################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI Alw44I AlwNI AocI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII BalI BamHI BbrPI BclI BfrI BglII BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspMI BsrDI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HindIII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AhdI 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 426 AGCGCT AGC^GCT ApaI 84 GGGCCC GGGCC^C AspEI 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 511 GTGCTC G[AT]GC[AT]^C AvaI 148 CTCGGG C^[CT]CG[GA]G AvrII 699 CCTAGG C^CTAGG BbsI 754 GTCTTC GAAGAC(2/6) BbvCI 585 CCTCAGC CCTCAGC(-5/-2) BciVI 884 GTATCC GTATCC(6/5) BlnI 699 CCTAGG C^CTAGG BlpI 141 GCTCAGC GC^T[ACGT]AGC BpmI 117 CTCCAG CTGGAG(16/14) Bpu1102I 141 GCTCAGC GC^T[ACGT]AGC BpuAI 754 GTCTTC GAAGAC(2/6) BsaI 812 GGTCTC GGTCTC(1/5) BseRI 606 CTCCTC GAGGAG(10/8) BsiHKAI 511 GTGCTC G[AT]GC[AT]^C BsmAI 813 GTCTC GTCTC(1/5) BsoBI 148 CTCGGG C^[CT]CG[GA]G BspHI 596 TCATGA T^CATGA BspLU11I 1082 ACATGT A^CATGT BsrBI 1295 CCGCTC CCGCTC(-3/-3) BssHII 413 GCGCGC G^CGCGC CelII 141 GCTCAGC GC^T[ACGT]AGC EaeI 497 TGGCCG [CT]^GGCC[GA] Eam1105I 990 GACTGTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 426 AGCGCT AGC^GCT EcoNI 922 CCTTTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 699 CCTAGG C^C[AT][AT]GG HgaI 752 GCGTC GACGC(5/10) HincII 568 GTTGAC GT[CT]^[GA]AC HindII 568 GTTGAC GT[CT]^[GA]AC HphI 344 TCACC GGTGA(8/7) MseI 1138 TTAA T^TAA NaeI 124 GCCGGC GCC^GGC NgoMIV 124 GCCGGC G^CCGGC PpuMI 864 AGGACCT [GA]G^G[AT]CC[CT] RcaI 596 TCATGA T^CATGA SapI 27 GCTCTTC GCTCTTC(1/4) StyI 699 CCTAGG C^C[AT][AT]GG Tru9I 1138 TTAA T^TAA TspRI 1262 CAGTG CA[GC]TG[ACGT][ACGT]^ XbaI 1185 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AfaI 1031 GTAC GT^AC 1080 GTAC AflIII 317 ACGTGT A^C[GA][CT]GT 1082 ACATGT Asp700I 315 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 378 GAACCACTTC BcgI 92 GCACGGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 393 GCACGTCCGTCG Bsp1407I 1030 TGTACA T^GTACA 1079 TGTACA BsrFI 124 GCCGGC [GA]^CCGG[CT] 850 ACCGGC BsrGI 1030 TGTACA T^GTACA 1079 TGTACA BstXI 74 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 611 CCACGCTTCTGG BstYI 650 GGATCT [GA]^GATC[CT] 1179 AGATCC Cfr10I 124 GCCGGC [GA]^CCGG[CT] 850 ACCGGC DraII 84 GGGCCCC [GA]G^G[ACGT]CC[CT] 864 AGGACCT EcoO109I 84 GGGCCCC [GA]G^G[ACGT]CC[CT] 864 AGGACCT HinfI 717 GACTC G^A[ACGT]TC 825 GACTC MflI 650 GGATCT [GA]^GATC[CT] 1179 AGATCC NspI 1076 GCATGT [GA]CATG^[CT] 1082 ACATGT PleI 717 GACTC GAGTC(4/5) 825 GACTC PvuII 657 CAGCTG CAG^CTG 893 CAGCTG RsaI 1031 GTAC GT^AC 1080 GTAC SspBI 1030 TGTACA T^GTACA 1079 TGTACA Tsp509I 912 AATT ^AATT 1064 AATT XcmI 347 CCATGCTGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1037 CCATTCTAAAACTGG XhoII 650 GGATCT [GA]^GATC[CT] 1179 AGATCC XmnI 315 GAACGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 378 GAACCACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AvaII BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BsaHI BsaJI BsgI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PstI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI TseI Tsp45I