAG C^TAG 406 CTAG BsiHKAI 457 GAGCAC G[AT]GC[AT]^C 658 GAGCTC BsrI 783 ACTGG ACTGG(1/-1) 233 CCAGT BstYI 335 GGATCT [GA]^GATC[CT] 759 GGATCT HapII 19 CCGG C^CGG 566 CCGG HgaI 14 GACGC GACGC(5/10) 160 GACGC HpaII 19 CCGG C^CGG 566 CCGG HphI 313 GGTGA GGTGA(8/7) 588 GGTGA MaeI 377 CTAG C^TAG 406 CTAG MaeIII 284 GTGAC ^GT[ACGT]AC 520 GTTAC MflI 335 GGATCT [GA]^GATC[CT] 759 GGATCT MseI 383 TTAA T^TAA 493 TTAA MspI 19 CCGG C^CGG 566 CCGG NspI 548 ACATGC [GA]CATG^[CT] 980 ACATGC PflMI 800 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 989 CCAAACCCTGG PleI 132 GACTC GAGTC(4/5) 787 GAGTC SmlI 189 CTTGAG C^T[CT][GA]AG 676 CTTGAG TaqI 396 TCGA T^CGA 1014 TCGA TfiI 499 GAATC G^A[AT]TC 1032 GAATC Tru9I 383 TTAA T^TAA 493 TTAA TspRI 143 CAGTG CA[GC]TG[ACGT][ACGT]^ 234 CAGTG TthHB8I 396 TCGA T^CGA 1014 TCGA Van91I 800 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 989 CCAAACCCTGG XhoII 335 GGATCT [GA]^GATC[CT] 759 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AluI AlwI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboI MboII MnlI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TseI Tsp509I