################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NarI NdeI NdeII NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SfcI SfiI SfoI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccI 616 GTAGAC GT^[AC][GT]AC AccII 223 CGCG CG^CG AflIII 94 ACATGT A^C[GA][CT]GT BcgI 843 CGACTGTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsmBI 308 GAGACG CGTCTC(1/5) BspLU11I 94 ACATGT A^CATGT BsrBI 384 GAGCGG CCGCTC(-3/-3) BsrFI 416 GCCGGC [GA]^CCGG[CT] BstBI 801 TTCGAA TT^CGAA BstUI 223 CGCG CG^CG Cfr10I 416 GCCGGC [GA]^CCGG[CT] DsaI 20 CCATGG C^C[GA][CT]GG EcoT14I 20 CCATGG C^C[AT][AT]GG HgaI 310 GACGC GACGC(5/10) MaeIII 437 GTGAC ^GT[ACGT]AC MvnI 223 CGCG CG^CG NaeI 416 GCCGGC GCC^GGC NcoI 20 CCATGG C^CATGG NgoMIV 416 GCCGGC G^CCGGC NspI 94 ACATGT [GA]CATG^[CT] NspV 801 TTCGAA TT^CGAA PshAI 91 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 544 CAGCTG CAG^CTG SacI 607 GAGCTC GAGCT^C SexAI 620 ACCTGGT A^CC[AT]GGT SfaNI 291 GATGC GCATC(5/9) SfuI 801 TTCGAA TT^CGAA StyI 20 CCATGG C^C[AT][AT]GG TaqI 802 TCGA T^CGA Tsp45I 437 GTGAC ^GT[GC]AC TthHB8I 802 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### ApaI 173 GGGCCC GGGCC^C 262 GGGCCC AspHI 430 GTGCTC G[AT]GC[AT]^C 607 GAGCTC BfaI 46 CTAG C^TAG 364 CTAG BglI 16 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 119 GCCTGGAGGGC BseRI 215 GAGGAG GAGGAG(10/8) 278 GAGGAG BsiHKAI 430 GTGCTC G[AT]GC[AT]^C 607 GAGCTC BsmFI 509 GGGAC GGGAC(10/14) 882 GGGAC FauI 973 CCCGC CCCGC(4/6) 730 GCGGG HphI 105 GGTGA GGTGA(8/7) 140 TCACC MaeI 46 CTAG C^TAG 364 CTAG MboII 764 TCTTC GAAGA(8/7) 799 TCTTC MspA1I 475 CAGCGG C[AC]G^C[GT]G 544 CAGCTG PpuMI 356 AGGACCT [GA]G^G[AT]CC[CT] 882 GGGACCC Tsp509I 736 AATT ^AATT 805 AATT TspRI 257 CAGTG CA[GC]TG[ACGT][ACGT]^ 726 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI SmaI TseI XmaCI XmaI