(Linear) (Six-base) MAPSORT of: KIEE4324.seq Check: 5746 from: 1 to: 978 KIEE4324.orf With 182 enzymes: SgfI * July 3, 2012 18:58 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 187 978 Size: 187 791 AccI GT'mk_AC Cuts at: 0 617 978 Size: 617 361 AflIII A'CryG_T Cuts at: 0 94 978 Size: 94 884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 376 978 Size: 376 602 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 212 244 978 Size: 212 32 734 Fragments arranged by size: 734 212 32 ApaI G_GGCC'C Cuts at: 0 177 266 978 Size: 177 89 712 Fragments arranged by size: 712 177 89 AvaI C'yCGr_G Cuts at: 0 26 159 169 978 Size: 26 133 10 809 Fragments arranged by size: 809 133 26 10 BanII G_rGCy'C Cuts at: 0 130 177 240 266 611 978 Size: 130 47 63 26 345 367 Fragments arranged by size: 367 345 130 63 47 26 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 832 866 978 Size: 832 34 112 Fragments arranged by size: 832 112 34 BglI GCCn_nnn'nGGC Cuts at: 0 22 125 978 Size: 22 103 853 Fragments arranged by size: 853 103 22 Bme1580I G_kGCm'C Cuts at: 0 177 266 978 Size: 177 89 712 Fragments arranged by size: 712 177 89 BmrI ACTGGGnnnn_n' Cuts at: 0 522 715 978 Size: 522 193 263 Fragments arranged by size: 522 263 193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 254 936 978 Size: 142 112 682 42 Fragments arranged by size: 682 142 112 42 Bpu10I CC'TnA_GC Cuts at: 0 382 978 Size: 382 596 BsaI GGTCTCn'nnnn_ Cuts at: 0 37 232 266 874 978 Size: 37 195 34 608 104 Fragments arranged by size: 608 195 104 37 34 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 212 242 701 731 751 781 978 Size: 212 30 459 30 20 30 197 Fragments arranged by size: 459 212 197 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 230 293 978 Size: 230 63 685 Fragments arranged by size: 685 230 63 BseYI C'CCAG_C Cuts at: 0 313 546 561 978 Size: 313 233 15 417 Fragments arranged by size: 417 313 233 15 BsiHKAI G_wGCw'C Cuts at: 0 434 611 978 Size: 434 177 367 Fragments arranged by size: 434 367 177 BsmBI CGTCTCn'nnnn_ Cuts at: 0 302 978 Size: 302 676 Bsp1286I G_dGCh'C Cuts at: 0 130 177 240 266 434 611 978 Size: 130 47 63 26 168 177 367 Fragments arranged by size: 367 177 168 130 63 47 26 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 80 187 368 413 978 Size: 80 107 181 45 565 Fragments arranged by size: 565 181 107 80 45 BsrBI CCG'CTC Cuts at: 0 386 978 Size: 386 592 BsrFI r'CCGG_y Cuts at: 0 416 978 Size: 416 562 BstBI TT'CG_AA Cuts at: 0 802 978 Size: 802 176 BtgI C'CryG_G Cuts at: 0 20 978 Size: 20 958 BtsI GCAGTG_nn' Cuts at: 0 732 978 Size: 732 246 EcoICRI GAG'CTC Cuts at: 0 609 978 Size: 609 369 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 254 936 978 Size: 142 112 682 42 Fragments arranged by size: 682 142 112 42 EcoO109I rG'GnC_Cy Cuts at: 0 173 174 262 357 883 978 Size: 173 1 88 95 526 95 Fragments arranged by size: 526 173 95 95 88 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 549 978 Size: 549 429 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 701 733 978 Size: 701 32 245 Fragments arranged by size: 701 245 32 MspA1I CmG'CkG Cuts at: 0 477 546 978 Size: 477 69 432 Fragments arranged by size: 477 432 69 NaeI GCC'GGC Cuts at: 0 418 978 Size: 418 560 NcoI C'CATG_G Cuts at: 0 20 978 Size: 20 958 NgoMIV G'CCGG_C Cuts at: 0 416 978 Size: 416 562 NspI r_CATG'y Cuts at: 0 98 978 Size: 98 880 PasI CC'CwG_GG Cuts at: 0 178 594 978 Size: 178 416 384 Fragments arranged by size: 416 384 178 PciI A'CATG_T Cuts at: 0 94 978 Size: 94 884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 212 244 978 Size: 212 32 734 Fragments arranged by size: 734 212 32 PpuMI rG'GwC_Cy Cuts at: 0 357 883 978 Size: 357 526 95 Fragments arranged by size: 526 357 95 PshAI GACnn'nnGTC Cuts at: 0 95 978 Size: 95 883 PspOMI G'GGCC_C Cuts at: 0 173 262 978 Size: 173 89 716 Fragments arranged by size: 716 173 89 PvuII CAG'CTG Cuts at: 0 546 978 Size: 546 432 SacI G_AGCT'C Cuts at: 0 611 978 Size: 611 367 SanDI GG'GwC_CC Cuts at: 0 883 978 Size: 883 95 SexAI A'CCwGG_T Cuts at: 0 620 978 Size: 620 358 SmaI CCC'GGG Cuts at: 0 28 161 171 978 Size: 28 133 10 807 Fragments arranged by size: 807 133 28 10 StyI C'CwwG_G Cuts at: 0 20 978 Size: 20 958 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 290 978 Size: 290 688 TatI w'GTAC_w Cuts at: 0 656 692 978 Size: 656 36 286 Fragments arranged by size: 656 286 36 XmaI C'CCGG_G Cuts at: 0 26 159 169 978 Size: 26 133 10 809 Fragments arranged by size: 809 133 26 10 Enzymes that do cut: AarI AccI AflIII AlfI AloI ApaI AvaI BanII BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstBI BtgI BtsI EcoICRI Eco57MI EcoO109I FalI Hin4I MspA1I NaeI NcoI NgoMIV NspI PasI PciI PpiI PpuMI PshAI PspOMI PvuII SacI SanDI SexAI SmaI StyI TaqII TatI XmaI Enzymes that do not cut: AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NdeI NheI NotI NruI NsiI PacI PflMI PfoI PmeI PmlI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI ZraI