(Circular) (Six-base) MAPSORT of: pFN21AE4324.seq Check: 4280 from: 1 to: 5683 pFN21AE4324 5683 bp With 182 enzymes: SgfI * July 6, 2012 17:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2192 2192 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2622 3026 1937 Size: 685 404 4594 Fragments arranged by size: 4594 685 404 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3746 5130 5398 1632 Size: 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2099 4582 5559 1203 Size: 896 2483 977 1327 Fragments arranged by size: 2483 1327 977 896 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2381 1184 Size: 1197 4486 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2217 2249 2217 Size: 32 5651 AlwNI CAG_nnn'CTG Cuts at: 3426 4998 3426 Size: 1572 4111 ApaI G_GGCC'C Cuts at: 2182 2271 2182 Size: 89 5594 ApaLI G'TGCA_C Cuts at: 3740 4896 3740 Size: 1156 4527 ApoI r'AATT_y Cuts at: 1493 2992 3218 3370 5479 1493 Size: 1499 226 152 2109 1697 Fragments arranged by size: 2109 1697 1499 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2031 2164 2174 3008 1870 Size: 73 88 133 10 834 4545 Fragments arranged by size: 4545 834 133 88 73 10 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3004 4459 618 Size: 325 217 627 1217 1455 1842 Fragments arranged by size: 1842 1455 1217 627 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2182 2245 2271 2616 3002 1391 Size: 744 47 63 26 345 386 4072 Fragments arranged by size: 4072 744 386 345 63 47 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4712 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2837 2871 3874 3908 1903 Size: 34 900 34 1003 34 3678 Fragments arranged by size: 3678 1003 900 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2027 2130 4294 Size: 107 122 71 631 605 355 103 2164 Cuts at: 4294 136 Size: 1525 Fragments arranged by size: 2164 1525 631 605 355 122 107 103 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 3100 3100 Size: 5683 Bme1580I G_kGCm'C Cuts at: 1790 2182 2271 3744 4900 1790 Size: 392 89 1473 1156 2573 Fragments arranged by size: 2573 1473 1156 392 89 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5683 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 2720 3414 Size: 416 53 83 399 24 1110 193 694 Cuts at: 3414 4372 442 Size: 958 1753 Fragments arranged by size: 1753 1110 958 694 416 399 193 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2259 2941 4343 1239 Size: 908 112 682 1402 2579 Fragments arranged by size: 2579 1402 908 682 112 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 5683 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2042 2237 2271 2879 4346 882 Size: 617 543 195 34 608 1467 2219 Fragments arranged by size: 2219 1467 617 608 543 195 34 BsaAI yAC'GTr Cuts at: 493 1204 3474 493 Size: 711 2270 2702 Fragments arranged by size: 2702 2270 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3490 3872 4507 275 Size: 53 83 186 936 1957 382 635 1451 Fragments arranged by size: 1957 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2217 2247 2706 2736 2756 Size: 30 165 30 814 30 459 30 20 Cuts at: 2756 2786 1178 Size: 30 4075 Fragments arranged by size: 4075 814 459 165 30 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2235 2298 1186 Size: 1049 63 4571 Fragments arranged by size: 4571 1049 63 BseYI C'CCAG_C Cuts at: 2318 2551 2566 4886 5382 2318 Size: 233 15 2320 496 2619 Fragments arranged by size: 2619 2320 496 233 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5110 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2439 2616 3744 3829 4900 2439 Size: 177 1128 85 1071 3222 Fragments arranged by size: 3222 1128 1071 177 85 BsmI GAATG_Cn' Cuts at: 3194 3287 3194 Size: 93 5590 BsmBI CGTCTCn'nnnn_ Cuts at: 2307 2307 Size: 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2182 2245 2271 2439 2616 3002 Size: 399 345 47 63 26 168 177 386 Cuts at: 3002 3744 3829 4900 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 386 345 177 168 85 63 47 26 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2192 2373 2418 3038 844 Size: 1241 107 181 45 620 3489 Fragments arranged by size: 3489 1241 620 181 107 45 BsrBI CCG'CTC Cuts at: 1394 1823 2391 3570 1394 Size: 429 568 1179 3507 Fragments arranged by size: 3507 1179 568 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2421 4327 4514 1700 Size: 110 611 1906 187 2869 Fragments arranged by size: 2869 1906 611 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5683 BstBI TT'CG_AA Cuts at: 2807 4487 2807 Size: 1680 4003 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 513 Size: 552 939 21 4171 Fragments arranged by size: 4171 939 552 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2737 3199 3993 4013 2737 Size: 462 794 20 4407 Fragments arranged by size: 4407 794 462 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3130 4019 5648 Size: 54 1425 50 275 18 1300 889 1629 Cuts at: 5648 8 Size: 43 Fragments arranged by size: 1629 1425 1300 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3613 1353 Size: 2260 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2614 2614 Size: 5683 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2259 2941 3746 4343 5130 5398 Size: 393 515 112 682 805 597 787 268 Cuts at: 5398 1239 Size: 1524 Fragments arranged by size: 1524 805 787 682 597 515 393 268 112 EcoO109I rG'GnC_Cy Cuts at: 2178 2179 2267 2362 2888 2178 Size: 1 88 95 526 4973 Fragments arranged by size: 4973 526 95 88 1 EcoRI G'AATT_C Cuts at: 2992 3370 2992 Size: 378 5305 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2554 2554 Size: 5683 FspI TGC'GCA Cuts at: 4189 4189 Size: 5683 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2706 2738 4324 4356 4398 Size: 32 135 32 1134 32 1586 32 42 Cuts at: 4398 4430 1373 Size: 32 2626 Fragments arranged by size: 2626 1586 1134 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 3282 3282 Size: 5683 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4797 4981 1305 Size: 3492 184 2007 Fragments arranged by size: 3492 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3861 Fragments arranged by size: 3861 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2482 2551 3099 3776 4924 5169 1606 Size: 876 69 548 677 1148 245 2120 Fragments arranged by size: 2120 1148 876 677 548 245 69 NaeI GCC'GGC Cuts at: 2423 2423 Size: 5683 NcoI C'CATG_G Cuts at: 513 1065 2004 2025 513 Size: 552 939 21 4171 Fragments arranged by size: 4171 939 552 21 NdeI CA'TA_TG Cuts at: 387 387 Size: 5683 NgoMIV G'CCGG_C Cuts at: 2421 2421 Size: 5683 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2103 3041 4586 1132 Size: 97 874 938 1545 2229 Fragments arranged by size: 2229 1545 938 874 97 PasI CC'CwG_GG Cuts at: 1348 2183 2599 1348 Size: 835 416 4432 Fragments arranged by size: 4432 835 416 PciI A'CATG_T Cuts at: 2099 4582 2099 Size: 2483 3200 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2217 2249 3752 3784 5265 5297 2217 Size: 32 1503 32 1481 32 2603 Fragments arranged by size: 2603 1503 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2362 2888 2362 Size: 526 5157 PshAI GACnn'nnGTC Cuts at: 1613 2100 1613 Size: 487 5196 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2178 2267 2178 Size: 89 5594 PstI C_TGCA'G Cuts at: 830 3035 830 Size: 2205 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2551 2551 Size: 5683 SacI G_AGCT'C Cuts at: 2616 2616 Size: 5683 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SanDI GG'GwC_CC Cuts at: 2888 2888 Size: 5683 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 3931 5643 3931 Size: 1712 3971 SexAI A'CCwGG_T Cuts at: 2625 2625 Size: 5683 SfcI C'TryA_G Cuts at: 826 1045 3031 4166 4847 5038 826 Size: 219 1986 1135 681 191 1471 Fragments arranged by size: 1986 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2033 2166 2176 3010 1872 Size: 161 133 10 834 4545 Fragments arranged by size: 4545 834 161 133 10 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3474 493 Size: 2981 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5683 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5683 StyI C'CwwG_G Cuts at: 513 1065 2004 2025 3122 513 Size: 552 939 21 1097 3074 Fragments arranged by size: 3074 1097 939 552 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2295 3710 3727 3880 4065 4510 325 Size: 1255 715 1415 17 153 185 445 1498 Fragments arranged by size: 1498 1415 1255 715 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2661 2697 3929 Size: 274 80 33 51 1443 684 36 1232 Cuts at: 3929 5641 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1232 684 274 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5643 5675 5643 Size: 32 5651 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2031 2164 2174 3008 1870 Size: 161 133 10 834 4545 Fragments arranged by size: 4545 834 161 133 10 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI