(Circular) (Six-base) MAPSORT of: pFN21AE6315.seq Check: 4762 from: 1 to: 6016 pFN21AE6315 6016 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2161 2928 4079 5463 5731 1632 Size: 529 767 1151 1384 268 1917 Fragments arranged by size: 1917 1384 1151 767 529 268 AfeI AGC'GCT Cuts at: 2433 2433 Size: 6016 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 2322 3087 4915 5892 1203 Size: 1119 765 1828 977 1327 Fragments arranged by size: 1828 1327 1119 977 765 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 3000 4745 3000 Size: 1745 4271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2369 2452 1184 Size: 1185 83 4748 Fragments arranged by size: 4748 1185 83 AlwNI CAG_nnn'CTG Cuts at: 3759 5331 3759 Size: 1572 4444 ApaI G_GGCC'C Cuts at: 2093 2093 Size: 6016 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 3325 3551 3703 5812 1493 Size: 1832 226 152 2109 1697 Fragments arranged by size: 2109 1832 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2153 3341 1870 Size: 73 210 1188 4545 Fragments arranged by size: 4545 1188 210 73 AvrII C'CTAG_G Cuts at: 2704 2704 Size: 6016 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2272 2333 3337 4792 Size: 325 217 627 281 204 61 1004 1455 Cuts at: 4792 618 Size: 1842 Fragments arranged by size: 1842 1455 1004 627 325 281 217 204 61 BanII G_rGCy'C Cuts at: 1391 2093 2148 2208 3335 1391 Size: 702 55 60 1127 4072 Fragments arranged by size: 4072 1127 702 60 55 BbeI G_GCGC'C Cuts at: 2072 2276 2337 2072 Size: 204 61 5751 Fragments arranged by size: 5751 204 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2752 928 Size: 971 853 4192 Fragments arranged by size: 4192 971 853 BbvCI CC'TCA_GC Cuts at: 2591 2591 Size: 6016 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2086 2120 2387 2421 4207 4241 1903 Size: 34 149 34 267 34 1786 34 3678 Fragments arranged by size: 3678 1786 267 149 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2900 3908 5118 2900 Size: 1008 1210 3798 Fragments arranged by size: 3798 1210 1008 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2226 2445 2510 Size: 107 122 71 631 605 554 219 65 Cuts at: 2510 4627 136 Size: 2117 1525 Fragments arranged by size: 2117 1525 631 605 554 219 122 107 71 65 BglII A'GATC_T Cuts at: 6011 6011 Size: 6016 BlpI GC'TnA_GC Cuts at: 2147 3433 2147 Size: 1286 4730 Bme1580I G_kGCm'C Cuts at: 1790 2093 4077 5233 1790 Size: 303 1984 1156 2573 Fragments arranged by size: 2573 1984 1156 303 BmgBI CAC'GTC Cuts at: 1318 2380 2401 1318 Size: 1062 21 4933 Fragments arranged by size: 4933 1062 21 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3747 4705 442 Size: 416 53 83 399 24 2330 958 1753 Fragments arranged by size: 2330 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2107 4676 1239 Size: 868 2569 2579 Fragments arranged by size: 2579 2569 868 Bpu10I CC'TnA_GC Cuts at: 2591 2591 Size: 6016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4141 5006 5304 4141 Size: 865 298 4853 Fragments arranged by size: 4853 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2823 4679 882 Size: 617 1324 1856 2219 Fragments arranged by size: 2219 1856 1324 617 BsaAI yAC'GTr Cuts at: 493 1204 3807 493 Size: 711 2603 2702 Fragments arranged by size: 2702 2603 711 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 2273 2334 2757 Size: 53 83 186 936 536 204 61 423 Cuts at: 2757 3823 4205 4840 275 Size: 1066 382 635 1451 Fragments arranged by size: 1451 1066 936 635 536 423 382 204 186 83 61 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5791 Fragments arranged by size: 5791 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2602 1186 Size: 1416 4600 BseYI C'CCAG_C Cuts at: 2356 2664 5219 5715 2356 Size: 308 2555 496 2657 Fragments arranged by size: 2657 2555 496 308 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2353 2711 3047 1163 Size: 573 617 358 336 4132 Fragments arranged by size: 4132 617 573 358 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 4077 4162 5233 2520 Size: 1557 85 1071 3303 Fragments arranged by size: 3303 1557 1071 85 BsmI GAATG_Cn' Cuts at: 3527 3620 3527 Size: 93 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2148 2208 2520 3335 4077 4162 Size: 399 303 55 60 312 815 742 85 Cuts at: 4162 5233 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 815 742 399 312 303 85 60 55 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 2601 3905 5635 2601 Size: 1304 1730 2982 Fragments arranged by size: 2982 1730 1304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3371 844 Size: 2527 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3302 3903 1394 Size: 429 1479 601 3507 Fragments arranged by size: 3507 1479 601 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4511 4685 65 Size: 1183 3263 174 1396 Fragments arranged by size: 3263 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 2855 4660 4847 1700 Size: 110 319 726 1805 187 2869 Fragments arranged by size: 2869 1805 726 319 187 110 BsrGI T'GTAC_A Cuts at: 96 3035 3084 96 Size: 2939 49 3028 Fragments arranged by size: 3028 2939 49 BssHII G'CGCG_C Cuts at: 1925 2418 1925 Size: 493 5523 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6016 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2623 2086 Size: 537 5479 BstYI r'GATC_y Cuts at: 1972 2655 3184 3346 3713 4098 4115 5567 5653 Size: 683 529 162 367 385 17 1452 86 Cuts at: 5653 5665 6011 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 683 529 385 367 346 162 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2242 2311 2412 2508 513 Size: 552 939 238 69 101 96 4021 Fragments arranged by size: 4021 939 552 238 101 96 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5277 Fragments arranged by size: 5277 683 56 BtsI GCAGTG_nn' Cuts at: 3532 4326 4346 3532 Size: 794 20 5202 Fragments arranged by size: 5202 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2502 3463 4352 Size: 54 1425 50 275 18 672 961 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 961 889 672 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2972 3231 3946 1353 Size: 686 933 259 715 3423 Fragments arranged by size: 3423 933 715 686 259 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4603 4977 5123 387 Size: 1064 138 3014 374 146 1280 Fragments arranged by size: 3014 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2107 2161 2928 4079 4676 5463 5731 Size: 393 475 54 767 1151 597 787 268 Cuts at: 5731 1239 Size: 1524 Fragments arranged by size: 1524 1151 787 767 597 475 393 268 54 EcoNI CCTnn'n_nnAGG Cuts at: 2931 2931 Size: 6016 EcoO109I rG'GnC_Cy Cuts at: 2090 2870 2090 Size: 780 5236 EcoRI G'AATT_C Cuts at: 3325 3703 3325 Size: 378 5638 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 2072 2130 2276 2337 2435 5163 1678 Size: 394 58 146 61 98 2728 2531 Fragments arranged by size: 2728 2531 394 146 98 61 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2707 2739 2986 3018 4657 Size: 32 135 32 1135 32 247 32 1639 Cuts at: 4657 4689 4731 4763 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1639 1135 247 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2575 3360 3606 669 Size: 988 281 637 785 246 3079 Fragments arranged by size: 3079 988 785 637 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6016 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KasI G'GCGC_C Cuts at: 2068 2272 2333 2068 Size: 204 61 5751 Fragments arranged by size: 5751 204 61 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2219 5130 5314 1305 Size: 914 2911 184 2007 Fragments arranged by size: 2911 2007 914 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4194 Fragments arranged by size: 4194 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 2600 3086 3974 4333 4492 518 Size: 648 1185 249 486 888 359 159 2042 Fragments arranged by size: 2042 1185 888 648 486 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2217 2305 2458 2664 2900 3432 4109 5257 Size: 611 88 153 206 236 532 677 1148 Cuts at: 5257 5502 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 611 532 245 236 206 153 88 NaeI GCC'GGC Cuts at: 2131 2131 Size: 6016 NarI GG'CG_CC Cuts at: 2069 2273 2334 2069 Size: 204 61 5751 Fragments arranged by size: 5751 204 61 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4525 Fragments arranged by size: 4525 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 3085 3091 3374 4919 1132 Size: 97 1856 6 283 1545 2229 Fragments arranged by size: 2229 1856 1545 283 97 6 PasI CC'CwG_GG Cuts at: 1348 2141 2268 1348 Size: 793 127 5096 Fragments arranged by size: 5096 793 127 PciI A'CATG_T Cuts at: 3087 4915 3087 Size: 1828 4188 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6016 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2870 2870 Size: 6016 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3222 3586 3222 Size: 364 5652 PspOMI G'GGCC_C Cuts at: 2089 2089 Size: 6016 PstI C_TGCA'G Cuts at: 830 2732 2899 3124 3368 830 Size: 1902 167 225 244 3478 Fragments arranged by size: 3478 1902 244 225 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2664 2900 2664 Size: 236 5780 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2039 2039 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 4264 5976 4264 Size: 1712 4304 SfcI C'TryA_G Cuts at: 826 1045 2659 2728 2895 3120 3364 4499 5180 Size: 219 1614 69 167 225 244 1135 681 Cuts at: 5180 5371 826 Size: 191 1471 Fragments arranged by size: 1614 1471 1135 681 244 225 219 191 167 69 SfoI GGC'GCC Cuts at: 2070 2274 2335 2070 Size: 204 61 5751 Fragments arranged by size: 5751 204 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 3343 1872 Size: 1471 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4120 5021 5283 820 Size: 197 926 2177 901 262 1553 Fragments arranged by size: 2177 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6016 StyI C'CwwG_G Cuts at: 513 1065 2004 2704 3455 513 Size: 552 939 700 751 3074 Fragments arranged by size: 3074 939 751 700 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2995 4043 4060 4213 4398 4843 325 Size: 1255 1415 1048 17 153 185 445 1498 Fragments arranged by size: 1498 1415 1255 1048 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3035 3084 4262 Size: 274 80 33 51 1443 1058 49 1178 Cuts at: 4262 5974 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1178 1058 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2234 2266 5976 6008 2234 Size: 32 3710 32 2242 Fragments arranged by size: 3710 2242 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3190 3352 3190 Size: 162 5854 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 3049 2359 Size: 690 5326 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 3341 1870 Size: 1471 4545 XmnI GAAnn'nnTTC Cuts at: 2324 2387 4145 2324 Size: 63 1758 4195 Fragments arranged by size: 4195 1758 63 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI